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RESEARCH ARTICLE

Effect of feeding forage or concentrate starter diets in early life on life-time growth, carcass traits and meat quality of Wagyu × Friesian cattle

V. T. Burggraaf https://orcid.org/0000-0001-7592-7204 A , C. R. Craigie A B , M. A. Khan C , P. D. Muir D , B. C. Thomson D , K. A. Lowe C , S. R. Leath A , K. R. Taukiri A , M. Staincliffe A and S. A. McCoard C E
+ Author Affiliations
- Author Affiliations

A AgResearch Ltd, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand.

B AgResearch Ltd, Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New Zealand.

C AgResearch Ltd, Grasslands Research Centre, Private Bag 11008, Palmerston North 4474, New Zealand.

D On-Farm Research, PO Box 1142, Hastings 4156, New Zealand.

E Corresponding author. Email: sue.mccoard@agresearch.co.nz

Animal Production Science 60(15) 1850-1860 https://doi.org/10.1071/AN19486
Submitted: 26 August 2019  Accepted: 31 March 2020   Published: 20 July 2020

Abstract

Context: Nutrition in early life can affect the long-term performance of livestock.

Aims: The present study evaluated the effects of rearing Wagyu × Holstein Friesian heifer calves either on a concentrate pelleted starter (CS; 90% DM, 19.3% crude protein (CP), and 13.8 MJ metabolisable energy (ME) per kilogram DM) or a forage starter (FS, ensiled alfalfa, Medicago sativa; 45% DM, 18.5% CP and 9.7 MJ metabolisable energy per kilogram DM) on lifetime growth, blood metabolites, carcass traits and meat quality.

Methods: Calves (n = 60) were allocated either to CS or FS diets. All calves received their solid feeds ad libitum from Week 1 and were fed milk in group pens (n = 10/pen) at 4 L/calf.day until Week 7, then 2 L/calf.day for 2 weeks. Calves were transferred to pasture 1 week after milk removal, with starter feeds removed by Week 15. All heifers were then managed as one group on a pasture-based diet until slaughter at 26–30 months of age (average of 483 kg liveweight).

Key results: Calves offered the CS diet were 14 kg heavier than were calves on the FS diet at 15 weeks (P < 0.001). However, liveweight was similar between the groups by the time of slaughter (P > 0.1). All blood parameters studied were similar (P > 0.05) between the treatments at 12 months and at slaughter. Depth of eye muscle and subcutaneous fat, measured using ultrasonography before slaughter, carcass weight and meat pH, marbling, shear force, moisture loss during cooking, total fat and fat and meat colour were also similar between the treatments (P > 0.05). Some potential was found for using blood markers to predict carcass traits and meat quality.

Conclusions: Solid feed type during rearing had little effect on liveweight or meat characteristics from 12 months of age to slaughter of Wagyu × Friesian heifers.

Implications: Calves can be reared on FS diets with similar long-term production performance as on CS diets.

Additional keywords: crossbred calves, economics, solid feeds.


References

Abeni F, Calamari L, Stefanini L, Pirlo G (2012) Effect of average daily gain on body size, metabolism, and milk production of Italian Holstein heifers raised on two different planes of nutrition and calving at two different ages. Livestock Science 149, 7–17.
Effect of average daily gain on body size, metabolism, and milk production of Italian Holstein heifers raised on two different planes of nutrition and calving at two different ages.Crossref | GoogleScholarGoogle Scholar |

AOAC (2012) Official method 960.39. Fat (crude) or ether extract in meat. In ‘Official methods of analysis of AOAC International’. 19th edn. (AOAC International: Gaithersburg, MD, USA)

AUS-MEAT (2018) ‘Handbook of Australian beef processing. Version 4. 17/04/2018.’ Available at https://www.ausmeat.com.au/WebDocuments/Producer_HAP_Beef_Small.pdf.[Verified 30 May 2018]

Bach A (2012) Ruminant nutrition symposium: optimizing performance of the offspring: nourishing and managing the dam and postnatal calf for optimal lactation, reproduction and immunity. Journal of Animal Science 90, 1835–1845.
Ruminant nutrition symposium: optimizing performance of the offspring: nourishing and managing the dam and postnatal calf for optimal lactation, reproduction and immunity.Crossref | GoogleScholarGoogle Scholar | 21926322PubMed |

