Effects of pre-slaughter ascorbic acid administration on some physiological stress response and meat quality traits of lambs and kids subjected to road transport
Muammer Sarıbey A and Serhat Karaca A BA Department of Animal Science, Faculty of Agriculture, Van Yuzuncu Yıl University, 65080-Van, Turkey.
B Corresponding author. Email: serhatkaraca@gmail.com
Animal Production Science 59(5) 954-964 https://doi.org/10.1071/AN17554
Submitted: 9 August 2017 Accepted: 24 April 2018 Published: 19 June 2018
Abstract
The present study determined the effects of pre-slaughter ascorbic acid administration on some physiological indicators and meat quality traits of road transport-stressed lambs and kids. Eighteen lambs and 18 kids were divided into the following three groups: no transportation (T0), 3-h transportation (T3), and ascorbid acid administered at 125 mg/kg before 3-h transportation (T3+C). The liveweight loss and rectal temperature were similar, while there were significant differences among groups for some biochemical parameters in both lambs and kids. Glucose concentrations significantly increased after transportation in lambs (P = 0.005) and kids (P < 0.001). Ascorbic acid had significant effects on serum glucose of lambs. T3 lambs had higher glucose concentrations than did T0 lambs (P < 0.001), while T0 and T3+C lambs were found to have similar concentrations. Oxidative status in lambs and kids, as determined by serum malondialdehyde concentration, did not differ among groups. However, some meat quality traits were negatively influenced by transportation. T3 lambs had a significantly lower muscle glycogen concentration than did T0 and T3+C lambs (P = 0.018), while ultimate pH was similar among groups. Muscle glycogen concentration was significantly lower (P = 0.008) and ultimate pH was higher in T3 and T3+C kids (P = 0.028). T3 and T3+C groups had darker meat (L*) than did T0 groups both in lambs and kids, whereas a*, cooking loss and water-holding capacity were similar among groups. The effect of ascorbic acid on meat toughness was significant and shear force value in T0 lambs was lower than that in T3 lambs (P = 0.009), while T0 and T3+C lambs were found to have similar shear force values. Overall, the administration of ascorbic acid to lambs before transportation could partly reduce the adverse effects of road transportation stress, whereas its effects on kids were found to be insignificant.
Additional keywords: preslaughter handling, ruminants, stress physiology.
References
Adzitey F (2011) Effect of pre-slaughter animal handling on carcass and meat quality. International Food Research Journal 18, 484–490.Alcalde MJ, Suárez MD, Rodero E, Álvarez R, Sáez MI, Martínez TF (2017) Effects of farm management practices and transport duration on stress response and meat quality traits of suckling goat kids. Animal 11, 1626–1635.
Anonymous (2018) ‘Meterological data system (MEVBIS).’ (Turkish State Meterological Service: Van, Turkey)
Apple JK, Dikeman ME, Minton JE, McMurphy RM, Fedde MR, Leith DE, Unruh JA (1995) Effects of restraint and isolation stress and epidural blockade on endocrine and blood metabolite status, muscle glycogen metabolism, and incidence of dark-cutting longissimus muscle of sheep. Journal of Animal Science 73, 2295–2307.
| Effects of restraint and isolation stress and epidural blockade on endocrine and blood metabolite status, muscle glycogen metabolism, and incidence of dark-cutting longissimus muscle of sheep.Crossref | GoogleScholarGoogle Scholar |
Avci G, Küçükkurt İ, Fİdan F, Eryavuz A, Aslan R, Dündar Y (2008) The influence of vitamin C and xylazine on cortisol, lipid peroxidation and some biochemical parameters in transported sheep. Fırat University Veterinary Journal of Health Sciences 22, 147–152.
Bánhegyi G, Mandl J (2001) The hepatic glycogenoreticular system. Pathology Oncology Research 7, 107–110.
| The hepatic glycogenoreticular system.Crossref | GoogleScholarGoogle Scholar |
Bonvillani A, Pena F, de Gea G, Gomez G, Petryna A, Perea J (2010) Carcass characteristics of Criollo Cordobes kid goats under an extensive management system: effects of gender and liveweight at slaughter. Meat Science 86, 651–659.
| Carcass characteristics of Criollo Cordobes kid goats under an extensive management system: effects of gender and liveweight at slaughter.Crossref | GoogleScholarGoogle Scholar |
Bórnez R, Linares MB, Vergara H (2009) Haematological, hormonal and biochemical blood parameters in lamb: effect of age and blood sampling time. Livestock Science 121, 200–206.
