Effects of guanidinoacetic acid on the growth performance, meat quality, postmortem energy metabolism and muscle fibre types of finishing pigs
Jingzheng Li
A , Jiaolong Li A B * , Lin Zhang A , Tong Xing A , Yun Jiang C and Feng Gao A *
A
B
C
Abstract
Meat quality is increasingly being paid more attention by customers and enterprises. However, the modern pursuit of pork production has led to a decline in pork quality. Muscle fibre type is one of the important factors affecting meat quality that can be used as a key control point.
This study set out to assess the effects of dietary guanidinoacetic acid (GAA) on the growth performance, meat quality, postmortem energy metabolism, and muscle fibre types of finishing pigs.
In total, 180 healthy Duroc × Landrace × Meishan cross castrated male pigs with the similar weight (average 90 kg) were randomly divided into three treatments, with five replicates (pens) per treatment and 12 pigs per pen, including a GAA-free basal diet and basal diet with 0.05% or 0.10% GAA for 15 days.
In longissimus thoracis muscle, the results indicated that GAA supplementation decreased the drip loss and the cooking loss in 0.10% GAA group. Meanwhile, in semitendinosus muscle, 0.10% GAA addition increased pH45 min, and decreased the cooking loss. Additionally, GAA addition increased the content of ATP and AMP in semitendinosus muscle. The mRNA expressions of MyHC-I and MyHC-IIa were increased, whereas MyHC-IIx and MyHC-IIb were decreased. Moreover, in longissimus thoracis muscle, GAA addition promoted the mRNA expressions of CaM and NFATc1; in semitendinosus muscle, dietary GAA up-regulated the CnA and NFATc1 mRNA expressions.
GAA addition improved the meat quality, enhanced postmortem energy metabolism and promoted the conversion of fast-muscle fibre to slow-muscle fibre via activating the CaN/NFAT signalling.
The addition of GAA can improve the meat quality of post-slaughter finishing pigs and provide a theoretical basis for the application of GAA in livestock production.
Keywords: CaN/NFAT, finishing pigs, growth performance, guanidinoacetic acid, meat quality, muscle fibre types, muscle glycolysis, postmortem energy metabolism.
References
Andreo N, Bridi AM, Peres LM, Dos Santos ÉR, Barro AG, Gonçalves JRS, Bonfá HC, Pires AV (2019) Carcass characteristics and meat quality of Nellore bulls submitted to different nutritional strategies during cow–calf and stockerphase. Animal 13, 1544-1551.
| Crossref | Google Scholar |
Brookes PS, Yoon Y, Robotham JL, Anders MW, Sheu S-S (2004) Calcium, ATP, and ROS: a mitochondrial love–hate triangle. American Journal of Physiology-Cell Physiology 287, C817-C833.
| Crossref | Google Scholar | PubMed |
Çenesiz AA, Yavaş İ, Çiftci İ, Ceylan N, Taşkesen HO (2020) Guanidinoacetic acid supplementation is favourable to broiler diets even containing poultry by-product meal. British Poultry Science 61, 311-319.
| Crossref | Google Scholar | PubMed |
Choe JH, Choi YM, Lee SH, Shin HG, Ryu YC, Hong KC, Kim BC (2008) The relation between glycogen, lactate content and muscle fiber type composition, and their influence on postmortem glycolytic rate and pork quality. Meat Science 80, 355-362.
| Crossref | Google Scholar |
Córdova-Noboa HA, Oviedo-Rondón EO, Sarsour AH, Barnes J, Sapcota D, López D, Gross L, Rademacher-Heilshorn M, Braun U (2018) Effect of guanidinoacetic acid supplementation on live performance, meat quality, pectoral myopathies and blood parameters of male broilers fed corn-based diets with or without poultry by-products. Poultry Science 97, 2494-2505.
| Crossref | Google Scholar | PubMed |
DeGroot AA, Braun U, Dilger RN (2018) Efficacy of guanidinoacetic acid on growth and muscle energy metabolism in broiler chicks receiving arginine-deficient diets. Poultry Science 97, 890-900.
| Crossref | Google Scholar |
Degroot AA, Braun U, Dilger RN (2019) Guanidinoacetic acid is efficacious in improving growth performance and muscle energy homeostasis in broiler chicks fed arginine-deficient or arginine-adequate diets. Poultry Science 98, 2896-2905.
