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RESEARCH ARTICLE

Impact of a tannin extract on animal performance and nitrogen excretion of dairy cows grazing a tropical pasture

T. Orlandi A , C. A. Pozo A , J. Schiavo B , L. Oliveira B and G. V. Kozloski https://orcid.org/0000-0002-4561-3770 A C
+ Author Affiliations
- Author Affiliations

A Departamento de Zootecnia, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.

B Departamento de Ciências Agrárias, Universidade do Noroeste do Estado do Rio Grande do Sul, Ijuí, RS, 98700-000, Brazil.

C Corresponding author. Email: gilberto.kozloski@ufsm.br

Animal Production Science 60(9) 1183-1188 https://doi.org/10.1071/AN18726
Submitted: 23 November 2018  Accepted: 2 December 2019   Published: 3 March 2020

Abstract

Context: Tannin extracts have been investigated as natural feed additives with the potential to decrease the enteric emission of methane and urinary N and to improve the productive performance of ruminants. However, the impact of this additive in dairy cattle grazing tropical grass pastures has not been extensively evaluated.

Aims: To evaluate the impact of the Acacia mearnsii bark extract (TA) on productive and nutritional variables in dairy cows grazing a Cynodon dactylon pasture and receiving supplementary corn silage and concentrate.

Methods: Fourteen multiparous Holstein dairy cows were assigned in a randomised block design to either of the following two treatments: concentrate without TA (control) or with 10 g of TA/kg dry matter (DM). The concentrate and corn silage accounted for ~0.30 and 0.30 of total DM intake respectively.

Key results: The dietary concentration of TA was 2.9 g/kg DM and it did not affect nutrients intake, milk production and composition, rumen microbial protein synthesis, plasma urea concentration or faecal N excretion. Total N and urea N excreted in urine were higher, whereas total N excreted in manure tended to be higher in TA treatment.

Conclusions: No productive advantage was obtained by including TA in the diet of dairy cows grazing Cynodon dactylon pasture. Instead, it increased the excretion of labile urinary N.

Implications: The effect of the use of TA as a feed additive for cows fed tropical grass-based diets is negative from the environmental point of view.

Additional keywords: Acacia mearnsii, Cynodon dactylon, excretions, milk yield, nitrogen utilisation.


References

Alves TP, Dall-Orsoletta A, Ribeiro Filho HMN (2017a) The effects of supplementing Acacia mearnsii tannin extract on dairy cow dry matter intake, milk production, and methane emission in a tropical pasture. Tropical Animal Health and Production 49, 1663–1668.
The effects of supplementing Acacia mearnsii tannin extract on dairy cow dry matter intake, milk production, and methane emission in a tropical pasture.Crossref | GoogleScholarGoogle Scholar | 28801856PubMed |

Alves TP, Dias KM, Dallastra LJH, Fonseca BL, Ribeiro Filho HMN (2017b) Energy and tannin extract supplementation for dairy cows on annual winter pastures. Semina. Ciências Agrárias 38, 1017–1026.
Energy and tannin extract supplementation for dairy cows on annual winter pastures.Crossref | GoogleScholarGoogle Scholar |

AOAC (1997) ‘Official methods of analysis’. 16th edn. (Association of Official Analytical Chemists: Gaithersburg, MD)

Ávila SC, Kozloski GV, Orlandi T, Mezzomo MP, Stefanello S (2015) Impact of a tannin extract on digestibility, ruminal fermentation and duodenal flow of amino acids in steers fed maize silage and concentrate containing soybean meal or canola meal as protein source. The Journal of Agricultural Science 153, 943–953.
Impact of a tannin extract on digestibility, ruminal fermentation and duodenal flow of amino acids in steers fed maize silage and concentrate containing soybean meal or canola meal as protein source.Crossref | GoogleScholarGoogle Scholar |

Carulla JE, Kreuzer M, Machmüller A, Hess HD (2005) Supplementation of Acacia mearnsii tannins decreases methanogenesis and urinary nitrogen in forage-fed sheep. Australian Journal of Agricultural Research 56, 961–970.
Supplementation of Acacia mearnsii tannins decreases methanogenesis and urinary nitrogen in forage-fed sheep.Crossref | GoogleScholarGoogle Scholar |

