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RESEARCH ARTICLE (Open Access)

Physical treatment and protease or probiotic supplementation and feather meal digestibility by broilers

Y. M. Sun https://orcid.org/0000-0002-9887-2303 A * , X. Li https://orcid.org/0000-0003-3109-5789 A , D. Zhang A and W. L. Bryden https://orcid.org/0000-0002-7187-4464 A B
+ Author Affiliations
- Author Affiliations

A School of Agriculture and Food Sustainability, The University of Queensland, Gatton, Qld 4343, Australia.

B Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Gatton, Qld 4343, Australia.

* Correspondence to: yiman.sun@uq.net.au

Handling Editor: Teck Loh

Animal Production Science 64, AN24091 https://doi.org/10.1071/AN24091
Submitted: 20 March 2024  Accepted: 20 April 2024  Published: 16 May 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Context

Improving the utilisation of alternative protein ingredients in livestock production will reduce feeding costs and improve industry sustainability. Feather meal (FM) is an abundant, alternative protein source with a high protein content but poor amino acid (AA) digestibility.

Aim

This study evaluated strategies for improving AA digestibility of FM.

Methods

Experiment 1 examined the effects of physical treatment with ultrasound, microwave and autoclaving on FM AA profile and digestibility. Experiment 2 evaluated the dietary addition of a protease (Ronozyme ProAct, 200 and 600 mg/kg; RPA) and a probiotic (BioPlus 400, 1500 mg/kg) on FM AA digestibility. Apparent ileal digestibility was determined by feeding each treatment to four replicate groups of six birds in Experiment 1 and five replicate groups of seven birds in Experiment 2, and then collecting the contents of the lower half of the ileum.

Key results

None of the physical treatments improved (P > 0.05) the AA profile or ileal AA digestibility of FM. Dietary supplementation with RPA at 200 mg/kg or BioPlus 400 at 1500 mg/kg did not significantly (P > 0.05) influence the apparent ileal AA digestibility of FM. However, the higher concentration of RPA (600 mg/kg) significantly (P < 0.05) increased the apparent ileal AA digestibility of FM.

Conclusion

The increased digestibility of FM by the protease and numerical increase (P < 0.1) by the probiotic (1500 mg/kg) presumably reflects keratinase activity of both feed supplements.

Implications

The results of this study indicated that there is scope for further improvement in the nutritive value of FM for broilers.

Keywords: amino acid digestibility, autoclave, broilers, feather meal, microwave, probiotic, protease, ultrasound.

References

Angel CR, Saylor W, Vieira SL, Ward N (2011) Effects of a monocomponent protease on performance and protein utilization in 7 to 22 day-old broiler chickens. Poultry Science 90, 2281-2286.
| Crossref | Google Scholar | PubMed |

Awad W, Ghareeb K, Böhm J (2008) Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides. International Journal of Molecular Sciences 9, 2205-2216.
| Crossref | Google Scholar | PubMed |

Bai S, Wu A, Ding X, Lei Y, Bai J, Zhang K, Chio J (2013) Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry Science 92, 663-670.
| Crossref | Google Scholar | PubMed |

Bajagai YS, Yeoh YK, Li X, Zhang D, Dennis PG, Ouwerkerk D, Dart PJ, Klieve AV, Bryden WL (2023) Enhanced meat chicken productivity in response to the probiotic Bacillus amyloliquefaciens H57 is associated with the enrichment of microbial amino acid and vitamin biosynthesis pathways. Journal of Applied Microbiology 134, lxad085.
| Crossref | Google Scholar |

Bandegan A, Kiarie E, Payne RL, Crow GH, Guenter W, Nyachoti CM (2010) Standardized ileal amino acid digestibility in dry-extruded expelled soybean meal, extruded canola seed-pea, feather meal, and poultry by-product meal for broiler chickens. Poultry Science 89, 2626-2633.
| Crossref | Google Scholar | PubMed |

Bedford MR, Morgan AJ (1996) The use of enzymes in poultry diets. World’s Poultry Science Journal 52, 61-68.
| Crossref | Google Scholar |

Bertechini AG, de Carvalho JCC, Carvalho AC, Dalolio FS, Sorbara JOB (2020) Amino acid digestibility coefficient values of animal protein meals with dietary protease for broiler chickens. Translational Animal Science 4, txaa187.
| Crossref | Google Scholar |