| Haematological, hormonal and biochemical blood parameters in lamb: effect of age and blood sampling time.Crossref | GoogleScholarGoogle Scholar |
Burtis CA, Ashwood ER, Bruns DE (2013) ‘Tietz textbook of clinical chemistry and molecular diagnostics.’ (Elseiver Saunders: Singapore: MO)
Carroll NV, Longley RW, Roe JH (1956) The determination of glycogen in liver and muscle by use of anthrone reagent. The Journal of Biological Chemistry 220, 583–593.
Çetin E, Çetin N, Küçük O (2011) The effect of road transport on some haematological and biochemical parameters in yearling lambs. Journal of Faculty of Veterinary Medicine. Erciyes University 8, 97–103.
Cockram MS, Kent JE, Goddard PJ, Waran NK, McGilp IM, Jackson RE, Muwanga GM, Prytherch S (1996) Effect of space allowance during transport on the behavioural and physiological responses of lambs during and after transport. Animal Science 62, 461–477.
| Effect of space allowance during transport on the behavioural and physiological responses of lambs during and after transport.Crossref | GoogleScholarGoogle Scholar |
Colomer-Rocher F, Morand-Fehr P, Kirton AH (1987) Standard methods and procedures for goat carcass evaluation, jointing and tissue separation. Livestock Production Science 17, 149–159.
| Standard methods and procedures for goat carcass evaluation, jointing and tissue separation.Crossref | GoogleScholarGoogle Scholar |
Council E 2005
Ekiz B, Ekiz EE, Yalçıntan H, Koçak Ö, Yılmaz A, Güneş H (2012) The effects of transport stress on certain welfare parameters and behaviours in Red Karaman, Imroz, Sakız and Karakul rams. Istanbul Üniversitesi Veteriner Fakültesi Dergisi 38, 15–28.
Fuquay JW (1981) Heat stress as it affects animal production. Journal of Animal Science 52, 164–174.
| Heat stress as it affects animal production.Crossref | GoogleScholarGoogle Scholar |
Grandin T (1998) Cattle. In ‘Animal welfare and meat science’. (Ed. T Grandin) pp. 123–145. (CABI: New York)
Hoffman LC, Muller M, Cloete SWP, Schmidt D (2003) Comparison of six crossbred lamb types: sensory, physical and nutritional meat quality characteristics. Meat Science 65, 1265–1274.
| Comparison of six crossbred lamb types: sensory, physical and nutritional meat quality characteristics.Crossref | GoogleScholarGoogle Scholar |
Idrus Z, Bahyuddin N, Wai CY, Farjam AS, Sazili AQ, Rajion MA, Meng GY (2010) Physiological responses in goats subjected to road transportation under the hot, humid tropical conditions. International Journal of Agriculture and Biology 12, 840–844.
Kadim IT, Mahgoub O, Al-Kindi A, Al-Marzooqi W, Al-Saqri NM (2006) Effects of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics of three breeds of Omani goats. Meat Science 73, 626–634.
| Effects of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics of three breeds of Omani goats.Crossref | GoogleScholarGoogle Scholar |
Kadim IT, Mahgoub O, AlKindi AY, Al-Marzooqi W, Al-Saqri NM, Almaney M, Mahmoud IY (2007) Effect of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics in two age groups of Omani sheep. Asian-Australasian Journal of Animal Sciences 20, 424–431.
| Effect of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics in two age groups of Omani sheep.Crossref | GoogleScholarGoogle Scholar |
Kadim IT, Mahgoub O, Al-Marzooqi W, Khalaf S, Al-Sinawi SSH, Al-Amri IS (2009) Effects of transportation during the hot season and low voltage electrical stimulation on histochemical and meat quality characteristics of sheep longissimus muscle. Livestock Science 126, 154–161.
| Effects of transportation during the hot season and low voltage electrical stimulation on histochemical and meat quality characteristics of sheep longissimus muscle.Crossref | GoogleScholarGoogle Scholar |
Kannan G, Terrill TH, Kouakou B, Gelaye S, Amoah EA (2002) Simulated preslaughter holding and isolation effects on stress responses and live weight shrinkage in meat goats1. Journal of Animal Science 80, 1771–1780.
