Dietary nitrogen utilisation and prediction of amino acid requirements in equids
Nathalie L. Trottier A C and Luis O. Tedeschi B
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, Michigan State University, 474 South. Shaw Lane, 2209 Anthony Hall, East Lansing, MI 48824, USA.
B Department of Animal Science, Texas A& M University, Room 133 Kleberg, 2471 TAMU, College Station, TX 77843, USA.
C Corresponding author. Email: trottier@msu.edu
Animal Production Science 59(11) 2057-2068 https://doi.org/10.1071/AN19304
Submitted: 23 May 2019 Accepted: 12 July 2019 Published: 17 September 2019
Abstract
The equine population represents an important sector of animal agriculture and, thus, contributes to environmental contamination. The horse industry lags behind other livestock industries in developing prediction models to estimate N and amino acid (AA) requirements aimed at precision feeding and management to optimise animal health and performance while mitigating nutrient excretion. Effective predictions of N utilisation and excretion are based on knowledge of ingredient protein quality and the determinants of N and AA requirements. Protein quality is evaluated on the basis of N and AA digestibility and AA composition. Amino acid composition of grains, pulses and oil seeds is extensive, but there is large deficit on that of forages. Several studies have reported on pre- and post-caecal N digestibility in horses, demonstrating that a large proportion of N from forages is metabolised post-caecally. Few have reported on AA digestibility. It is proposed that whole-tract (i.e. faecal) N and AA digestibility be used in evaluating feed-ingredient protein quality in equids to begin designing predictive models of N and AA requirements. Nitrogen gain and AA composition in deposited tissues and their corresponding efficiency of utilisation are the key determinants for a prediction model. We estimated that N utilisation for maintenance is 0.74. Maintenance requirements for N and AA were derived from faecal N and AA losses in horses and expressed as a function of dry-matter intake and from integument losses in swine. Relative to our factorial model, the NRC (2007) requirement for lysine and N is overestimated when based on a segmented curve and a breakpoint. When based on N equilibrium, lysine NRC (2007) requirement estimate agrees with our factorial model estimate, while N requirement is underestimated. The pool of AA profile used to express requirements of other essential AA has a large impact on requirement, as shown, in particular, for threonine. Threonine requirement based on faecal endogenous AA profile is higher than is lysine requirement for maintenance and lactation.
Additional keywords: efficiency, excretion, factorial approach, horses, modelling, protein.
References
Badiani A, Nanni N, Gatta P, Tolomelli B, Manfredini M (1997) Nutrient profile of horsemeat. Journal of Food Composition and Analysis 10, 254–269.| Nutrient profile of horsemeat.Crossref | GoogleScholarGoogle Scholar |
Bott RC, Greene EA, Trottier NL, Williams CA, Westendorf ML, Swinker AM, Mastellar SL, Martinson K (2016) Environmental implications of nitrogen output on horse operations: a review. Journal of Equine Veterinary Science 45, 98–106.
| Environmental implications of nitrogen output on horse operations: a review.Crossref | GoogleScholarGoogle Scholar |
Bryden WL (1991) Amino acid requirements of horses estimated from tissue composition. In ‘Proceedings of the Nutrition Society of Australia, 16’. (Ed. The Society) p. 53. (HEC Press: Melbourne)
Coenen M, Kienzle E, Vervuert I, Zeyner A (2011) Recent German developments in the formulation of energy and nutrient requirements in horses and the resulting feeding recommendations. Journal of Equine Veterinary Science 31, 219–229.
| Recent German developments in the formulation of energy and nutrient requirements in horses and the resulting feeding recommendations.Crossref | GoogleScholarGoogle Scholar |
Csapó J, Stefler J, Makray S, Csapó-Kiss Zs (1995) Composition of mares’ colostrum and milk. Fat content, fatty acid composition and vitamin content. International Dairy Journal 5, 393–402.
| Composition of mares’ colostrum and milk. Fat content, fatty acid composition and vitamin content.Crossref | GoogleScholarGoogle Scholar |
Davis TA, Nguyen HV, Garcia-Bravo R, Fiorotto ML, Jackson EM, Lewis DS, Lee DR, Reeds PJ (1994) Amino acid composition of human milk is not unique. The Journal of Nutrition 124, 1126–1132.
| Amino acid composition of human milk is not unique.Crossref | GoogleScholarGoogle Scholar | 8027865PubMed |
DePew CL, Thompson DL, Fernandez JM, Sticker LS, Burleigh DW (1994) Changes in concentrations of hormones, metabolites, and amino acids in plasma of adult horses relative to overnight feed deprivation followed by a pellet-hay meal fed at noon. Journal of Animal Science 72, 1530–1539.