Brandelli A (2008) Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond. Food and Bioprocess Technology 1, 105-116.
| Crossref | Google Scholar |

Brandelli A, Daroit DJ, Riffel A (2010) Biochemical features of microbial keratinases and their production and applications. Applied Microbiology and Biotechnology 85, 1735-1750.
| Crossref | Google Scholar | PubMed |

Cai G, Moffitt K, Navone L, Zhang Z, Robins K, Speight R (2022) Valorisation of keratin waste: controlled pretreatment enhances enzymatic production of antioxidant peptides. Journal of Environmental Management 301, 113945.
| Crossref | Google Scholar | PubMed |

Chen L, Wang N, Sun D, Li L (2014) Microwave-assisted acid hydrolysis of proteins combined with peptide fractionation and mass spectrometry analysis for characterizing protein terminal sequences. Journal of Proteomics 100, 68-78.
| Crossref | Google Scholar | PubMed |

Chen J, Ding S, Ji Y, Ding J, Yang X, Zou M, Li Z (2015) Microwave-enhanced hydrolysis of poultry feather to produce amino acid. Chemical Engineering and Processing: Process Intensification 87, 104-109.
| Crossref | Google Scholar |

Cherry JP, Young CT, Shewfelt AL (1975) Characterization of protein isolates from keratinous material of poultry feathers. Journal of Food Science 40, 331-335.
| Crossref | Google Scholar |

Cowieson AJ, Roos FF (2013) Bioefficacy of a mono-component protease in the diets of pigs and poultry: a meta-analysis of effect on ileal amino acid digestibility. Journal of Applied Animal Nutrition 2, e13.
| Crossref | Google Scholar |

Cowieson AJ, Roos FF (2016) Toward optimal value creation through the application of exogenous mono-component protease in the diets of non-ruminants. Animal Feed Science and Technology 221, 331-340.
| Crossref | Google Scholar |

Dai L, Wang Y, Liu Y, Ruan R (2020) Microwave-assisted pyrolysis of formic acid pretreated bamboo sawdust for bio-oil production. Environmental Research 182, 108988.
| Crossref | Google Scholar | PubMed |

Daroit DJ, Corrêa APF, Segalin J, Brandelli A (2010) Characterization of a keratinolytic protease produced by the feather-degrading Amazonian bacterium Bacillus sp. P45. Biocatalysis and Biotransformation 28, 370-379.
| Crossref | Google Scholar |

El Boushy AR, van der Poel AFB, Walraven OED (1990) Feather meal – a biological waste: its processing and utilization as a feedstuff for poultry. Biological Wastes 32, 39-74.
| Crossref | Google Scholar |

Fru-Nji F, Kluenter A-M, Fischer M, Pontoppidan K (2011) A feed serine protease improves broiler performance and increases protein and energy digestibility. The Journal of Poultry Science 48, 239-246.
| Crossref | Google Scholar |

Glitsø V, Pontoppidan K, Knap I, Ward N (2012) Development of a feed protease. Industrial Biotechnology 8, 172-175.
| Crossref | Google Scholar |

Grazziotin A, Pimentel FA, de Jong EV, Brandelli A (2006) Nutritional improvement of feather protein by treatment with microbial keratinase. Animal Feed Science and Technology 126, 135-144.
| Crossref | Google Scholar |

Han Y, Parsons C (1991) Protein and amino acid quality of feather meals. Poultry Science 70, 812-822.
| Crossref | Google Scholar |

Kaewtapee C, Burbach K, Tomforde G, Hartinger T, Camarinha-Silva A, Heinritz S, Seifert J, Wiltafsky M, Mosenthin R, Rosenfelder-Kuon P (2017) Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs. Journal of Animal Science and Biotechnology 8, 37.
| Crossref | Google Scholar |

Kamel NF, Naela, Ragaa M, El-Banna R, Mohamed F (2015) Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens. Agriculture and Agricultural Science Procedia 6, 216-225.
| Crossref | Google Scholar |

Kreplak L, Doucet J, Dumas P, Briki F (2004) New aspects of the α-helix to β-sheet transition in stretched hard α-keratin fibers. Biophysical Journal 87, 640-647.
| Crossref | Google Scholar | PubMed |

Latshaw JD, Musharaf N, Retrum R (1994) Processing of feather meal to maximize its nutritional value for poultry. Animal Feed Science and Technology 47, 179-188.
| Crossref | Google Scholar |