| Simulated preslaughter holding and isolation effects on stress responses and live weight shrinkage in meat goats1.Crossref | GoogleScholarGoogle Scholar |
Kannan G, Kouakou B, Terrill TH, Gelaye S (2003) Endocrine, blood metabolite, and meat quality changes in goats as influenced by short-term, preslaughter stress. Journal of Animal Science 81, 1499–1507.
| Endocrine, blood metabolite, and meat quality changes in goats as influenced by short-term, preslaughter stress.Crossref | GoogleScholarGoogle Scholar |
Karaca S, Erdoğan S, Kor D, Kor A (2016) Effects of pre-slaughter diet/management system and fasting period on physiological indicators and meat quality traits of lambs. Meat Science 116, 67–77.
| Effects of pre-slaughter diet/management system and fasting period on physiological indicators and meat quality traits of lambs.Crossref | GoogleScholarGoogle Scholar |
Karanth S, Yu WH, Walczewska A, Mastronardi C, McCann SM (2000) Ascorbic acid acts as an inhibitory transmitter in the hypothalamus to inhibit stimulated luteinizing hormone-releasing hormone release by scavenging nitric oxide. Proceedings of the National Academy of Sciences, USA 97, 1891–1896.
| Ascorbic acid acts as an inhibitory transmitter in the hypothalamus to inhibit stimulated luteinizing hormone-releasing hormone release by scavenging nitric oxide.Crossref | GoogleScholarGoogle Scholar |
Kassab AY, Mohammed AA (2014) Ascorbıc acid administration as anti-stress before transportatıon of sheep. Egyptian Journal of Animal Production 51, 19–25.
Khalid T, Biobaku JM, Ismail AO, Onifade KI, Ismaila MS, Agaie BM (2016) Ascorbic acid supplementation improves the quality of meat characteristics in Sahel bucks exposed to long distance road transport. Alexandria Journal of Veterinary Sciences 5, 24–30.
Maria GA (2008) Meat quality. In ‘Long distance transport and welfare of farm animals’. (Eds MC Appleby, V Cussen, L Garces) pp. 77–113. (CABI: London)
María GA, Villarroel M, Sañudo C, Olleta JL, Gebresenbet G (2003) Effect of transport time and ageing on aspects of beef quality. Meat Science 65, 1335–1340.
| Effect of transport time and ageing on aspects of beef quality.Crossref | GoogleScholarGoogle Scholar |
Minka NS, Ayo JO (2007) Physiological responses of transported goats treated with ascorbic acid during the hot-dry season. Animal Science Journal 78, 164–172.
| Physiological responses of transported goats treated with ascorbic acid during the hot-dry season.Crossref | GoogleScholarGoogle Scholar |
Minka NS, Ayo JO (2009) Physiological responses of food animals to road transportation stress. African Journal of Biotechnology 8, 1–13.
Minka NS, Ayo OJ (2010) Serum biochemical activities and muscular soreness in transported goats administered with ascorbic acid during the hot-dry season. European Journal Translational Myology – Basic and Applied Myology 1(4), 193–203.
Minka NS, Ayo JO (2012) Assessment of thermal load on transported goats administered with ascorbic acid during the hot-dry conditions. International Journal of Biometeorology 56, 333–341.
| Assessment of thermal load on transported goats administered with ascorbic acid during the hot-dry conditions.Crossref | GoogleScholarGoogle Scholar |
Minka NS, Ayo JO, Sackey AKB, Adelaiye AB (2009) Assessment and scoring of stresses imposed on goats during handling, loading, road transportation and unloading, and the effect of pretreatment with ascorbic acid. Livestock Science 125, 275–282.
| Assessment and scoring of stresses imposed on goats during handling, loading, road transportation and unloading, and the effect of pretreatment with ascorbic acid.Crossref | GoogleScholarGoogle Scholar |
Miranda-de la Lama GC, Monge P, Villarroel M, Olleta JL, García-Belenguer S, María GA (2011) Effects of road type during transport on lamb welfare and meat quality in dry hot climates. Tropical Animal Health and Production 43, 915–922.
| Effects of road type during transport on lamb welfare and meat quality in dry hot climates.Crossref | GoogleScholarGoogle Scholar |
Mousaie A, Valizadeh R, Naserian AA, Heidarpour M, Mehrjerdi HK (2014) Impacts of feeding selenium–methionine and chromium–methionine on performance, serum components, antioxidant status, and physiological responses to transportation stress of Baluchi ewe lambs. Biological Trace Element Research 162, 113–123.