Bach A, Ahedo J (2008) Record keeping and economics of dairy heifers: dairy heifer management. The Veterinary Clinics of North America. Food Animal Practice 24, 117–138.
Record keeping and economics of dairy heifers: dairy heifer management.Crossref | GoogleScholarGoogle Scholar | 18299035PubMed |

Berge P (1991) Long-term effects of feeding during calfhood on subsequent performance in beef cattle (a review). Livestock Production Science 28, 179–201.
Long-term effects of feeding during calfhood on subsequent performance in beef cattle (a review).Crossref | GoogleScholarGoogle Scholar |

Bermingham EN, Gomes Reis M, Subbaraj AK, Cameron-Smith D, Fraser K, Jonker A, Craigie CR (2018) Distribution of fatty acids and phospholipids in different table cuts and co-products from New Zealand pasture-fed Wagyu–dairy cross beef cattle. Meat Science 140, 26–37.
Distribution of fatty acids and phospholipids in different table cuts and co-products from New Zealand pasture-fed Wagyu–dairy cross beef cattle.Crossref | GoogleScholarGoogle Scholar | 29501930PubMed |

Bindon BM (2004) A review of genetic and non-genetic opportunities for manipulation of marbling. Australian Journal of Experimental Agriculture 44, 687–696.
A review of genetic and non-genetic opportunities for manipulation of marbling.Crossref | GoogleScholarGoogle Scholar |

Blanco M, Villalba D, Rippoll G, Sauerwein HI, Casasus I (2009) Effects of early weaning and breed on calf performance and carcass and meat quality in autumn-born bull calves weaned at 90 or 150 days of age. Animal 2, 779–789.

Brickell JS, McGowan MM, Wathes DC (2009) Effect of management factors and blood metabolites during the rearing period on growth in dairy heifers on UK farms. Domestic Animal Endocrinology 36, 67–81.
Effect of management factors and blood metabolites during the rearing period on growth in dairy heifers on UK farms.Crossref | GoogleScholarGoogle Scholar | 19059748PubMed |

Cano-Muñoz G (1991) ‘Manual on meat cold store operation and management.’ (Food and Agriculture Organization of the United Nations: Rome) Available at http://www.fao.org/docrep/004/T0098E/T0098E02.htm.[Verified 31 May 2018]

Carrillo JA, He Y, Li Y, Liu J, Erdman RA, Sonstegard TS, Song J (2016) Integrated metabolomic and transcriptome analyses reveal finishing forage affects metabolic pathways related to beef quality and animal welfare. Scientific Reports 6, 25948
Integrated metabolomic and transcriptome analyses reveal finishing forage affects metabolic pathways related to beef quality and animal welfare.Crossref | GoogleScholarGoogle Scholar | 27185157PubMed |

Cassar-Malek I, Picard B (2016) Expression marker-based strategy to improve beef quality. The Scientific World Journal 2016, 2185323
Expression marker-based strategy to improve beef quality.Crossref | GoogleScholarGoogle Scholar | 27066527PubMed |

Castells L, Bach A, Aris A, Terré M (2013) Effects of forage provision to young calves on rumen fermentation and development of the gastrointestinal tract. Journal of Dairy Science 96, 5226–5236.
Effects of forage provision to young calves on rumen fermentation and development of the gastrointestinal tract.Crossref | GoogleScholarGoogle Scholar | 23706491PubMed |

Chilliard Y (1999) Metabolic adaptations and nutrient partitioning in the lactating animal. In ‘Biology of lactation’. (Eds J Martinet, LM Houdebine, HH Head) pp. 503–552. (INRA: Paris)

Chrystall NB, Devine CE (1991) ‘Quality assurance for tenderness.’ Publication, No. 872. (Meat Industries Research Institute of New Zealand: Hamilton, New Zealand)

Chung CS, Cho WK, Jang IS, Lee SS, Moon YH (2017) Effects of feeding system on growth performance, plasma biochemical components and hormones, and carcass characteristics in Hanwoo steers. Asian-Australasian Journal of Animal Sciences 30, 1117–1123.
Effects of feeding system on growth performance, plasma biochemical components and hormones, and carcass characteristics in Hanwoo steers.Crossref | GoogleScholarGoogle Scholar | 28669143PubMed |

Corson DC, Waghorn GC, Ulyatt MJ, Lee J (1999) NIRS: forage analysis and livestock feeding. Proceedings of the New Zealand Grassland Association 61, 127–132.