Lee YS, Phang L-Y, Ahmad SA, Ooi PT (2016) Microwave–alkali treatment of chicken feathers for protein hydrolysate production. Waste and Biomass Valorization 7, 1147-1157.
| Crossref | Google Scholar |

Li Q (2019) Progress in microbial degradation of feather waste. Frontiers in Microbiology 10, 2717.
| Crossref | Google Scholar | PubMed |

Li X, Higgins TJV, Bryden WL (2006) Biological response of broiler chickens fed peas (Pisum sativum) expressing the bean (Phaseolus vulgaris L.) α-amylase inhibitor transgene. Journal of the Science of Food and Agriculture 86, 1900-1907.
| Crossref | Google Scholar |

Li J-F, Wei F, Dong X-Y, Guo L-L, Yuan G-Y, Huang F-H, Jiang M-L, Zhao Y-D, Li G-M, Chen H (2010) Microwave-assisted approach for the rapid enzymatic digestion of rapeseed meal. Food Science and Biotechnology 19, 463-469.
| Crossref | Google Scholar |

Lindberg D, Kristoffersen KA, Wubshet SG, Hunnes LMG, Dalsnes M, Dankel KR, Høst V, Afseth NK (2021) Exploring effects of protease choice and protease combinations in enzymatic protein hydrolysis of poultry by-products. Molecules 26, 5280.
| Crossref | Google Scholar | PubMed |

Liu X, Yan H, Le L, Xu C, Yin C, Zhang K, Wang P, Hu J (2012) Growth performance and meat quality of broiler chickens supplemented with Bacillus licheniformis in drinking water. Asian-Australasian Journal of Animal Sciences 25, 682-689.
| Crossref | Google Scholar | PubMed |

Luo J, Zheng A, Meng K, Chang W, Bai Y, Li K, Cai H, Liu G, Yao B (2014) Proteome changes in the intestinal mucosa of broiler (Gallus gallus) activated by probiotic Enterococcus faecium. Journal of Proteomics 91, 226-241.
| Crossref | Google Scholar |

Luong VB, Payne CG (1977) Hydrolysed feather protein as a source of amino acids for laying hens. British Poultry Science 8, 523-526.
| Crossref | Google Scholar |

MacAlpine R, Payne CG (1977) Hydrolysed feather protein as a source of amino acids for broilers. British Poultry Science 18, 265-273.
| Crossref | Google Scholar |

Mazotto AM, Coelho RRR, Cedrola SML, de Lima MF, Couri S, Paraguai de Souza E, Vermelho AB (2011) Keratinase production by three Bacillus spp. using feather meal and whole feather as substrate in a submerged fermentation. Enzyme Research 2011, 523780.
| Crossref | Google Scholar |

Moritz JS, Latshaw JD (2001) Indicators of nutritional value of hydrolyzed feather meal. Poultry Science 80, 79-86.
| Crossref | Google Scholar | PubMed |

Navone L, Speight R (2018) Understanding the dynamics of keratin weakening and hydrolysis by proteases. PLoS ONE 13, e0202608.
| Crossref | Google Scholar | PubMed |

NHMRC (2013) Australian code for the care and use of animals for scientific purposes. 8th Edn, National Health and Medical Research Council, Canberra, ACT. Australia.

Onifade AA, Al-Sane NA, Al-Musallam AA, Al-Zarban S (1998) A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresource Technology 66, 1-11.
| Crossref | Google Scholar |

Papadopoulos MC, El Boushy AR, Ketelaars EH (1985) Effect of different processing conditions on amino acid digestibility of feather meal determined by chicken assay. Poultry Science 64, 1729-1741.
| Crossref | Google Scholar |

Papadopoulos MC, El Boushy AR, Roodbeen AE, Ketelaars EH (1986) Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal. Animal Feed Science and Technology 14, 279-290.
| Crossref | Google Scholar |

Parry DA, North A (1998) Hard alpha-keratin intermediate filament chains: substructure of the N-and C-terminal domains and the predicted structure and function of the C-terminal domains of type I and type II chains. Journal of Structural Biology 122, 67-75.
| Crossref | Google Scholar | PubMed |

Patist A, Bates D (2008) Ultrasonic innovations in the food industry: from the laboratory to commercial production. Innovative Food Science & Emerging Technologies 9, 147-154.
| Crossref | Google Scholar |