| Changes in concentrations of hormones, metabolites, and amino acids in plasma of adult horses relative to overnight feed deprivation followed by a pellet-hay meal fed at noon.Crossref | GoogleScholarGoogle Scholar | 8071178PubMed |
Ellis A (2004) The Dutch net protein system. In ‘Nutrition of the performance horse’. EAAP publication no. 111. (Eds V Juliand, W Martin-Rosset) pp. 141–143. (Wageningen Academic Publishers: Wageningen, The Netherlands)
Fan MZ, Sauer WC (1995) Determination of apparent ileal amino acid digestibility in barley and canola meal for pigs with the direct, difference, and regression methods. Journal of Animal Science 73, 2364–2374.
| Determination of apparent ileal amino acid digestibility in barley and canola meal for pigs with the direct, difference, and regression methods.Crossref | GoogleScholarGoogle Scholar | 8567474PubMed |
Fisher H, Scott H (1954) The essential amino acid requirements of chicks as related to their proportional occurrence in the fat free carcass. Archives of Biochemistry and Biophysics 51, 517–519.
| The essential amino acid requirements of chicks as related to their proportional occurrence in the fat free carcass.Crossref | GoogleScholarGoogle Scholar | 13189594PubMed |
Food and Agriculture Organization of the United Nations (FAO) (2013) Dietary protein quality evaluation in human nutrition. Report of an FAO Expert Consultation. FAO Food and Nutrition Paper. No. 92. FAO, Rome.
Franco D, Crecente S, Vázquez JA, Gómez M, Lorenzo JM (2013) Effect of cross breeding and amount of finishing diet on growth parameters, carcass and meat composition of foals slaughtered at 15 months of age. Meat Science 93, 547–556.
| Effect of cross breeding and amount of finishing diet on growth parameters, carcass and meat composition of foals slaughtered at 15 months of age.Crossref | GoogleScholarGoogle Scholar | 23273463PubMed |
Gibbs P, Potter G, Schelling G, Kreider J, Boyd C (1988) Digestion of hay protein in different segments of the equine digestive tract. Journal of Animal Science 66, 400–406.
| Digestion of hay protein in different segments of the equine digestive tract.Crossref | GoogleScholarGoogle Scholar | 3372383PubMed |
Glade M (1983) Nutrition and performance of racing thoroughbreds. Equine Veterinary Journal 15, 31–36.
| Nutrition and performance of racing thoroughbreds.Crossref | GoogleScholarGoogle Scholar |
Graham P, Brendemuhl L, TenBroack S (1994) The effect of supplemental lysine and threonine on growth and development of yearling horses. Journal of Animal Science 72, 380–386.
| The effect of supplemental lysine and threonine on growth and development of yearling horses.Crossref | GoogleScholarGoogle Scholar | 8157522PubMed |
Harper M, Swinker A, Staniar W, Welker A (2009) Ration evaluation of Chesapeake Bay watershed horse farms from a nutrient management perspective. Journal of Equine Veterinary Science 29, 401–402.
| Ration evaluation of Chesapeake Bay watershed horse farms from a nutrient management perspective.Crossref | GoogleScholarGoogle Scholar |
Heger P, Patráš S, Nitrayová J, Karcol P, Dolešová P (2008) Lysine maintenance requirement and efficiency of its utilisation in young pigs as estimated by comparative slaughter technique. Archives of Animal Nutrition 62, 182–192.
| Lysine maintenance requirement and efficiency of its utilisation in young pigs as estimated by comparative slaughter technique.Crossref | GoogleScholarGoogle Scholar |
Kern D, Slyter L, Weaver J, Leffel E, Samuelsons G (1973) Pony cecum vs. steer rumen: the effect of oats and hay on the microbial ecosystem. Journal of Animal Science 37, 463–469.
| Pony cecum vs. steer rumen: the effect of oats and hay on the microbial ecosystem.Crossref | GoogleScholarGoogle Scholar | 4201259PubMed |
Kienzle E, Fehrle S, Opitz B (2002) Interactions between the apparent energy and nutrient digestibilities of a concentrate mixture and roughages in horses. The Journal of Nutrition 132, 1778S–1780S.
| Interactions between the apparent energy and nutrient digestibilities of a concentrate mixture and roughages in horses.Crossref | GoogleScholarGoogle Scholar | 12042526PubMed |
Lorenzo JM, Pateiro M (2013) Influence of type of muscles on nutritional value of foal meat. Meat Science 93, 630–638.
