Effective incorporation of xylanase and phytase in lick blocks for grazing livestock
R. J. Ainscough A , J. M. McGree A , M. J. Callaghan B and R. E. Speight A CA Queensland University of Technology (QUT), 2 George Street, Brisbane, Qld 4000, Australia.
B Ridley AgriProducts Pty Ltd, PO Box 905, Toowong, Qld 4066, Australia.
C Corresponding author. Email: robert.speight@qut.edu.au
Animal Production Science 59(9) 1762-1768 https://doi.org/10.1071/AN18424
Submitted: 24 July 2018 Accepted: 24 October 2018 Published: 27 November 2018
Abstract
The addition of feed enzymes to livestock diets has contributed to significant increases in productivity over recent decades. The use of enzymes has been the most common in systems where enzyme delivery and diets can be easily managed, such as for poultry and pigs. Lick blocks supplement the forage diets of ruminants with nitrogen and minerals but not enzymes, due in part to concerns that block manufacturing temperatures would lead to unacceptable levels of enzyme degradation. The nutritional value of low quality pasture could be improved using enzyme supplemented lick blocks if enzymes remain active at the high lick block manufacturing temperatures. The aim of this study was to determine the extent of xylanase and phytase activity survival when exposed to the production of hot poured lick blocks. Lick block formulations and methods of manufacturing vary, so two enzyme containing molasses-based lick blocks were produced, one at 60°C and another at 100°C. The results showed that both the xylanase and phytase enzymes have high levels of survival at 60°C. In the 100°C lick block, the phytase displayed a half-life of ~10 min, whereas the xylanase retained 90% of the original activity after 30 min of exposure. The inherent thermostability of the enzymes were critical factors for enzyme survival and the enzymes were more stable in the lick blocks than in solution. The results indicate that it should be possible to add enzymes to lick blocks manufactured at elevated temperatures to enhance low quality pasture and thereby aid ruminant digestion and production.
Additional keywords: enzyme stability, feed supplement, formulation.
References
Bedford MR, Schulze H (1998) Exogenous enzymes for pigs and poultry. Nutrition Research Reviews 11, 91–114.| Exogenous enzymes for pigs and poultry.Crossref | GoogleScholarGoogle Scholar |
Brask-Pedersen DN, Glitsø LV, Skov LK, Lund P, Sehested J (2013) Effect of exogenous phytase on degradation of inositol phosphate in dairy cows. Journal of Dairy Science 96, 1691–1700.
| Effect of exogenous phytase on degradation of inositol phosphate in dairy cows.Crossref | GoogleScholarGoogle Scholar |
EFSA Panel (2012) Scientific opinion on the safety and efficacy of and endo-1,(3)4-beta-glucanase) as a feed additive for poultry and piglets: Roxazyme G2 for poultry and piglets. EFSA Journal 10, 2930
| Scientific opinion on the safety and efficacy of and endo-1,(3)4-beta-glucanase) as a feed additive for poultry and piglets: Roxazyme G2 for poultry and piglets.Crossref | GoogleScholarGoogle Scholar |
EFSA Panel (2016) Safety and efficacy of RONOZYME® HiPhos (6-phytase) as a feed additive for sows and fish. EFSA Journal 14, 4393
Francesch M, Geraert PA (2009) Enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybean-based diets. Poultry Science 88, 1915–1924.
| Enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybean-based diets.Crossref | GoogleScholarGoogle Scholar |
Gómez-Vázquez A, Mendoza GD, Aranda E, Pérez J, Hernández A, Pinos-Rodríguez JM (2011) Influence of fibrolytic enzymes on growth performance and digestion in steers grazing stargrass and supplemented with fermented sugarcane. Journal of Applied Animal Research 39, 77–79.
| Influence of fibrolytic enzymes on growth performance and digestion in steers grazing stargrass and supplemented with fermented sugarcane.Crossref | GoogleScholarGoogle Scholar |
Hangara GN, Teweldemedhin MY, Groenewald IB (2011) Major constraints for cattle productivity and managerial efficiency in communal areas of Omaheke Region, Namibia. International Journal of Agricultural Sustainability 9, 495–507.