Daly CC, Young OA, Graafhuis AE, Moorhead SM, Easton HS (1999) Some effects of diet on beef meat and fat attributes. New Zealand Journal of Agricultural Research 42, 279–287.
Some effects of diet on beef meat and fat attributes.Crossref | GoogleScholarGoogle Scholar |

Dodson MV, Jiang Z, Chen J, Hausman GJ, Guan LL, Novakofski J, Thompson DP, Lorenzen CL, Fernyhough ME, Mir PS, Reecy JM (2010) Allied industry approaches to alter intramuscular fat content and composition in beef animals. Journal of Food Science 75, R1–R8.
Allied industry approaches to alter intramuscular fat content and composition in beef animals.Crossref | GoogleScholarGoogle Scholar | 20492190PubMed |

Du M, Tong J, Zhao J, Underwood KR, Zhu M, Ford SP, Nathanielsz PW (2010) Fetal programming of skeletal muscle development in ruminant animals. Journal of Animal Science 88, E51–E60.
Fetal programming of skeletal muscle development in ruminant animals.Crossref | GoogleScholarGoogle Scholar | 19717774PubMed |

Du M, Wang B, Fu X, Yang Q, Zhu MJ (2015) Fetal programming in meat production. Meat Science 109, 40–47.
Fetal programming in meat production.Crossref | GoogleScholarGoogle Scholar | 25953215PubMed |

Elsasser TH, Rumsey TS, Hammon HM (1989) Influence of diet on basal and growth hormone-stimulated plasma concentrations of IGF-1 in beef cattle. Journal of Animal Science 67, 128–141.
Influence of diet on basal and growth hormone-stimulated plasma concentrations of IGF-1 in beef cattle.Crossref | GoogleScholarGoogle Scholar | 2925537PubMed |

Enríquez DH, Ungerfeld R, Quintans G, Guidoni AL, Hötzel MJ (2010) The effects of alternative weaning methods on behaviour in beef calves. Livestock Science 128, 20–27.
The effects of alternative weaning methods on behaviour in beef calves.Crossref | GoogleScholarGoogle Scholar |

Farmer LJ, Farrell DT (2018) Review: beef-eating quality: a European journey. Animal 12, 2424–2433.
Review: beef-eating quality: a European journey.Crossref | GoogleScholarGoogle Scholar | 30004320PubMed |

Geary TW, McFadin EL, MacNeil MD, Grings EE, Short RE, Funston RN, Keisler DH (2003) Leptin as a predictor of carcass composition in beef cattle. Journal of Animal Science 81, 1–18.
Leptin as a predictor of carcass composition in beef cattle.Crossref | GoogleScholarGoogle Scholar | 12597366PubMed |

Goll DE, Thompson VF, Taylor RG, Ouali A (1998) The calpain system and skeletal muscle growth. Canadian Journal of Animal Science 78, 503–512.
The calpain system and skeletal muscle growth.Crossref | GoogleScholarGoogle Scholar |

Greenwood PL, Café LM (2007) Prenatal and pre-weaning growth and nutrition of cattle: longterm consequences for beef production. Animal 1, 1283–1296.
Prenatal and pre-weaning growth and nutrition of cattle: longterm consequences for beef production.Crossref | GoogleScholarGoogle Scholar | 22444884PubMed |

Greenwood PL, Café LM, Hearnshaw H, Hennessy DW (2005) Consequences of nutrition and growth retardation early in life for growth and composition of cattle and eating quality of beef. Recent Advances in Animal Nutrition in Australia 15, 183–195.