| Influence of type of muscles on nutritional value of foal meat.Crossref | GoogleScholarGoogle Scholar | 23273474PubMed |
Martin-Rosset W, Tisserand J (2004) Evaluation and expression of protein allowances and protein value of feeds in the MADC system for the performance horse. In ‘Nutrition of the performance horse: which system in Europe for evaluating the nutritional requirements?’. (Eds V Juliand, W Martin-Rosset) pp. 103–140. (Wageningen Academic Publishers: Wageningen, The Netherlands)
Moughan PJ (1999) Protein metabolism in the growing pig. In ‘Quantitative biology of the pig’. (Ed. I Kyriazakis) pp. 299–331. (CABI: Wallingford, UK)
Nelson D, Tyznik W (1971) Protein and nonprotein nitrogen utilization in the horse. Journal of Animal Science 32, 68–73.
| Protein and nonprotein nitrogen utilization in the horse.Crossref | GoogleScholarGoogle Scholar | 5546878PubMed |
National Research Council (NRC) (2007) ‘Nutrient requirements of horses.’ 6th revised edn. (National Research Council of the National Academies: Washington, DC, USA)
National Research Council (NRC) (2012) ‘Nutrient requirements of swine.’ 11th revised edn. (National Research Council of the National Academies: Washington, DC, USA)
Palmgren Karlsson C, Lindberg J, Rundgren M (2000) Associative effects on total tract digestibility in horses fed different ratios of grass hay and whole oats. Livestock Production Science 65, 143–153.
| Associative effects on total tract digestibility in horses fed different ratios of grass hay and whole oats.Crossref | GoogleScholarGoogle Scholar |
Platt H (1978) Growth and maturity in the equine fetus. Journal of the Royal Society of Medicine 71, 658–661.
| Growth and maturity in the equine fetus.Crossref | GoogleScholarGoogle Scholar | 712744PubMed |
Polidori P, Cavallucci C, Beghelli D, Vincenzetti S (2009) Physical and chemical characteristics of donkey meat from Martina Franca breed. Meat Science 82, 469–471.
| Physical and chemical characteristics of donkey meat from Martina Franca breed.Crossref | GoogleScholarGoogle Scholar | 20416677PubMed |
Potter G, Huchton J (1975) Growth of yearling horses fed different sources of protein with supplemental lysine. In ‘Proceedings of the 4th equine nutrition and physiology symposium’, 16–18 January, Pomona California. pp. 19–20.
Ralston SL (1984) Control of feeding in horses. Journal of Animal Science 59, 1354–1361.
| Control of feeding in horses.Crossref | GoogleScholarGoogle Scholar | 6392275PubMed |
Reitnour CM, Salsbury RL (1972) Digestion and utilization of cecally infused protein by the equine. Journal of Animal Science 35, 1190–1193.
| Digestion and utilization of cecally infused protein by the equine.Crossref | GoogleScholarGoogle Scholar | 4678724PubMed |
Reitnour C, Treece J (1971) Relationship of nitrogen source to certain blood components and nitrogen balance in the equine. Journal of Animal Science 32, 487–490.
| Relationship of nitrogen source to certain blood components and nitrogen balance in the equine.Crossref | GoogleScholarGoogle Scholar | 5102194PubMed |
Reitnour CM, Baker JP, Mitchell GE, Little CO (1969) Nitrogen digestion in different segments of the equine digestive tract. Journal of Animal Science 29, 332–334.
| Nitrogen digestion in different segments of the equine digestive tract.Crossref | GoogleScholarGoogle Scholar | 5391280PubMed |
Ringel J, Susenbeth A (2009) Lysine requirement for maintenance in growing pigs. Livestock Science 120, 144–150.
| Lysine requirement for maintenance in growing pigs.Crossref | GoogleScholarGoogle Scholar |
Rotz C (2004) Management to reduce nitrogen losses in animal production. Journal of Animal Science 82, 119–137.
Seong P, Kyoung M, Kang G, Cho S, Park B, Chae H, Ba H (2016) The differences in chemical composition, physical quality traits and nutritional values of horse meat as affected by various retail cut types. Asian-Australasian Journal of Animal Sciences 29, 89–99.
| The differences in chemical composition, physical quality traits and nutritional values of horse meat as affected by various retail cut types.Crossref | GoogleScholarGoogle Scholar | 26732332PubMed |
Slade LM, Bishop R, Morris JG, Robinson DW (1971) Digestion and absorption of 15N-labelled microbial protein in the large intestine of the horse. The British Veterinary Journal 127, xi–xiii.
| Digestion and absorption of 15N-labelled microbial protein in the large intestine of the horse.Crossref | GoogleScholarGoogle Scholar |
Stein HH, Fuller MF, Moughan PJ, Sève B (2007) Definition of apparent, true, and standardized ileal digestibility of amino acids in pigs. Livestock Science 109, 282–285.
| Definition of apparent, true, and standardized ileal digestibility of amino acids in pigs.Crossref | GoogleScholarGoogle Scholar |
Stevens C, Hume I (1995) ‘Comparative physiology of the vertebrate digestive system.’ (Cambridge University Press: Cambridge, NY)
Takagi H, Hashimoto Y, Yonemochi C, Ishibashi T, Asai Y, Watanabe R (2003) Digestibility of cereals, oil meals, brans and hays in thoroughbreds. Journal of Equine Science 14, 119–124.
| Digestibility of cereals, oil meals, brans and hays in thoroughbreds.Crossref | GoogleScholarGoogle Scholar |
Tanner S (2014) Evaluating dietary amino acid adequacy in horses using isotopic techniques. PhD Thesis, University of Kentucky, Lexington, KY, USA.
