Dietary zinc and growth, carcass characteristics, immune responses, and serum biochemistry of broilers
Shizhen Qin A , Lingyan Zhang B , Fang Ma A , Yanzhuo Che A , Haibo Wang A and Zhaoguo Shi A C
+ Author Affiliations
- Author Affiliations
A Faculty of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou, Gansu Province 730070, P.R. China.
B Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Qingnan Road, Huaxi University Town, Gui'an New District, Guiyang, Guizhou Province 550025, P. R. China.
C Corresponding author. Email: 1991378985@qq.com
Animal Production Science 60(6) 815-822 https://doi.org/10.1071/AN18763
Submitted: 23 December 2018 Accepted: 7 August 2019 Published: 5 March 2020
Abstract
Context: Zinc (Zn) is an essential trace element, and plays an important role in growth, bone formation, feathering and appetite of broilers. Accurate supplementation of this mineral is the aim of the animal husbandry. Thus, it is crucial to optimise the Zn concentration in the diet of broilers.
Aims: The present study was performed to investigate the effects of dietary supplementation of Zn on the growth performance, carcass characteristics, immune responses and serum biochemistry of broilers.
Methods: A total of 180 1-day-old male broilers (Arbor Acres) were randomly allotted by bodyweight to one of five treatments with six replicates of six birds each. The birds were fed a Zn-unsupplemented corn–soybean meal basal diet (27.75 or 26.88 mg/kg Zn by analysis) or one of the four Zn-supplemented diets, which were the basal diet supplemented with 40, 80, 120, or 160 mg Zn /kg as Zn sulfate (reagent grade ZnSO4•7H2O), for 42 days.
Key results: No differences were detected on growth performance or carcass characteristics among treatment groups. However, the total protein concentration and albumin concentration tended (P = 0.09) to increase with an increasing concentration of dietary Zn. The antibody titer of Newcastle disease (ND), and alkaline phosphatase (ALP) in serum on Day 21 were significantly increased (P < 0.05) as the Zn supplementation increased in broiler diets.
Conclusions: These results indicated that dietary Zn supplementation improves the serum antibody titer of ND and ALP activity of broilers, and 86 mg Zn/kg was appropriate for broilers when fed a corn–soybean meal diet in the early stage.
Implications: The present results have provided scientific basis for broiler production, and accurate supplementation of Zn would effectively improve the growth performance and reduce production costs.
Additional keywords: chick, microelement, requirement.
References
Abd El-Wahab A, Radko D, Kamphues J (2013) High dietary levels of biotin and zinc to improve health of foot pads in broilers exposed experimentally to litter with critical moisture content. Poultry Science 92, 1774–1782.| High dietary levels of biotin and zinc to improve health of foot pads in broilers exposed experimentally to litter with critical moisture content.Crossref | GoogleScholarGoogle Scholar | 23776264PubMed |
Abedini M, Shariatmadari F, Torshizi MAK, Ahmadi H (2018) Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late phase of production. Journal of Animal Physiology and Animal Nutrition 102, 736–745.
| Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late phase of production.Crossref | GoogleScholarGoogle Scholar | 29493020PubMed |
Akbaryan M, Mahdavi A, Jebelli-Javan A, Staji H, Darabighane B (2019) A comparison of the effects of resistant starch, fructooligosaccharide, and zinc bacitracin on cecal short-chain fatty acids, cecal microflora, intestinal morphology, and antibody titer against Newcastle disease virus in broilers. Comparative Clinical Pathology 28, 661–667.
| A comparison of the effects of resistant starch, fructooligosaccharide, and zinc bacitracin on cecal short-chain fatty acids, cecal microflora, intestinal morphology, and antibody titer against Newcastle disease virus in broilers.Crossref | GoogleScholarGoogle Scholar |
Akhavan-Salamat H, Ghasemib HA (2019) Effect of different sources and contents of zinc on growth performance, carcass characteristics, humoral immunity and antioxidant status of broiler chickens exposed to high environmental temperatures. Livestock Science 223, 76–83.
| Effect of different sources and contents of zinc on growth performance, carcass characteristics, humoral immunity and antioxidant status of broiler chickens exposed to high environmental temperatures.Crossref | GoogleScholarGoogle Scholar |
Aviagen Inc. (2009) ‘Arbor acres broiler management guide.’ (Newbridge: Huntsville, AL, USA)
Bartlett JR, Smith MO (2003) Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Poultry Science 82, 1580–1588.
| Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress.Crossref | GoogleScholarGoogle Scholar | 14601736PubMed |
Bonaventura P, Benedetti G, Albarède F, Miossec P (2015) Zinc and its role in immunity and inflammation. Autoimmunity Reviews 14, 277–285.
| Zinc and its role in immunity and inflammation.Crossref | GoogleScholarGoogle Scholar | 25462582PubMed |
Bun SD, Guo YM, Guo FC, Ji FJ, Cao H (2011) Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poultry Science 90, 1220–1226.
| Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella.Crossref | GoogleScholarGoogle Scholar | 21597062PubMed |
Calder PC (2014) Biomarkers of immunity and inflammation for use in nutrition interventions: International Life Sciences Institute European Branch work on selection criteria and interpretation. Endocrine 14, 236–244.
Ezzati MS, Bozorgmehrifard MH, Bijanzad P, Rasoulinezhad S, Moomivand H, Faramarzi S, Ghaedi A, Ghabel H, Stabraghi E (2013) Effects of different levels of zinc supplementation on broilers performance and immunity response to Newcastle disease vaccine. European Journal of Experimental Biology 34, 497–501.
Feng J, Ma WH, Wu XM, Wang Y, Feng J (2010) Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biological Trace Element Research 133, 203–211.
| Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers.Crossref | GoogleScholarGoogle Scholar | 19551351PubMed |
Hafez A, Nassef E, Fahmy M, Elsabagh M, Bakr A, Hegazi E (2019) Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens. Environmental Science and Pollution Research.
| Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 31104235PubMed |
Hambidge M (2003) Biomarkers of trace mineral intake and status. The Journal of Nutrition 133, 948S–955S.
| Biomarkers of trace mineral intake and status.Crossref | GoogleScholarGoogle Scholar | 12612181PubMed |
Huang YL, Lu L, Luo XG, Liu B (2007) An optimal dietary zinc level of broiler chicks fed a corn–soybean meal diet. Poultry Science 86, 2582–2589.
| An optimal dietary zinc level of broiler chicks fed a corn–soybean meal diet.Crossref | GoogleScholarGoogle Scholar | 18029804PubMed |
Huang YL, Lu L, Li SF, Luo XG, Liu B (2009) Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet. Journal of Animal Science 87, 2038–2046.
| Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet.Crossref | GoogleScholarGoogle Scholar | 19213702PubMed |
Iovino L, Cooper K, Kinsella S, DeRoos P, Jain R, Galimberti S, Orciuolo E, Benedetti E, Petrini M, Dudakov JA (2018) Zinc supplementation improves T cell reconstitution after allogeneic HSCT by stimulating endogenous pathways of thymic regeneration. Blood 132, 3321
| Zinc supplementation improves T cell reconstitution after allogeneic HSCT by stimulating endogenous pathways of thymic regeneration.Crossref | GoogleScholarGoogle Scholar |
Kloubert V, Blaabjerg K, Dalgaard TS, Poulsen HD, Rink L, Wessels I (2018) Influence of zinc supplementation on immune parameters in weaned pigs. Journal of Trace Elements in Medicine and Biology 49, 231–240.
| Influence of zinc supplementation on immune parameters in weaned pigs.Crossref | GoogleScholarGoogle Scholar | 29402502PubMed |
Kucuk O, Sahin N, Sahin K (2003) Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens. Biological Trace Element Research 94, 225–235.
| Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 12972690PubMed |
Kumari D, Nair N, Bedwal RS (2019) Morphological changes in spleen after dietary zinc deficiency and supplementation in Wistar rats. Pharmacological Reports 71, 206–217.
| Morphological changes in spleen after dietary zinc deficiency and supplementation in Wistar rats.Crossref | GoogleScholarGoogle Scholar | 30785058PubMed |
Kwiecień M, Winiarska-Mieczan A, Milczarek A, Tomaszewska E, Matras J (2016) Effects of zinc glycine chelate on growth performance, carcass characteristics, bone quality, and mineral content in bone of broiler chicken. Livestock Science 191, 43–50.
| Effects of zinc glycine chelate on growth performance, carcass characteristics, bone quality, and mineral content in bone of broiler chicken.Crossref | GoogleScholarGoogle Scholar |
Lai PW, Liang J, Hsia LC, Loh TC, Ho YW (2010) Effects of varying dietary zinc levels and environmental temperatures on the growth performance, feathering score and feather mineral concentrations of broiler chicks. Asian-Australasian Journal of Animal Sciences 23, 937–945.
| Effects of varying dietary zinc levels and environmental temperatures on the growth performance, feathering score and feather mineral concentrations of broiler chicks.Crossref | GoogleScholarGoogle Scholar |
Leeson S, Taylorpickard JA, Tucker LA (2005) Trace mineral requirements of poultry: validity of the NRC recommendations. Re-defining Mineral Nutrition 107–117.
