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RESEARCH ARTICLE

Starch sources and concentration in diet of dairy goats affected ruminal pH and fermentation, and inflammatory response

Yizhao Shen A B , Fangfang Zhao A , Lihuai Yu A , Wenzhu Yang B , Mengzhi Wang A and Hongrong Wang A C
+ Author Affiliations
- Author Affiliations

A Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

B Agriculture and Agri-Food Canada, Lethbridge Research Centre, 1st Avenue South 5403, PO Box 3000, Lethbridge, Alberta, T1J 4B1, Canada.

C Corresponding author. Email: hrwang@yzu.edu.cn

Animal Production Science 59(9) 1640-1647 https://doi.org/10.1071/AN17758
Submitted: 4 November 2017  Accepted: 5 November 2018   Published: 7 December 2018

Abstract

Corn and wheat grains are two starch sources with considerably different ruminal digestion rates, which may lead to differing lipopolysaccharide (LPS) release in both rumen and hindgut affecting animal production. The objectives of this study were to (1) investigate the effects of different ruminal and faecal LPS concentrations induced by starch source (corn vs wheat) and starch concentrations (low vs high) on DMI, ruminal pH, ruminal fermentation patterns, milk production, and inflammatory responses; and (2) evaluate the possible translocation site of LPS in dairy goats. Eight lactating dairy goats with ruminal cannulas were used in a replicated 4 × 4 Latin square design with 2 × 2 factorial arrangement of treatments. Each experimental period consisted of 24 days long including 21 days for adaption and 3 days for data and sample collection. The four treatment diets were: corn and wheat grain combined with low (LS) and high grain starch (HS). Goats were fed equal amounts of a total mixed ration twice daily at 0700 hours and 1900 hours. Replacing corn with wheat in goat diet led to longer (P < 0.02) duration of ruminal pH <5.6, higher ruminal LPS (P < 0.05), but lower faecal LPS concentration. However, no differences between two grains in ruminal pH (mean, minimum and maximum), volatile fatty acids (VFA) and lactic acid concentration were observed. Goats fed HS diets had lower (P < 0.01) ruminal pH and higher (P < 0.01) ruminal concentrations of VFA and lactic acid, as well as higher (P < 0.01) ruminal and faecal LPS concentrations. Starch source did not affect DMI, milk yield and milk components whereas feeding HS versus LS diet had higher milk yield, lactose yield and improved milk efficiency (P < 0.05). Feeding wheat- versus corn-based diet showed only greater (P < 0.05) concentration of toll-like receptor-4, whereas feeding the HS versus LS diet consistently increased blood concentrations of amyloid A, haptoglobin, LPS binding protein, and LPS (P < 0.05). Analysis of Pearson correlation coefficients illustrated that the ruminal LPS concentration is more important than faecal LPS in inflammatory responses. In conclusion, replacing corn with wheat in lactating goat diet had negative impact on ruminal pH but little effects on fermentation characteristics and milk production. Increasing the dietary concentration of starch decreased ruminal pH status and thus increased risk of acidosis, whereas, feeding HS versus LS diets resulted in an improvement in milk yield, milk efficiency, and immunity response. Moreover, rumen acidosis induced by wheat based diet was accompanied with more severe inflammatory responses.

Additional keywords: acute-phase protein, corn, lipopolysaccharide, translocation, wheat.


References

AOAC (2005) ‘Official methods of analysis.’ 18th edn. (AOAC International: Gaithersburg, MD)

Barker SB, Summerson WH (1941) The colorimetric determination of lactic acid in biological material. The Journal of Biological Chemistry 138, 535–554.

Blum JW, Dosogne H, Hoeben D, Vangroenweghe F, Hammon HM, Bruckmaier RM, Burvenich C (2000) Tumor necrosis factor-α and nitrite/nitrate responses during acute mastitis induced by Escherichia coli infection and endotoxin in dairy cows. Domestic Animal Endocrinology 19, 223–235.
Tumor necrosis factor-α and nitrite/nitrate responses during acute mastitis induced by Escherichia coli infection and endotoxin in dairy cows.Crossref | GoogleScholarGoogle Scholar |

Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H (2012) Acute phase proteins in ruminants. Journal of Proteomics 75, 4207–4231.
Acute phase proteins in ruminants.Crossref | GoogleScholarGoogle Scholar |

Eckersall PD, Bell R (2010) Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine. Veterinary Journal (London, England) 185, 23–27.
Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine.Crossref | GoogleScholarGoogle Scholar |

