The mRNA and lncRNA landscape of the non-pregnant endometrium during the oestrus cycle in dairy goat
Xiaorui Liu A * , Lei Zhang A B * , Jiuzeng Cui A * , Sicheng Che A , Yuexia Liu A , Yue Zhang A , Binyun Cao A and Yuxuan Song A B
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
B Corresponding author. Email: zhanglei07dongke@163.com; yuxuan_song2016@163.com
Animal Production Science 59(10) 1803-1813 https://doi.org/10.1071/AN18426
Submitted: 19 March 2018 Accepted: 23 January 2019 Published: 8 March 2019
Abstract
Cyclic changes in the endometrium are essential for embryo implantation in mammals; many studies report that such changes constitute a complex process involving numerous molecular mediators. In the present study, goat endometria at oestrus Day 5 and oestrus Day 15 were selected to systematically analyse the transcriptome using strand-specific Ribo-Zero RNA sequencing. Over 120 million high-quality paired-end reads were generated and 440 400 transcripts were identified in the endometrial tissue of dairy goats. In total, 489 differentially expressed mRNAs and 854 differentially expressed long non-coding RNAs were identified when comparing the endometrium at goat endometria at oestrus Day 5 and oestrus Day 15. Neurotensin was found to play a potentially important role in the non-pregnant goat endometrium during the oestrus cycle. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses of the cis-target genes of the differentially expressed long non-coding RNAs showed that GO : 0005198 (structural molecule activity) and ko04510 (focal adhesion) might be involved in cyclic endometrial changes. Taken together, the resulting transcriptomic profiles elucidate global trends in mRNA and lncRNA expression in non-pregnant endometria during the oestrus cycle in dairy goats.
Additional keywords: RNA-Seq, embryo implantation, GO, KEGG.
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