Li F, Cong T, Li Z, Zhao L (2015) Effects of zinc deficiency on the relevant immune function in rats with sepsis induced by endotoxin/lipopolysaccharide. Europe PMC 31, 361–366.
Li M, Huang J, Tsai Y, Mao S, Fu C, Lien T (2016) Nanosize of zinc oxide and the effects on zinc digestibility, growth performances, immune response and serum parameters of weanling piglets. Animal Science Journal 87, 1379–1385.
| Nanosize of zinc oxide and the effects on zinc digestibility, growth performances, immune response and serum parameters of weanling piglets.Crossref | GoogleScholarGoogle Scholar | 26805551PubMed |
Liao XD, Li A, Lu L, Liu SB, Li SF, Zhang LY, Wang GY, Luo XG (2013) Optimal dietary zinc levels of broiler chicks fed a corn–soybean meal diet from 22 to 42 days of age. Animal Production Science 53, 388–394.
| Optimal dietary zinc levels of broiler chicks fed a corn–soybean meal diet from 22 to 42 days of age.Crossref | GoogleScholarGoogle Scholar |
Luecke RW, Simonel CE, Fraker PJ (1978) The effect of restricted dietary intake on the antibody mediated response of the zinc deficient A/J mouse. The Journal of Nutrition 108, 881–887.
| The effect of restricted dietary intake on the antibody mediated response of the zinc deficient A/J mouse.Crossref | GoogleScholarGoogle Scholar | 347040PubMed |
Maares M, Haase H (2016) Zinc and immunity: an essential interrelation. Archives of Biochemistry and Biophysics 611, 58–65.
| Zinc and immunity: an essential interrelation.Crossref | GoogleScholarGoogle Scholar | 27021581PubMed |
Maywald M, Wang F, Rink L (2018) Zinc supplementation plays a crucial role in T helper 9 differentiation in allogeneic immune reactions and non-activated T cells. Journal of Trace Elements in Medicine and Biology 50, 482–488.
| Zinc supplementation plays a crucial role in T helper 9 differentiation in allogeneic immune reactions and non-activated T cells.Crossref | GoogleScholarGoogle Scholar | 29439842PubMed |
Moghaddam HN, Jahanian R (2009) Immunological responses of broiler chicks can be modulated by dietary supplementation of zinc-methionine in place of inorganic zinc sources. Asian–Australasian Journal of Animal Sciences 22, 396–403.
| Immunological responses of broiler chicks can be modulated by dietary supplementation of zinc-methionine in place of inorganic zinc sources.Crossref | GoogleScholarGoogle Scholar |
Mwangi S, Timmons J, Ao T, Paul M, Macalintal L, Pescatore A, Cantor A, Ford M, Dawson KA (2017) Effect of zinc imprinting and replacing inorganic zinc with organic zinc on early performance of broiler chicks. Poultry Science 96, 861–868.
National Research Council (1994) ‘Nutrient requirements of poultry.’ 9th edn. (National Academies Press: Washington, DC, USA)
NY/T 33 (2004) ‘Feeding standard of chicken.’ Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Beijing.
Park SY, Birkhold SG, Kubena LF, Nisbet DJ, Ricke SC (2004) Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction. Biological Trace Element Research 101, 147–163.
| Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction.Crossref | GoogleScholarGoogle Scholar | 15557678PubMed |
Pimentel JL, Cook ME, Greger JL (1991a) Immune response of chicks fed various levels of zinc. Poultry Science 70, 947–954.
| Immune response of chicks fed various levels of zinc.Crossref | GoogleScholarGoogle Scholar | 1876570PubMed |
Pimentel JL, Cook ME, Greger JL (1991b) Research note: bioavailability of zinc-methionine for chicks. Poultry Science 70, 1637–1639.
| Research note: bioavailability of zinc-methionine for chicks.Crossref | GoogleScholarGoogle Scholar |
Prasad AS (2014) Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging. Journal of Trace Elements in Medicine and Biology 28, 364–371.
| Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging.Crossref | GoogleScholarGoogle Scholar | 25200490PubMed |
Qin SZ, Lin L, Zhang X, Liao XD, Zhang LY, Guo YL, Luo XG (2017) An optimal dietary zinc level of brown-egg laying hens fed a corn–soybean meal diet. Biological Trace Element Research 177, 376–383.
| An optimal dietary zinc level of brown-egg laying hens fed a corn–soybean meal diet.Crossref | GoogleScholarGoogle Scholar |
Raiten DJ, Sakr Ashour FA, Ross AC, Meydani SN, Dawson HD, Stephensen CB, Brabin BJ, Suchdev PS, Van OB, Group IC (2015) Inflammation and nutritional science for programs/policies and interpretation of research evidence (INSPIRE). The Journal of Nutrition 145, 1039S–1108S.
| Inflammation and nutritional science for programs/policies and interpretation of research evidence (INSPIRE).Crossref | GoogleScholarGoogle Scholar | 25833893PubMed |
Ray CS, Singh B, Jena I, Behera S, Ray S (2017) Low alkaline phosphatase (ALP) in adult population an indicator of zinc (Zn) and magnesium (Mg) deficiency. Current Research in Nutrition and Food Science 5, 347–352.
| Low alkaline phosphatase (ALP) in adult population an indicator of zinc (Zn) and magnesium (Mg) deficiency.Crossref | GoogleScholarGoogle Scholar |
Read SA, Obeid S, Ahlenstiel C, Ahlenstiel G (2019) The role of zinc in antiviral immunity. Advances in Nutrition 10, 696–710.
| The role of zinc in antiviral immunity.Crossref | GoogleScholarGoogle Scholar | 31305906PubMed |
Rink L (2000) Zinc and the immune system. The Proceedings of the Nutrition Society 59, 541–552.
| Zinc and the immune system.Crossref | GoogleScholarGoogle Scholar | 11115789PubMed |
Rosenkranz E, Metz CHD, Maywald M, Hilgers R, Weßels I, Senff T, Haase H, Jäger M, Ott M, Aspinall R, Plümäkers B, Rink L (2016) Zinc supplementation induces regulatory T cells by inhibition of Sirt-1 deacetylase in mixed lymphocyte cultures. Molecular Nutrition and Food Research 60, 661–671.
| Zinc supplementation induces regulatory T cells by inhibition of Sirt-1 deacetylase in mixed lymphocyte cultures.Crossref | GoogleScholarGoogle Scholar | 26614004PubMed |
Rossi P, Rutz F, Anciuti MA, Rech JL, Zauk NHF (2007) Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. Journal of Applied Poultry Research 16, 219–225.
| Influence of graded levels of organic zinc on growth performance and carcass traits of broilers.Crossref | GoogleScholarGoogle Scholar |
Salim HM, Lee HR, Jo C, Lee SK, Lee BD (2012) Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. Biological Trace Element Research 147, 120–129.
| Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 22167309PubMed |
Seo H, Cho Y, Kim T, Shin H, Kwun I (2010) Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3–E1 cells. Nutrition Research and Practice 4, 356–361.
| Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3–E1 cells.Crossref | GoogleScholarGoogle Scholar | 21103080PubMed |
Shinde P, Dass RS, Garg AK, Chaturvedi VK, Kumar R (2006) Effect of zinc supplementation from different sources on growth, nutrient digestibility, blood metabolic profile, and immune response of male Guinea pigs. Biological Trace Element Research 112, 247–262.
| Effect of zinc supplementation from different sources on growth, nutrient digestibility, blood metabolic profile, and immune response of male Guinea pigs.Crossref | GoogleScholarGoogle Scholar | 17057264PubMed |
Siva S, Rubin DT, Gulotta G, Wroblewski K, Pekow J (2017) Zinc deficiency is associated with poor clinical outcomes in patients with inflammatory bowel disease. Inflammatory Bowel Diseases 23, 152–157.
| Zinc deficiency is associated with poor clinical outcomes in patients with inflammatory bowel disease.Crossref | GoogleScholarGoogle Scholar | 27930412PubMed |
Sloup V, Jankovská I, Nechybová S, Peřinková P, Langrová I (2017) Zinc in the animal organism: a review. Scientia Agriculturae Bohemica 48, 13–21.
| Zinc in the animal organism: a review.Crossref | GoogleScholarGoogle Scholar |
Song Z, Jiang W, Liu Y, Wu P, Jiang J, Zhou X, Kuang S, Tang L, Tang W, Zhang Y, Feng L (2017) Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-κB, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella). Fish & Shellfish Immunology 66, 497–523.
| Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF-κB, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella).Crossref | GoogleScholarGoogle Scholar |
Squibb RL, Beisel WR, Bostian KA (1971) Effect of Newcastle disease on serum copper, zinc, cholesterol, and carotenoid values in the chick. Applied Microbiology 22, 1096–1099.