| Major constraints for cattle productivity and managerial efficiency in communal areas of Omaheke Region, Namibia.Crossref | GoogleScholarGoogle Scholar |
Kebreab E, Hansen AV, Strathe AB (2012) Animal production for efficient phosphate utilization: from optimized feed to high efficiency livestock. Current Opinion in Biotechnology 23, 872–877.
| Animal production for efficient phosphate utilization: from optimized feed to high efficiency livestock.Crossref | GoogleScholarGoogle Scholar |
Kincaid RL, Garikipati DK, Nennich TD, Harrison JH (2005) Effect of grain source and exogenous phytase on phosphorus digestibility in dairy cows. Journal of Dairy Science 88, 2893–2902.
| Effect of grain source and exogenous phytase on phosphorus digestibility in dairy cows.Crossref | GoogleScholarGoogle Scholar |
Kirk O, Borchert TV, Fuglsang CC (2002) Industrial enzyme applications. Current Opinion in Biotechnology 13, 345–351.
| Industrial enzyme applications.Crossref | GoogleScholarGoogle Scholar |
Knowlton KF, Taylor MS, Hill SR, Cobb C, Wilson KF (2007) Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase. Journal of Dairy Science 90, 4356–4360.
| Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase.Crossref | GoogleScholarGoogle Scholar |
McLennan SR, Callaghan MJ, Swain AJ, Kidd JF (2012) Effect of monensin inclusion in supplements for cattle consuming low quality tropical forage. Animal Production Science 52, 624–629.
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426–428.
| Use of dinitrosalicylic acid reagent for determination of reducing sugar.Crossref | GoogleScholarGoogle Scholar |
Patel AK, Singhania RR, Pandey A (2016) Novel enzymatic processes applied to the food industry. Current Opinion in Food Science 7, 64–72.
| Novel enzymatic processes applied to the food industry.Crossref | GoogleScholarGoogle Scholar |
Poppi DP, Quigley SP, da Silva TACC, McLennan SR (2018) Challenges of beef cattle production from tropical pastures. Brazilian Journal of Animal Science 47, e20160419
R Core Team (2016) ‘R: a language and environment for statistical computing.’ (R Core Team: Vienna, Austria)
Rebello S, Jose L, Sindhu R, Aneesh EM (2017) Molecular advancements in the development of thermostable phytases. Applied Microbiology and Biotechnology 101, 2677–2689.
| Molecular advancements in the development of thermostable phytases.Crossref | GoogleScholarGoogle Scholar |
Sansoucy R, Aarts G, Leng R (1992) Molasses/urea blocks. In ‘FAO tropical feeds database’. (FAO: Rome) Available at http://www.fao.org/livestock/agap/frg/ECONF95/HTML/MUB.HTM [Verified 6 July 2018]
Shivange AV, Roccatano D, Schwaneberg U (2016) Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution. Applied Microbiology and Biotechnology 100, 227–242.
| Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution.Crossref | GoogleScholarGoogle Scholar |
Stöckmann C, Losen M, Dahlems U, Knocke C, Gellissen G, Büchs J (2003) Effect of oxygen supply on passaging, stabilising and screening of recombinant Hansenula polymorpha production strains in test tube cultures. FEMS Yeast Research 4, 195–205.
| Effect of oxygen supply on passaging, stabilising and screening of recombinant Hansenula polymorpha production strains in test tube cultures.Crossref | GoogleScholarGoogle Scholar |
Yi JQ, Piao XS, Li ZC, Zhang HY, Chen Y, Li QY, Liu DJ, Zhang Q, Ru YJ, Dong B (2013) The effects of enzyme complex on performance, intestinal health and nutrient digestibility of weaned pigs. Asian-Australasian Journal of Animal Sciences 26, 1181–1188.
| The effects of enzyme complex on performance, intestinal health and nutrient digestibility of weaned pigs.Crossref | GoogleScholarGoogle Scholar |
Zhao H (2010) Methods for stabilizing and activating enzymes in ionic liquids – a review. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire) 85, 891–907.
| Methods for stabilizing and activating enzymes in ionic liquids – a review.Crossref | GoogleScholarGoogle Scholar |
Zhu X, Deyoe CW, Behnke KC, Seib PA (1991) Poured feed blocks using distillery by-products as supplements for ruminants. Journal of the Science of Food and Agriculture 54, 535–547.
| Poured feed blocks using distillery by-products as supplements for ruminants.Crossref | GoogleScholarGoogle Scholar |