Tanner SL, Wagner AL, Ennis RB, Urschel K (2011) Amino acid concentrations in late lactation mares’ milk. Journal of Equine Veterinary Science 31, 267–268.
| Amino acid concentrations in late lactation mares’ milk.Crossref | GoogleScholarGoogle Scholar |
Trottier NL, Walker R (2015) Nutritional aspects. In ‘Applied food protein chemistry’. (Ed. Z Ustunol) pp. 113–140. (John Wiley & Sons: Oxford, UK)
Trottier NL, Wickens CL (2007) Obligatory and fecal nitrogen losses in adult horses. In ‘Proceedings of the 20th Equine Science Society symposium’, 5–9 June, Hunt Valley, MD, USA. pp. 116–117.
Trottier NL, Bott RC, Woodward A, Greene EA, Williams CA, Westendorf ML, Swinker AM, Mastellar SL, Martinson K (2016) Gastrointestinal nitrogen metabolism of equids and impact on protein requirement. Journal of Equine Veterinary Science 45, 78–86.
| Gastrointestinal nitrogen metabolism of equids and impact on protein requirement.Crossref | GoogleScholarGoogle Scholar |
Van Soest P (1965) Symposium on factors influencing the voluntary intake of herbage by ruminants: voluntary intake in relation to chemical composition and digestibility. Journal of Animal Science 24, 834–843.
| Symposium on factors influencing the voluntary intake of herbage by ruminants: voluntary intake in relation to chemical composition and digestibility.Crossref | GoogleScholarGoogle Scholar |
Van Soest P (1967) Development of a comprehensive system of feed analyses and its application to forages. Journal of Animal Science 26, 119–128.
| Development of a comprehensive system of feed analyses and its application to forages.Crossref | GoogleScholarGoogle Scholar |
Van Soest P (1994) ‘Nutritional ecology of the ruminant.’ (Cornell University Press: Ithaca, NY)
Wickens CL (2003) Dietary protein requirement of mature, moderately exercised horses. MS Thesis, Michigan State University, East Lansing, MI, USA.
Wickens C, Ku PK, Trottier NL (2002) An ideal protein for the lactating mare. Journal of Animal Science 80, 155
Woodward A, Holcombe S, Staniar W, Colvin C, Trottier N (2010a) Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract. Journal of Animal Science 88, 1028–1033.
| Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract.Crossref | GoogleScholarGoogle Scholar | 19933436PubMed |
Woodward AD, Willyard A, Buckley A, Liesman J, de Lange CFM, Trottier NL (2010b) Digestibility of oats in horses using the substitution approach. Journal of Animal Science 88, 202
Woodward A, Nielsen B, Liesman J, Lavin T, Trottier N (2011) Protein quality and utilization of alfalfa, timothy, and oat-supplemented hay in exercised arabian horses. Journal of Animal Science 89, 4081–4092.
| Protein quality and utilization of alfalfa, timothy, and oat-supplemented hay in exercised arabian horses.Crossref | GoogleScholarGoogle Scholar | 21788427PubMed |
Woodward A, Fan M, Geor R, McCutcheon L, Taylor N, Trottier N (2012) Characterization of L-lysine transport across equine and porcine jejunal and colonic brush border membrane. Journal of Animal Science 90, 853–862.
| Characterization of L-lysine transport across equine and porcine jejunal and colonic brush border membrane.Crossref | GoogleScholarGoogle Scholar | 22064742PubMed |
Wootton JF, Argenzio RA (1975) Nitrogen utilization within equine large intestine. The American Journal of Physiology 229, 1062–1067.
| Nitrogen utilization within equine large intestine.Crossref | GoogleScholarGoogle Scholar | 1190319PubMed |
Zeyner A, Kirchhof S, Susenbeth A, Südekum K-H, Kienzle E (2015) A new protein evaluation system for horse feed from literature data. Journal of Nutritional Science 4, e4
| A new protein evaluation system for horse feed from literature data.Crossref | GoogleScholarGoogle Scholar | 26090101PubMed |