Sridhar K, Nagalakshmi D, Ramarao SV (2015) Effect of graded concentration of organic zinc (zinc glycinate) on skin quality, hematological and serum biochemical constituents in broiler chicken. The Indian Journal of Animal Sciences 85, 643–648.
Stahl JL, Greger JL, Cook ME (1989) Zinc, copper and iron utilisation by chicks fed various concentrations of zinc. British Poultry Science 30, 123–134.
| Zinc, copper and iron utilisation by chicks fed various concentrations of zinc.Crossref | GoogleScholarGoogle Scholar | 2743167PubMed |
Suzuki T, Katsumata S, Matsuzaki H, Suzuki K (2016) A short-term zinc-deficient diet decreases bone formation through down-regulated BMP2 in rat bone. Bioscience, Biotechnology, and Biochemistry 80, 1433–1435.
| A short-term zinc-deficient diet decreases bone formation through down-regulated BMP2 in rat bone.Crossref | GoogleScholarGoogle Scholar | 26931551PubMed |
Swain PS, Rao SBN, Rajendran D, Dominic G, Selvaraju S (2016) Nano zinc, an alternative to conventional zinc as animal feed supplement: a review. Animal Nutrition 2, 134–141.
| Nano zinc, an alternative to conventional zinc as animal feed supplement: a review.Crossref | GoogleScholarGoogle Scholar | 29767083PubMed |
Tronina W, Kinal S, Lubojemska B (2007) Effect of various forms of zinc applied in concentrate mixtures for broiler chickens on its bioavailability as well as meat composition and quality. Polish Journal of Food and Nutrition Sciences 57, 577–581.
Tsai YH, Mao SY, Li MZ, Huang JT, Lien TF (2016) Effects of nanosize zinc oxide on zinc retention, eggshell quality, immune response and serum parameters of aged laying hens. Animal Feed Science and Technology 213, 99–107.
| Effects of nanosize zinc oxide on zinc retention, eggshell quality, immune response and serum parameters of aged laying hens.Crossref | GoogleScholarGoogle Scholar |
Vieira MM, Ribeiro AML, Kessler AM, Moraes ML, Kunrath MA, Ledur VS (2013) Different sources of dietary zinc for broilers submitted to immunological, nutritional, and environmental challenge. Journal of Applied Poultry Research 22, 855–861.
| Different sources of dietary zinc for broilers submitted to immunological, nutritional, and environmental challenge.Crossref | GoogleScholarGoogle Scholar |
Wong CP, Rinaldi NA, Ho E (2015) Zinc deficiency enhanced inflammatory response by increasing immune cell activation and inducing IL6 promoter demethylation. Molecular Nutrition & Food Research 59, 991–999.
| Zinc deficiency enhanced inflammatory response by increasing immune cell activation and inducing IL6 promoter demethylation.Crossref | GoogleScholarGoogle Scholar |
Yalçinkaya I, Çinar M, Yildirim E, Erat S, Başalan M, Güngör T (2012) The effect of prebiotic and organic zinc alone and in combination in broiler diets on the performance and some blood parameters. Italian Journal of Animal Science 11, 298–302.
| The effect of prebiotic and organic zinc alone and in combination in broiler diets on the performance and some blood parameters.Crossref | GoogleScholarGoogle Scholar |
Zakaria HA, Jalal M, AL-Titi HH, Souad A (2017) Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers. Brazilian Journal of Poultry Science 19, 519–526.
| Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers.Crossref | GoogleScholarGoogle Scholar |
Zastrow ML, Pecoraro VL (2014) Designing hydrolytic zinc metalloenzymes. Biochemistry 53, 957–978.
| Designing hydrolytic zinc metalloenzymes.Crossref | GoogleScholarGoogle Scholar | 24506795PubMed |
Zhang Q, Zhang HX, Chen Y, Wang Y, Yang M, Guo M (2020) Zinc deficiency induces oxidative damage and causes spleen fibrosis. Biological Trace Element Research 194, 203–209.
| Zinc deficiency induces oxidative damage and causes spleen fibrosis.Crossref | GoogleScholarGoogle Scholar | 31172426PubMed |