Greenwood PL, Café LM, Hearnshaw H, Hennessy DW, Thompson JM, Morris SG (2006) Long-term consequences of birth weight and growth to weaning for carcass, yield and beef quality characteristics of Piedmontese and Wagyu-sired cattle. Australian Journal of Experimental Agriculture 46, 257–269.
Long-term consequences of birth weight and growth to weaning for carcass, yield and beef quality characteristics of Piedmontese and Wagyu-sired cattle.Crossref | GoogleScholarGoogle Scholar |

Hadorn U, Hammon H, Bruckmaier RM, Blum JM (1997) Delayed colostrum intake by one day has important effects on metabolic traits and on gastrointestinal and metabolic hormones ion neonatal caves. The Journal of Nutrition 127, 2011–2023.
Delayed colostrum intake by one day has important effects on metabolic traits and on gastrointestinal and metabolic hormones ion neonatal caves.Crossref | GoogleScholarGoogle Scholar | 9311959PubMed |

Haisan J, Oba M, Ambrose DJ, Steele MA (2018) Short communication: the effects of offering a high or low plane of milk preweaning on insulin-like growth factor and insulin-like growth factor binding proteins in dairy heifer calves. Journal of Dairy Science 101, 11441–11446.
Short communication: the effects of offering a high or low plane of milk preweaning on insulin-like growth factor and insulin-like growth factor binding proteins in dairy heifer calves.Crossref | GoogleScholarGoogle Scholar | 30268627PubMed |

Hausman GJ, Dodson MV, Ajuwon K, Azain M, Barnes KM, Guan LL, Jiang Z, Poulos SP, Sainz RD, Smith S, Spurlock M, Novakofski J, Fernyhough ME, Bergen WG (2009) Board invited review: the biology and regulation of preadipocytes and adipocytes in meat animals. Journal of Animal Science 87, 1218–1246.
Board invited review: the biology and regulation of preadipocytes and adipocytes in meat animals.Crossref | GoogleScholarGoogle Scholar | 18849378PubMed |

Hearnshaw H (1997) Effect of pre-weaning nutrition on post-weaning growth, carcase and meat quality traits. In ‘Growth and development of cattle. Proceedings of the growth and development workshop’. (Eds DW Hennessy, SR McLennan, VH Oddy) pp. 59–67. (Cooperative Research Centre for Cattle and Beef Quality: Armidale, NSW, Australia)

Heinrichs AJ, Heinrichs BS (2011) A prospective study of calf factors affecting first-lactation and lifetime milk production and age of cows when removed from the herd. Journal of Dairy Science 94, 336–341.
A prospective study of calf factors affecting first-lactation and lifetime milk production and age of cows when removed from the herd.Crossref | GoogleScholarGoogle Scholar | 21183043PubMed |

Hennessy DW, Morris SG, Allingham PG (2001) Improving the pre-weaning nutrition of calves by supplementation of the cow and/or the calf while grazing low quality pastures. 2. Calf growth, carcass yield and eating quality. Australian Journal of Experimental Agriculture 41, 715–724.
Improving the pre-weaning nutrition of calves by supplementation of the cow and/or the calf while grazing low quality pastures. 2. Calf growth, carcass yield and eating quality.Crossref | GoogleScholarGoogle Scholar |

Keady SM, Waters SM, Hamill RM, Dunne PG, Keane MG, Richardson RI, Kenny DA, Moloney AP (2017) Compensatory growth in crossbred Aberdeen Angus and Belgian Blue steers: effects on the colour, shear force and sensory characteristics of longissimus muscle. Meat Science 125, 128–136.
Compensatory growth in crossbred Aberdeen Angus and Belgian Blue steers: effects on the colour, shear force and sensory characteristics of longissimus muscle.Crossref | GoogleScholarGoogle Scholar | 27951464PubMed |

Kesser J, Korst M, Koch C, Romberg FJ, Rehage J, Muller U, Schmicke M, Eder K, Hammon HM, Sadri H, Sauerwein H (2017) Different milk feeding intensities during the first 4 weeks of rearing dairy calves: part 2: effects on the metabolic and endocrine status during calfhood around the first lactation. Journal of Dairy Science 100, 3109–3125.
Different milk feeding intensities during the first 4 weeks of rearing dairy calves: part 2: effects on the metabolic and endocrine status during calfhood around the first lactation.Crossref | GoogleScholarGoogle Scholar | 28131581PubMed |

Khan MA, Weary DM, von Keyserlingk MAG (2011) Invited review: effects of milk ration on solid feed intake, weaning and performance in dairy heifers. Journal of Dairy Science 94, 1071–1081.
Invited review: effects of milk ration on solid feed intake, weaning and performance in dairy heifers.Crossref | GoogleScholarGoogle Scholar | 21338773PubMed |