Chen XB, Gomes MJ (1995) ‘Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives: an overview of the technical details.’ (International Feed Resources Unit, Rowett Research Institute: Aberdeen, UK)

Cook CW (1964) Symposium on nutrition of forage and pastures: collecting forage samples representative of ingested samples of grazing animals for nutritional studies. Journal of Animal Science 23, 265–270.
Symposium on nutrition of forage and pastures: collecting forage samples representative of ingested samples of grazing animals for nutritional studies.Crossref | GoogleScholarGoogle Scholar |

Czarnocki J, Sibbald IR, Evans EV (1961) The determination of chromic oxide in samples of feed and excreta by acid digestion and spectrophotometry. Canadian Journal of Animal Science 41, 167–179.
The determination of chromic oxide in samples of feed and excreta by acid digestion and spectrophotometry.Crossref | GoogleScholarGoogle Scholar |

Dijkstra J, Oenema O, Van Groenigen JW, Spek JW, Van Vuuren AM, Bannink A (2013) Diet effects on urine composition of cattle and N2O emissions. Animal 7, 292–302.
Diet effects on urine composition of cattle and N2O emissions.Crossref | GoogleScholarGoogle Scholar | 23739471PubMed |

Euclides VPB, Macedo MCM, Oliveira MP (1992) Avaliação de diferentes métodos para se estimar o valor nutritivo de forragens sob pastejo. Revista Brasileira de Zootecnia 21, 691–702.

Gerlach K, Pries M, Südekum KH (2018a) Effect of condensed tannin supplementation on in vivo nutrient digestibilities and energy values of concentrates in sheep. Small Ruminant Research 161, 57–62.
Effect of condensed tannin supplementation on in vivo nutrient digestibilities and energy values of concentrates in sheep.Crossref | GoogleScholarGoogle Scholar |

Gerlach K, Pries M, Tholen E, Schmithausen AJ, Büscher W, Südekum KH (2018b) Effect of condensed tannins in rations of lactating dairy cows on production variables and nitrogen use efficiency. Animal 12, 1847–1855.
Effect of condensed tannins in rations of lactating dairy cows on production variables and nitrogen use efficiency.Crossref | GoogleScholarGoogle Scholar | 29306350PubMed |

Grainger C, Clarke T, Auldist MJ, Beauchemin KA, McGinn SM, Waghorn GC, Eckard RJ (2009) Potential use of Acacia mearnsii condensed tannins to reduce methane emissions and nitrogen excretion from grazing dairy cows. Canadian Journal of Animal Science 89, 241–251.
Potential use of Acacia mearnsii condensed tannins to reduce methane emissions and nitrogen excretion from grazing dairy cows.Crossref | GoogleScholarGoogle Scholar |

Griffiths WM, Clark CEF, Clark DA, Waghorn GC (2013) Supplementing lactating dairy cows fed high-quality pasture with black wattle (Acacia mearnsii) tannin. Animal 7, 1789–1795.
Supplementing lactating dairy cows fed high-quality pasture with black wattle (Acacia mearnsii) tannin.Crossref | GoogleScholarGoogle Scholar | 23899456PubMed |

Hristov AN, Bannink A, Crompton LA, Huhtanen P, Kreuzer M, McGee M, Nozière P, Reynolds CK, Bayat AR, Yáñez-Ruiz DR, Dijkstra J, Kebreab E, Schwarm A, Shingfield KJ, Yu Z (2019) Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques. Journal of Dairy Science 102, 5811–5852.
Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques.Crossref | GoogleScholarGoogle Scholar | 31030912PubMed |

Koenig KM, Beauchemin KA (2018) Effect of feeding condensed tannins in high protein finishing diets containing corn distillers grains on ruminal fermentation, nutrient digestibility, and route of nitrogen excretion in beef cattle. Journal of Animal Science 96, 4398–4413.