Gozho GN, Krause DO, Plaizier JC (2006) Rumen lipopolysaccharide and inflammation during grain adaptation and subacute ruminal acidosis in steers. Journal of Dairy Science 89, 4404–4413.
Rumen lipopolysaccharide and inflammation during grain adaptation and subacute ruminal acidosis in steers.Crossref | GoogleScholarGoogle Scholar |

Gressley TF, Hall MB, Armentano LE (2011) Ruminant nutrition symposium: productivity, digestion, and health responses to hindgut acidosis in ruminants. Journal of Animal Science 89, 1120–1130.
Ruminant nutrition symposium: productivity, digestion, and health responses to hindgut acidosis in ruminants.Crossref | GoogleScholarGoogle Scholar |

Gulmez BH, Turkmen II (2007) Effect of starch sources with different degradation rates on ruminal fermentation of lactating dairy cows. Revue de Medecine Veterinaire 158, 92–99.

Herrera-Saldana RE, Huber JT, Poore MH (1990) Dry matter, crude protein, and starch degradability of five cereal grains. Journal of Dairy Science 73, 2386–2393.
Dry matter, crude protein, and starch degradability of five cereal grains.Crossref | GoogleScholarGoogle Scholar |

Huo WJ, Zhu WY, Mao SY (2013) Effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide in the rumen fluid and the subsequent alterations in immune responses in goats. Asian-Australasian Journal of Animal Sciences 26, 1437–1445.
Effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide in the rumen fluid and the subsequent alterations in immune responses in goats.Crossref | GoogleScholarGoogle Scholar |

Khafipour E, Krause DO, Plaizier JC (2009) A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science 92, 1060–1070.
A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation.Crossref | GoogleScholarGoogle Scholar |

Kleen JL, Hooijer GA, Rehage J, Noordhuizen JPTM (2003) Subacute ruminal acidosis (SARA): a review. Journal of Veterinary Medicine Series A-Physiology Pathology Clinical Medicine 50, 406–414.
Subacute ruminal acidosis (SARA): a review.Crossref | GoogleScholarGoogle Scholar |

Larsen M, Lund P, Weisbjerg MR, Hvelplund T (2009) Digestion site of starch from cereals and legumes in lactating dairy cows. Animal Feed Science and Technology 153, 236–248.
Digestion site of starch from cereals and legumes in lactating dairy cows.Crossref | GoogleScholarGoogle Scholar |

Li S, Khafipour E, Krause DO, Kroeker A, Rodriguez-Lecompte JC, Gozho GN, Plaizier JC (2012) Effects of subacute ruminal acidosis challenges on fermentation and endotoxins in the rumen and hindgut of dairy cows. Journal of Dairy Science 95, 294–303.
Effects of subacute ruminal acidosis challenges on fermentation and endotoxins in the rumen and hindgut of dairy cows.Crossref | GoogleScholarGoogle Scholar |

Li F, Cao YC, Liu NN, Yang XJ, Yao JH, Yan DB (2014) Subacute ruminal acidosis challenge changed in situ degradability of feedstuffs in dairy goats. Journal of Dairy Science 97, 5101–5109.
Subacute ruminal acidosis challenge changed in situ degradability of feedstuffs in dairy goats.Crossref | GoogleScholarGoogle Scholar |

Li S, Yoon I, Scott M, Khafipour E, Plaizier JC (2016) Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindgut of dairy cows. Animal Feed Science and Technology 211, 50–60.
Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindgut of dairy cows.Crossref | GoogleScholarGoogle Scholar |

Liu JH, Xu TT, Liu YJ, Zhu WY, Mao SY (2013) A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 305, R232–R241.
A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats.Crossref | GoogleScholarGoogle Scholar |

Liu JH, Xu TT, Zhu WY, Mao SY (2014) High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats. British Journal of Nutrition 112, 416–427.
High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.Crossref | GoogleScholarGoogle Scholar |

Mani V, Weber TE, Baumgard LH, Gabler NK (2012) Growth and development symposium: endotoxin, inflammation, and intestinal function in livestock. Journal of Animal Science 90, 1452–1465.
Growth and development symposium: endotoxin, inflammation, and intestinal function in livestock.Crossref | GoogleScholarGoogle Scholar |

McAllister TA, Sultana H (2011) Effects of micronization on the in situ and in vitro digestion of cereal grains. Asian-Australasian Journal of Animal Sciences 24, 929–939.
Effects of micronization on the in situ and in vitro digestion of cereal grains.Crossref | GoogleScholarGoogle Scholar |

Mertens DR (2002) Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.