Khan MA, Bach A, Weary DM, von Keyserlingk MAG (2016) Invited review: transitioning from milk to solid feed in dairy heifers. Journal of Dairy Science 99, 885–902.
Invited review: transitioning from milk to solid feed in dairy heifers.Crossref | GoogleScholarGoogle Scholar | 26709160PubMed |

Khan MA, Burggraaf VT, Thomson B, Muir P, Lowe J, Koolaard J, Heiser A, Leath S, McCoard S (2020) Effect of feeding forage or concentrate starter early in life on rumen fermentation, metabolic response, immune functions, and growth of Wagyu × Friesian calves to 10 months of age. Animal Production Science.
Effect of feeding forage or concentrate starter early in life on rumen fermentation, metabolic response, immune functions, and growth of Wagyu × Friesian calves to 10 months of age.Crossref | GoogleScholarGoogle Scholar |

Koohmaraie M, Kent MP, Shackelford SD, Veiseth E, Wheeler TL (2002) Meat tenderness and muscle growth: is there any relationship? Meat Science 62, 345–352.
Meat tenderness and muscle growth: is there any relationship?Crossref | GoogleScholarGoogle Scholar | 22061610PubMed |

Li X, Ekerljung M, Lundstrom K, Lunden A (2013) Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden. Meat Science 94, 153–158.
Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden.Crossref | GoogleScholarGoogle Scholar | 23501244PubMed |

Loudon DMW, Tarr G, Lean IJ, Polkinghorne R, McGilchrist P, Dunshea FR, Gardner GE, Pethick DW (2019) The impact of pre-slaughter stress on beef eating quality. Animals 9, 612
The impact of pre-slaughter stress on beef eating quality.Crossref | GoogleScholarGoogle Scholar |

Lucas A (1991) Programming by early nutrition in man. Ciba Foundation Symposium 156, 38–50.

Lunt DK, Riley RR, Smith SB (1993) Growth and carcass characteristics of Angus and American Wagyu steers. Meat Science 34, 327–334.
Growth and carcass characteristics of Angus and American Wagyu steers.Crossref | GoogleScholarGoogle Scholar | 22060741PubMed |

MacFarlane PG, Marer JM (1966) An apparatus for determining the tenderness of meat. Food Technology 20, 134–135.

McCoard S, Heiser A, Lowe K, Molenaar A, MacLean P, Johnstone P, Leath S, Hoskin SO, Khan MA (2019) Effect of weaning age on growth, mammary gland development, and immune function in Holstein Friesian calves fed conserved alfalfa (FiberStart). Journal of Dairy Science 102, 6076–6087.
Effect of weaning age on growth, mammary gland development, and immune function in Holstein Friesian calves fed conserved alfalfa (FiberStart).Crossref | GoogleScholarGoogle Scholar | 31079903PubMed |

Morris ST (2013) The New Zealand beef cattle industry. Proceedings of the New Zealand Society of Animal Production 73, 1–4.

Muir PD, Nieuwenhuis G, Smith NB, Ormond AWA (2000) A comparison of rearing systems for dairy beef calves. Proceedings of the New Zealand Grassland Association 62, 9–11.

Muir PD, Fugle CJ, Smith NB (2003) Calf rearing using a once-a-day milk feeding system: what can the dairy industry learn from the bull beef industry. In ‘Proceedings of the South Island dairy event’. (Ed. D Kilgour) pp. 226–234. (Caxton Press: Christchurch, New Zealand)

Polkinghorne R, Philpott J, Thompson J (2018) Do extended transport times and rest periods impact on eating quality of beef carcasses? Meat Science 140, 101–111.
Do extended transport times and rest periods impact on eating quality of beef carcasses?Crossref | GoogleScholarGoogle Scholar | 29549843PubMed |

Prokop L, Kaske M, Maccari P, Lucius R, Kunz HJ, Wiedemann S (2015) Intensive rearing of male calves during the first three weeks of life has long-term effects on number of isltets of Langerhans and insulin stained area in the pancreas. Journal of Animal Science 93, 988–998.
Intensive rearing of male calves during the first three weeks of life has long-term effects on number of isltets of Langerhans and insulin stained area in the pancreas.Crossref | GoogleScholarGoogle Scholar | 26020877PubMed |

R Core Team (2016) ‘R: a language and environment for statistical computing.’ (R Foundation for Statistical Computing: Vienna) Available at http://www.R-project.org/ [Verified 14 May 2020]

Reardon TF, Everitt GC (1972) Effects of pre-weaning nutrition on subsequent growth rate, feed conversion efficiency and carcass composition of identical twin steers. Proceedings of the New Zealand Society of Animal Production 32, 26–38.