Kozloski GV, Fiorentini G, Härter CJ, Bonnecarrère Sanchez LM (2005) Uso de creatinina como indicador de excreção urinária em ovinos. Ciência Rural 35, 98–102.
Uso de creatinina como indicador de excreção urinária em ovinos.Crossref | GoogleScholarGoogle Scholar |

Kozloski GV, Härter CJ, Hentz F, Ávila SC, Orlandi T, Stefanello CM (2012) Intake, digestibility and nutrients supply to wethers fed ryegrass and intraruminally infused with levels of Acacia mearnsii tannin extract. Small Ruminant Research 106, 125–130.
Intake, digestibility and nutrients supply to wethers fed ryegrass and intraruminally infused with levels of Acacia mearnsii tannin extract.Crossref | GoogleScholarGoogle Scholar |

Kraft G, Ortigues-Marty I, Durand D, Rémond D, Jarde T, Bequette B, Savary-Auzeloux I (2011) Adaptations of hepatic amino acid uptake and net utilisation contributes to nitrogen economy or waste in lambs fed nitrogen- or energy-deficient diets. Animal 5, 678–690.
Adaptations of hepatic amino acid uptake and net utilisation contributes to nitrogen economy or waste in lambs fed nitrogen- or energy-deficient diets.Crossref | GoogleScholarGoogle Scholar | 22439991PubMed |

Licitra G, Hernandez TM, Van Soest PJ (1996) Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology 57, 347–358.
Standardization of procedures for nitrogen fractionation of ruminant feeds.Crossref | GoogleScholarGoogle Scholar |

Makkar HPS (2003) Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research 49, 241–256.
Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds.Crossref | GoogleScholarGoogle Scholar |

Mertens DR (2002) Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing beakers or crucibles: collaborative study. Journal of the Association of Official Analytical Chemists 85, 1217–1240.

NRC (2001) ‘Nutrient requirements of dairy cattle.’ 7th edn. (National Academy Press: Washington, DC)

Orlandi T, Kozloski GV, Alves TP, Mesquita FR, Ávila SC (2015) Digestibility, ruminal fermentation and duodenal flux of amino acids in steers fed grass forage plus concentrate containing increasing levels of Acacia mearnsii tannin extract. Animal Feed Science and Technology 210, 37–45.
Digestibility, ruminal fermentation and duodenal flux of amino acids in steers fed grass forage plus concentrate containing increasing levels of Acacia mearnsii tannin extract.Crossref | GoogleScholarGoogle Scholar |

Patra AK, Saxena J (2011) Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. Journal of the Science of Food and Agriculture 91, 24–37.
Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition.Crossref | GoogleScholarGoogle Scholar | 20815041PubMed |

Ribeiro Filho HMN, Zimermann FC, Kozloski GV (2008) Baixa dosagem de óxido de cromo para estimativa da produção fecal em bovinos. Ciência Rural 38, 2567–2573.
Baixa dosagem de óxido de cromo para estimativa da produção fecal em bovinos.Crossref | GoogleScholarGoogle Scholar |

Senger CCD, Kozloski GV, Sanchez LMB, Mesquita FR, Alves TP, Castagnino DS (2008) Evaluation of autoclave procedures for fibre analysis in forage and concentrate feedstuffs. Animal Feed Science and Technology 146, 169–174.
Evaluation of autoclave procedures for fibre analysis in forage and concentrate feedstuffs.Crossref | GoogleScholarGoogle Scholar |

Spanghero M, Kowalski ZM (1997) Critical analysis of N balance experiments with lactating cows. Livestock Production Science 52, 113–122.
Critical analysis of N balance experiments with lactating cows.Crossref | GoogleScholarGoogle Scholar |

’t Mannetje L (2000) Measuring biomass of grassland vegetation. In ‘Field and laboratory methods for grassland and animal production research’. (Eds L’t Mannetje, RM Jones) pp. 151–177. (CABI Publishing: Wallingford, UK)

Wray-Cahen D, Metcalf JA, Backwell FRC, Bequette BJ, Brown DS, Sutton JD, Lobley GE (1997) Hepatic response to increased exogenous supply of plasma amino acids by infusion into the mesenteric vein of Holstein-Friesan cows in late gestation. British Journal of Nutrition 78, 913–930.
Hepatic response to increased exogenous supply of plasma amino acids by infusion into the mesenteric vein of Holstein-Friesan cows in late gestation.Crossref | GoogleScholarGoogle Scholar | 9497443PubMed |

Zanferari F (2017) Quitosana associada a grão de soja integral na alimentação de vacas leiteiras: I. Digestão, metabolismo e desempenho produtivo; II. Avaliação de metodologias para estimativas da digestibilidade aparente total e produção microbiana ruminal. PhD Thesis, Universidade de São Paulo, Brazil.