| Impacts of feeding selenium–methionine and chromium–methionine on performance, serum components, antioxidant status, and physiological responses to transportation stress of Baluchi ewe lambs.Crossref | GoogleScholarGoogle Scholar |
Nikbin S, Panandam JM, Sazili AQ (2016) Influence of pre-slaughter transportation and stocking density on carcass and meat quality characteristics of Boer goats. Italian Journal of Animal Science 15, 504–511.
| Influence of pre-slaughter transportation and stocking density on carcass and meat quality characteristics of Boer goats.Crossref | GoogleScholarGoogle Scholar |
Nwunuji TP, Mayowa OO, Yusoff SM, Bejo S-K, Salisi S, Mohd EAW (2014) The ameliorative effect of ascorbic acid on the oxidative status, live weight and recovery rate in road transport stressed goats in a hot humid tropical environment. Animal Science Journal 85, 611–616.
| The ameliorative effect of ascorbic acid on the oxidative status, live weight and recovery rate in road transport stressed goats in a hot humid tropical environment.Crossref | GoogleScholarGoogle Scholar |
Peel MC, Finlayson BL, McMahon TA (2007) Updated world map of the Köppen–Geiger climate classification. Hydrology and Earth System Sciences 11, 1633–1644.
| Updated world map of the Köppen–Geiger climate classification.Crossref | GoogleScholarGoogle Scholar |
Polycarp TN, Obukowho EB, Yusoff SM (2016) Changes in haematological parameters and oxidative stress response of goats subjected to road transport stress in a hot humid tropical environment. Comparative Clinical Pathology 25, 285–293.
| Changes in haematological parameters and oxidative stress response of goats subjected to road transport stress in a hot humid tropical environment.Crossref | GoogleScholarGoogle Scholar |
Rajion MA, Saat IM, Zulkifli I, Goh YM (2001) The effects of road transportation on some physiological stress measures in goats. Asian-Australasian Journal of Animal Sciences 14, 1250–1252.
| The effects of road transportation on some physiological stress measures in goats.Crossref | GoogleScholarGoogle Scholar |
Roe JH, Bailey JM, Gray RR, Robinson JN (1961) Complete removal of glycogen from tissues by extraction with cold trichloroacetic acid solution. The Journal of Biological Chemistry 236, 1244–1246.
Rucker RB, Morris J, Fascetti AJ (2008) Chapter 23. Vitamins. In ‘Clinical biochemistry of domestic animals’. 6th edn. (Eds JW Harvey, ML Bruss) pp. 695–730. (Academic Press: San Diego, CA)
Sanchez-Sanchez M, Vieira-Aller C, De-La-Fuente-Vazquez J, Perez-Marcos C, Lauzurica-Gomez S, Gonzalez-De-Chavarri E, Diaz-Diaz-Chiron MT (2013) Effect of season and stocking density during transport on carcass and meat quality of suckling lambs. Spanish Journal of Agricultural Research 11, 394–404.
| Effect of season and stocking density during transport on carcass and meat quality of suckling lambs.Crossref | GoogleScholarGoogle Scholar |
Wei T, Simko V (2017) ‘R package ‘corrplot’: visualization of a correlation matrix (version 0.84).’ Available at https://github.com/taiyun/corrplot [Verified 9 February 2018]
West JW (1994) Interactions of energy and bovine somatotropin with heat stress. Journal of Dairy Science 77, 2091–2102.
| Interactions of energy and bovine somatotropin with heat stress.Crossref | GoogleScholarGoogle Scholar |
Wierbicki E, Deatherage FE (1958) Water content of meats, determination of water-holding capacity of fresh meats. Journal of Agricultural and Food Chemistry 6, 387–392.
| Water content of meats, determination of water-holding capacity of fresh meats.Crossref | GoogleScholarGoogle Scholar |
Zhong RZ, Liu HW, Zhou DW, Sun HX, Zhao CS (2011) The effects of road transportation on physiological responses and meat quality in sheep differing in age. Journal of Animal Science 89, 3742–3751.
| The effects of road transportation on physiological responses and meat quality in sheep differing in age.Crossref | GoogleScholarGoogle Scholar |