Reddy KE, Jeong J, Baek YC, Oh K, Kim M, So KM, Kim MJ, Kim DW, Park SK, Lee HJ (2017) Early weaning of calves after different dietary regimens affects later rumen development, growth, and carcass traits in Hanwoo cattle. Asian-Australasian Journal of Animal Sciences 30, 1425–1434.
Early weaning of calves after different dietary regimens affects later rumen development, growth, and carcass traits in Hanwoo cattle.Crossref | GoogleScholarGoogle Scholar | 28728406PubMed |

Robinson AM, Williamson DH (1980) Physiological roles of ketone bodies as substrates and signals in mammalian tissues. Physiological Reviews 60, 143–187.
Physiological roles of ketone bodies as substrates and signals in mammalian tissues.Crossref | GoogleScholarGoogle Scholar | 6986618PubMed |

Stewart SM, McGilchrist P, Gardner GE, Pethick DW (2018) Asociation between loin ultimate pH and plasma indicators of pre-slaughter stressors in Australian Lamb 1. Meat and Muscle Biology 2, 254–264.
Asociation between loin ultimate pH and plasma indicators of pre-slaughter stressors in Australian Lamb 1.Crossref | GoogleScholarGoogle Scholar |

Therkildsen M, Houbak MB, Byrne DV (2008) Feeding strategy for improving tenderness has opposite effects in two different muscles. Meat Science 80, 1037–1045.
Feeding strategy for improving tenderness has opposite effects in two different muscles.Crossref | GoogleScholarGoogle Scholar | 22063834PubMed |

Tornberg E (2005) Effects of heat on meat proteins: implications on structure and quality of meat products. Meat Science 70, 493–508.
Effects of heat on meat proteins: implications on structure and quality of meat products.Crossref | GoogleScholarGoogle Scholar | 22063748PubMed |

van der Walt JG, Hattingh J, Petty DB, Grobler MJ, Ganhao MF (1993) Effect of a 72 hour fast on physiological stress indicators in feedlot cattle. Journal of the South African Veterinary Association 64, 39–42.

Venkata Reddy B, Sivakumar AS, Jeong DW, Woo YB, Park SJ, Lee SY, Byun JY, Kim CH, Cho SH, Hwang I (2015) Beef quality traits of heifer in comparison with steer, bull and cow at various feeding environments. Animal Science Journal 86, 1–16.
Beef quality traits of heifer in comparison with steer, bull and cow at various feeding environments.Crossref | GoogleScholarGoogle Scholar | 25236779PubMed |

Warner R, Greenwood P, Pethick D, Ferguson D (2010) Genetic and environmental effects on meat quality. Meat Science 86, 171–183.
Genetic and environmental effects on meat quality.Crossref | GoogleScholarGoogle Scholar | 20561754PubMed |

Watanabe G, Motoyama M, Nakajima I, Sasaki K (2018) Relationship between water-holding capacity and intramuscular fat content in Japanese commercial pork loin. Asian–Australasian Journal of Animal Sciences 31, 914–918.
Relationship between water-holding capacity and intramuscular fat content in Japanese commercial pork loin.Crossref | GoogleScholarGoogle Scholar | 29268572PubMed |

Yelich JV, Wettemann RP, Marston TT, Spicer LJ (1996) Lutenizing hormone, growth hormone, insulin-like growth factor-I, insulin and metabolites before puberty in heifers fed to gain at two rates. Domestic Animal Endocrinology 13, 325–338.
Lutenizing hormone, growth hormone, insulin-like growth factor-I, insulin and metabolites before puberty in heifers fed to gain at two rates.Crossref | GoogleScholarGoogle Scholar | 8839626PubMed |

Yonekura S, Oka A, Noda M, Uozumi N, Yonezawa T, Katoh K, Obara Y (2002) Relationship between serum leptin concentrations and the marbling scores in Japanese Black Cattle. Animal Science Journal 73, 51–57.
Relationship between serum leptin concentrations and the marbling scores in Japanese Black Cattle.Crossref | GoogleScholarGoogle Scholar |