Nocek JE, Tamminga S (1991) Site of digestion of starch in the gastrointestinal tract of dairy cows and its effect on milk yield and composition. Journal of Dairy Science 74, 3598–3629.
Site of digestion of starch in the gastrointestinal tract of dairy cows and its effect on milk yield and composition.Crossref | GoogleScholarGoogle Scholar |

NRC (2007) ‘Nutrient requirements of small ruminants: sheep, goats, cervids, and new worlds camelids.’ (The National Academies Press: Washington, DC)

Offner A, Bach A, Sauvant D (2003) Quantitative review of in situ starch degradation in the rumen. Animal Feed Science and Technology 106, 81–93.
Quantitative review of in situ starch degradation in the rumen.Crossref | GoogleScholarGoogle Scholar |

Penner GB, Beauchemin KA, Mutsvangwa T (2006) An evaluation of the accuracy and precision of a stand-alone submersible continuous ruminal pH measurement system. Journal of Dairy Science 89, 2132–2140.
An evaluation of the accuracy and precision of a stand-alone submersible continuous ruminal pH measurement system.Crossref | GoogleScholarGoogle Scholar |

Pilachai R, Schonewille JT, Thamrongyoswittayakul C, Aiumlamai S, Wachirapakorn C, Everts H, Hendriks WH (2012) Starch source in high concentrate rations does not affect rumen pH, histamine and lipopolysaccharide concentrations in dairy cows. Livestock Science 150, 135–142.
Starch source in high concentrate rations does not affect rumen pH, histamine and lipopolysaccharide concentrations in dairy cows.Crossref | GoogleScholarGoogle Scholar |

Plaizier JC, Krause DO, Gozho GN, McBride BW (2008) Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. Veterinary Journal (London, England) 176, 21–31.
Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences.Crossref | GoogleScholarGoogle Scholar |

Plaizier JC, Khafipour E, Li S, Gozho GN, Krause DO (2012) Subacute ruminal acidosis (SARA), endotoxins and health consequences. Animal Feed Science and Technology 172, 9–21.
Subacute ruminal acidosis (SARA), endotoxins and health consequences.Crossref | GoogleScholarGoogle Scholar |

Plaizier JC, Li S, Le Sciellour M, Schurmann BL, Górka P, Penner GB (2014) Effects of duration of moderate increases in grain feeding on endotoxins in the digestive tract and acute phase proteins in peripheral blood of yearling calves. Journal of Dairy Science 97, 7076–7084.
Effects of duration of moderate increases in grain feeding on endotoxins in the digestive tract and acute phase proteins in peripheral blood of yearling calves.Crossref | GoogleScholarGoogle Scholar |

Sharif A, Muhammad G (2008) Somatic cell count as an indicator of udder health status under modern dairy production: A review. Pakistan Veterinary Journal 28, 194–200.

Stone W (2004) Nutritional approaches to minimize subacute ruminal acidosis and laminitis in dairy cattle. Journal of Dairy Science 87, E13–E26.
Nutritional approaches to minimize subacute ruminal acidosis and laminitis in dairy cattle.Crossref | GoogleScholarGoogle Scholar |

Suffredini AF, Fantuzzi G, Badolato R, Oppenheim JJ, O’Grady NP (1999) New insights into the biology of the acute phase response. Journal of Clinical Immunology 19, 203–214.
New insights into the biology of the acute phase response.Crossref | GoogleScholarGoogle Scholar |

Sweet MJ, Hume DA (1996) Endotoxin signal transduction in macrophages. Journal of Leukocyte Biology 60, 8–26.
Endotoxin signal transduction in macrophages.Crossref | GoogleScholarGoogle Scholar |

Tothova C, Nagy O, Kovac G (2014) Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Veterinarni Medicina 59, 163–180.
Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review.Crossref | GoogleScholarGoogle Scholar |

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar |

Zebeli Q, Ametaj BN (2009) Relationships between rumen lipopolysaccharide and mediators of inflammatory response with milk fat production and efficiency in dairy cows. Journal of Dairy Science 92, 3800–3809.
Relationships between rumen lipopolysaccharide and mediators of inflammatory response with milk fat production and efficiency in dairy cows.Crossref | GoogleScholarGoogle Scholar |