Polin D (1992) Feathers, feather meal and other poultry by-products. In ‘Advances in meat research, Vol. 8, inedible meat by-products’. (Eds AM Pearson, TR Dutson) pp. 177–198. (Elsevier Inc.: New York, NY, USA)

Qin X, Yang C, Guo Y, Liu J, Bitter JH, Scott EL, Zhang C (2023) Effect of ultrasound on keratin valorization from chicken feather waste: process optimization and keratin characterization. Ultrasonics Sonochemistry 93, 106297.
| Crossref | Google Scholar |

Ravindran V (2013) Feed enzymes: The science, practice, and metabolic realities. Journal of Applied Poultry Research 22, 628-636.
| Crossref | Google Scholar |

Ravindran V, Hew LI, Ravindran G, Bryden WL (1999) A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry. British Poultry Science 40, 266-274.
| Crossref | Google Scholar | PubMed |

Ravindran V, Hew LI, Ravindran G, Bryden WL (2005) Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens. Animal Science 81, 85-97.
| Crossref | Google Scholar |

Rodríguez-Clavel IS, Paredes-Carrera SP, Flores-Valle SO, Paz-García EJ, Sánchez-Ochoa JC, Pérez-Gutiérrez RM (2019) Effect of microwave or ultrasound irradiation in the extraction from feather keratin. Journal of Chemistry 2019, 1326063.
| Crossref | Google Scholar |

Romero L, Plumstead P (2013) Bio-efficacy of feed proteases in poultry and their interaction with other feed enzymes. Proceedings of the Australian Poultry Science Symposium 24, 23-30.
| Google Scholar |

Saleh AA, Amber K, Mohammed AA (2020a) Dietary supplementation with avilamycin and Lactobacillus acidophilus effects growth performance and the expression of growth-related genes in broilers. Animal Production Science 60, 1704-1710.
| Crossref | Google Scholar |

Saleh AA, Dawood MM, Badawi NA, Ebeid TA, Amber KA, Azzam MM (2020b) Effect of supplemental serine-protease from Bacillus licheniformis on growth performance and physiological change of broiler chickens. Journal of Applied Animal Research 48, 86-92.
| Crossref | Google Scholar |

Shini S, Bryden WL (2022) Probiotics and gut health: linking gut homeostasis and poultry productivity. Animal Production Science 62, 1090-1112.
| Crossref | Google Scholar |

Shini S, Acland RC, Bryden WL (2021) Avian intestinal ultrastructure changes provide insight into the pathogenesis of enteric infections and probiotic mode of action. Scientific Reports 11, 167.
| Crossref | Google Scholar | PubMed |

Sun X, Ohanenye IC, Ahmed T, Udenigwe CC (2020) Microwave treatment increased protein digestibility of pigeon pea (Cajanus cajan) flour: elucidation of underlying mechanisms. Food Chemistry 329, 127196.
| Crossref | Google Scholar | PubMed |

Šabatková J, Kumprecht I, Zobač P, Suchý P, Čermák B (2008) The probiotic BioPlus 2B as an alternative to antibiotics in diets for broiler chickens. Acta Veterinaria Brno 77, 569-574.
| Crossref | Google Scholar |

Sweeney RA (1989) Generic combustion method for determination of crude protein in feeds: Collaborative study. Journal of Association of Official Analytical Chemists 72, 770-774.
| Crossref | Google Scholar |

Yang J, Kun Q, Dong W, Zhang W, Wu Y, Xu Y (2017) Effects of different proportions of two Bacillus sp. on the growth performance, small intestinal morphology, caecal microbiota and plasma biochemical profile of Chinese Huainan Partridge Shank chickens. Journal of Integrative Agriculture 16, 1383-1392.
| Crossref | Google Scholar |

Zaghari M, Sarani P, Hajati H (2020) Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens. Journal of Applied Animal Research 48, 166-175.
| Crossref | Google Scholar |

Zheng M, Bai Y, Sun Y, An J, Chen Q, Zhang T (2023) Effects of different proteases on protein digestion in vitro and in vivo and growth performance of broilers fed corn–soybean meal diets. Animals 13, 1746.
| Crossref | Google Scholar | PubMed |

Zoccola M, Aluigi A, Patrucco A, Vineis C, Forlini F, Locatelli P, Sacchi MC, Tonin C (2012) Microwave-assisted chemical-free hydrolysis of wool keratin. Textile Research Journal 82, 2006-2018.
| Crossref | Google Scholar |