Histomorphometric analysis of the small intestine of broiler chick embryos injected in ovo with methionine
Mohammad Naser Nazem A C , Sayed Mohsen Sajjadian A , Reza Kheirandish B and Hamideh Mohammadrezaei A
+ Author Affiliations
- Author Affiliations
A Department of Basic Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
B Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
C Corresponding author. Email: nnazem@uk.ac.ir
Animal Production Science 59(1) 133-139 https://doi.org/10.1071/AN17269
Submitted: 29 April 2017 Accepted: 16 September 2017 Published: 8 December 2017
Abstract
The present study evaluated the histomorphometric effect on the small intestine of the chicken embryo after in ovo methionine injection. On Day 4 of incubation, 50 fertile eggs were allocated into one of the following five groups: control (no treatment) and four treatment groups that received either 20, 30, 40 or 50 mg methionine via their yolk sac. All eggs were incubated until Day 19, at which point the embryos were terminated and 1-cm samples of the duodenum, jejunum and ileum were taken for histology. Sections were stained by haematoxylin and eosin, Alcian blue and periodic acid Schiff methods separately. Morphometric analysis was performed to assess goblet cell number, enterocyte height, muscle-layer thickness as well as villus height, width, area and shape. The ratio of embryo bodyweight to egg weight in methionine treatment groups was more than in controls and this difference was greatest in the 40-mg methionine group. The results showed that villous height, width and area increased in treatment groups, as did enterocyte height, goblet cell number and muscle-layer thickness. The ratio and sequence of the villi was also changed in some treatments. Our results indicated that injecting methionine into the yolk sac can improve intestinal histomorphometrical parameters and that 40-mg methionine injection showed the greatest changes.
Additional keywords: embryogenesis, methionine, poultry, protein, sulfur, supplementation.
References
Al-Murrani WK (1978) Maternal effects on embryonic and postembryonic growth in poultry. British Poultry Science 19, 277–281.| Maternal effects on embryonic and postembryonic growth in poultry.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaE1c7ms1OgsA%3D%3D&md5=62a5dc0c3c2251c9b164991c6740a98cCAS |
Al-Murrani WK (1982) Effect of injecting amino acids into the egg on embryonic and subsequent growth in the domestic fowl. British Poultry Science 23, 171–174.
| Effect of injecting amino acids into the egg on embryonic and subsequent growth in the domestic fowl.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL38XitFKmsLo%3D&md5=6dd9e0d182ffcaac0cbd4059eb3f1e1cCAS |
Aptekmann KP, Baraldi ASM, Stefanini MA, Orsi MA (2001) Morphometric analysis of the intestine of domestic quails (Coturnix Coturnix japonica) treated with different levels of dietary calcium. Anatomia, Histologia, Embryologia 30, 277–280.
| Morphometric analysis of the intestine of domestic quails (Coturnix Coturnix japonica) treated with different levels of dietary calcium.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3Mnjs1WmsA%3D%3D&md5=1b48b4ab40d01ec90e60e89685d636c1CAS |
Bartell SM, Batal AB (2007) The effect of supplemental glutamine on growth performance, development of the gastrointestinal tract and humoral immune response of broilers. Poultry Science 86, 1940–1947.
| The effect of supplemental glutamine on growth performance, development of the gastrointestinal tract and humoral immune response of broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVygu7vM&md5=3d78fae53ade54a411b024035f0c2176CAS |
Bhanja SK, Mandal AB (2005) Effect of in ovo injection of critical amino acids on pre and post hatch growth, immunocompetence and development of digestive organs in broiler chickens. AJAS 18, 524–531.
Bhanja SK, Mandal AB, Goswami TK (2004) Effect of in ovo injection of amino acid on growth immune response, development of digestive organs and carcass yield of broiler. Indian Journal of Poultry Science 39, 212–218.
Cebra JJ (1999) Influences of microbiota on intestinal immune system development. The American Journal of Clinical Nutrition 69, 1046S–1051S.
Christensen VL, Grimes JL, Donaldson WE, Lerner S (2000) Correlation of body weight with hatchling blood glucose concentration and its relationship to embryonic survival. Poultry Science 79, 1817–1822.
| Correlation of body weight with hatchling blood glucose concentration and its relationship to embryonic survival.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXitV2qs7s%3D&md5=5fb1ef79081ccd7e0c5bf21a8df637f1CAS |
Coulombre AJ, Coulombre JL (1958) Intestinal development: I. Morphogenesis of the villi and musculature. Journal of Embryology and Experimental Morphology 6, 403–411.