| Crossref | Google Scholar | PubMed |
Edison EE, Brosnan ME, Meyer C, Brosnan JT (2007) Creatine synthesis: production of guanidinoacetate by the rat and human kidney in vivo. American Journal of Physiology – Renal Physiology 293, F1799-F1804.
| Google Scholar |
Gallo M, MacLean I, Tyreman N, Martins KJB, Syrotuik D, Gordon T, Putman CT (2008) Adaptive responses to creatine loading and exercise in fast-twitch rat skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 294, R1319-R1328.
| Crossref | Google Scholar |
Gattenlöhner S, Schneider C, Thamer C, Klein R, Roggendorf W, Gohlke F, Niethammer C, Czub S, Vincent A, Müller-Hermelink H-K, Marx A (2002) Expression of foetal type acetylcholine receptor is restricted to type 1 muscle fibres in human neuromuscular disorders. Brain 125, 1309-1319.
| Crossref | Google Scholar | PubMed |
Häussinger D, Gerok W (1994) Role of the cellular hydration state for cellular function: physiological and pathophysiological aspects. Advances in Experimental Medicine & Biology 368, 33-44.
| Google Scholar | PubMed |
He DT, Gai XR, Yang LB, Li JT, Lai WQ, Sun XL, Zhang LY (2018) Effects of guanidinoacetic acid on growth performance, creatine and energy metabolism, and carcass characteristics in growing-finishing pigs. Journal of Animal Science 96, 3264-3273.
| Crossref | Google Scholar | PubMed |
Inesi G, Tadini-Buoninsegni F (2014) Ca2+/H+ exchange, lumenal Ca2+ release and Ca2+/ATP coupling ratios in the sarcoplasmic reticulum ATPase. Journal of Cell Communication and Signaling 8, 5-11.
| Crossref | Google Scholar | PubMed |
Jayaraman B, La KV, La H, Doan V, Carpena EM, Rademacher M, Channarayapatna G (2018) Supplementation of guanidinoacetic acid to pig diets: effects on performance, carcass characteristics, and meat quality. Journal of Animal Science 96, 2332-2341.
| Crossref | Google Scholar | PubMed |
Khan M, Couturier A, Kubens JF, Most E, Mooren F-C, Krüger K, Ringseis R, Eder K (2013) Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep. Acta Veterinaria Scandinavica 55, 85.
| Crossref | Google Scholar | PubMed |
Li J, Zhang L, Fu Y, Li Y, Jiang Y, Zhou G, Gao F (2018) Creatine monohydrate and guanidinoacetic acid supplementation affects the growth performance, meat quality, and creatine metabolism of finishing pigs. Journal of Agricultural and Food Chemistry 66, 9952-9959.
| Crossref | Google Scholar | PubMed |
Li J, Li J, Zhang L, Xing T, Jiang Y, Gao F (2022) Guanidinoacetic acid supplementation promotes skeletal muscle fiber type transformation from fast-to-slow-twitch via increasing the PPARGC1A based mitochondrial function and CaN/NFAT pathway in finishing pigs. Agriculture 12, 87.
| Crossref | Google Scholar |
Lin J, Zhang C, Lu K, Song K, Wang L (2021) Effect of guanidinoacetic acid and betaine supplementation in soybean meal-based diets on growth performance, muscle energy metabolism and methionine utilization in the bullfrog Lithobates catesbeianus. Aquaculture 533, 736167.
| Crossref | Google Scholar |
Liu S, Liu P, Borras A, Chatila T, Speck SH (1997) Cyclosporin A-sensitive induction of the Epstein-Barr virus lytic switch is mediated via a novel pathway involving a MEF2 family member. EMBO Journal 16, 143-153.
| Crossref | Google Scholar | PubMed |
Liu YJ, Chen JZ, Wang DH, Wu MJ, Zheng C, Wu ZZ, Wang C, Liu Q, Zhang J, Guo G, Huo WJ (2020) Effects of guanidinoacetic acid and coated folic acid supplementation on growth performance, nutrient digestion and hepatic gene expression in Angus bulls. British Journal of Nutrition 126, 510-517.
| Crossref | Google Scholar |
Long YC, Glund S, Garcia-Roves PM, Zierath JR (2007) Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression. Journal of Biological Chemistry 282, 1607-1614.
| Crossref | Google Scholar | PubMed |
Lu Y, Zou T, Wang Z, Yang J, Li L, Guo X, He Q, Chen L, You J (2020) Dietary guanidinoacetic acid improves the growth performance and skeletal muscle development of finishing pigs through changing myogenic gene expression and myofibre characteristics. Journal of Animal Physiology and Animal Nutrition 104, 1875-1883.
| Crossref | Google Scholar | PubMed |
Luo P, Wang L, Luo L, Wang L, Yang K, Shu G, Wang S, Zhu X, Gao P, Jiang Q (2019) Ca2+-Calcineurin-NFAT pathway mediates the effect of thymol on oxidative metabolism and fiber-type switch in skeletal muscle. Food & Function 10, 5166-5173.
| Crossref | Google Scholar | PubMed |
McCullagh KJ, Calabria E, Pallafacchina G, Ciciliot S, Serrano AL, Argentini C, Kalhovde JM, Lømo T, Schiaffino S (2004) NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching. Proceedings of the National Academy of Sciences of the United States of America 101, 10590-10595.
| Crossref | Google Scholar |
Michiels J, Maertens L, Buyse J, Lemme A, Rademacher M, Dierick NA, De Smet S (2012) Supplementation of guanidinoacetic acid to broiler diets: effects on performance, carcass characteristics, meat quality, and energy metabolism. Poultry Science 91, 402-412.
| Crossref | Google Scholar | PubMed |
Pandorf CE, Jiang WH, Qin AX, Bodell PW, Baldwin KM, Haddad F (2010) Calcineurin plays a modulatory role in loading-induced regulation of type I myosin heavy chain gene expression in slow skeletal muscle. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology 297, R1037-R1048.
| Crossref | Google Scholar |
Pette D, Staron RS (2001) Transitions of muscle fiber phenotypic profiles. Histochemistry and Cell Biology 115, 359-372.
| Crossref | Google Scholar |
Pette D, Staron RS (2015) Myosin isoforms, muscle fiber types, and transitions. Microscopy Research & Technique 50(6), 500-509.
| Crossref | Google Scholar |
Schulz RA, Yutzey KE (2004) Calcineurin signaling and NFAT activation in cardiovascular and skeletal muscle development. Developmental Biology 266, 1-16.
| Crossref | Google Scholar | PubMed |
Silva JA, Patarata L, Martins C (1999) Influence of ultimate pH on bovine meat tenderness during ageing. Meat Science 52, 453-459.
| Crossref | Google Scholar | PubMed |
Suman SP, Joseph P (2013) Myoglobin chemistry and meat color. Annual Review of Food Science and Technology 4, 79-99.
| Crossref | Google Scholar | PubMed |
Tang K, Wagner PD, Breen EC (2010) TNF-α-mediated reduction in PGC-1-α may impair skeletal muscle function after cigarette smoke exposure. Journal of Cellular Physiology 222, 320-327.
| Crossref | Google Scholar | PubMed |
Wang X, Li J, Cong J, Chen X, Zhu X, Zhang L, Gao F, Zhou G (2017) Preslaughter transport effect on broiler meat quality and post-mortem glycolysis metabolism of muscles with different fiber types. Journal of Agricultural and Food Chemistry 65, 10310-10316.
| Crossref | Google Scholar | PubMed |
Wyss M, Kaddurah-Daouk R (2000) Creatine and creatinine metabolism. Physiological Reviews 80, 1107-1213.
| Crossref | Google Scholar |
Yu QP, Feng DY, He XJ, Wu F, Xia MH, Dong T, Liu YH, Tan HZ, Zou SG, Zheng T, Ou XH, Zuo JJ (2017) Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes. Asian–Australasian Journal of Animal Sciences 30, 1620-1632.
| Crossref | Google Scholar |
Zhang L, Li JL, Wang XF, Zhu XD, Gao F, Zhou GH (2019) Attenuating effects of guanidinoacetic acid on preslaughter transport-induced muscle energy expenditure and rapid glycolysis of broilers. Poultry Science 98, 3223-3232.
| Crossref | Google Scholar | PubMed |
Zhu Z, Gu C, Hu S, Li B, Zeng X, Yin J (2020) Dietary guanidinoacetic acid supplementation improved carcass characteristics, meat quality and muscle fibre traits in growing-finishing gilts. Journal of Animal Physiology and Animal Nutrition 104, 1454-1461.
| Crossref | Google Scholar | PubMed |