Dunsford BR, Knabe DA, Haensly WE (1989) Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig. Journal of Animal Science 67, 1855–1863.
| Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL1MzlvV2nuw%3D%3D&md5=0e2acc0a57b5ce04e61df268d8b744a4CAS |
Escande F, Aubert JP, Porchet N, Buisine MP (2001) Human mucin gene MUC5AC: organization of its 5ʹ-region and central repetitive region. The Biochemical Journal 358, 763–772.
| Human mucin gene MUC5AC: organization of its 5ʹ-region and central repetitive region.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXntlSgsL4%3D&md5=178297fb4a92a7da67d9e1cda60cf7f2CAS |
Foye OT, Uni Z, Ferket PR (2006) Effect of in ovo feeding egg white protein, β-hydroxy-β-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poultry Science 85, 1185–1192.
| Effect of in ovo feeding egg white protein, β-hydroxy-β-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XntVyksb4%3D&md5=ed1602e7f4c7187e8b18fd953f31bf3cCAS |
Geyra A, Uni Z, Sklan D (2001a) Enterocyte dynamics and mucosal development in the posthatch chick. Poultry Science 80, 776–782.
| Enterocyte dynamics and mucosal development in the posthatch chick.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38%2Fhs1yjsg%3D%3D&md5=2fb3d9faebba9d663470a1903e08ea57CAS |
Geyra A, Uni Z, Sklan D (2001b) The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick. British Journal of Nutrition 86, 53–61.
| The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlsVOjtbg%3D&md5=912be523a6043d35f0c778faefebf575CAS |
Havenstein GB, Ferket PR, Qureshi MA (2003) Carcass composition and yield of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poultry Science 82, 1509–1518.
| Carcass composition and yield of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3sritlKgug%3D%3D&md5=23472019de6cefaa5cf697268c39cbf8CAS |
Kadam MM, Bhanja SK, Manda AB, Thakur R, Vasan P, Bhattacharyya A, Tyagi JS (2008) Effect of in ovo threonine supplementation on early growth, immunological responses and digestive enzyme activities in broiler chickens. British Poultry Science 49, 736–741.
| Effect of in ovo threonine supplementation on early growth, immunological responses and digestive enzyme activities in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsFaht7bE&md5=c9c873074eec702bfa18f37075482435CAS |
Mohammadrezaei H, Nazem MN, Mohammadrezaei M (2015) Effect of in ovo injection of methionine on the histomorphometry of jejunum of chicken embryo. BFIJ 7, 23–26.
Nazem MN, Kheirandish R, Babaei H, Dehghan F (2017) Effect of short-term administration of methionine on the ovary and uterus in a rat. Comparative Clinical Pathology
| Effect of short-term administration of methionine on the ovary and uterus in a rat.Crossref | GoogleScholarGoogle Scholar |
Ohta Y, Kidd MT (2001) Optimum site for in ovo amino acid injection in broiler breeder eggs. Poultry Science 80, 1425–1429.
| Optimum site for in ovo amino acid injection in broiler breeder eggs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnvVChu7Y%3D&md5=3a9836260d9f5ccf05bf8daac3dbfa30CAS |
Ohta Y, Tsushima N, Koide K, Kidd MT, Ishibashi T (1999) Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks. Poultry Science 78, 1493–1498.
| Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnsFSlsrs%3D&md5=a05cee984185d8f87ef6ca8cc6888c2dCAS |
Ohta Y, Kidd MT, Ishibashi T (2001) Embryo growth and amino acid concentration profiles of broiler breeder eggs, embryos and chicks after in ovo administration of amino acids. Poultry Science 80, 1430–1436.
| Embryo growth and amino acid concentration profiles of broiler breeder eggs, embryos and chicks after in ovo administration of amino acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnvVChu7c%3D&md5=612ef0b7b0d4260801b714a3f50aa2c5CAS |
Perez-Vilar J, Hill RL (1999) The structure and assembly of secreted mucins. The Journal of Biological Chemistry 274, 31751–31754.
| The structure and assembly of secreted mucins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnt12jsrg%3D&md5=f2227dc02beae043104786c6a643f2d2CAS |
Powell JJ, Jugdaohsingh R, Thompson RPH (1999) The regulation of mineral absorption in the gastrointestinal tract. The Proceedings of the Nutrition Society 58, 147–153.
| The regulation of mineral absorption in the gastrointestinal tract.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXjtlCht70%3D&md5=8a239d332d2526f60c59ade8a120e089CAS |
Satchithanandam S, Vargofcak-Apker M, Calvert RJ, Leeds AR, Cassidy MM (1990) Alteration of gastrointestinal mucin by fiber feeding in rats. The Journal of Nutrition 120, 1179–1184.
Sklan D (2001) Development of the digestive tract of poultry. World’s Poultry Science Journal 57, 415–428.
| Development of the digestive tract of poultry.Crossref | GoogleScholarGoogle Scholar |
Smirnov A, Sklan D, Uni Z (2004) Mucin dynamics in the chick small intestine are altered by starvation. The Journal of Nutrition 134, 736–742.
Turck D, Feste AS, Lifschitz CH (1993) Age and diet affect the composition of porcine colonic mucins. Pediatric Research 33, 564–567.
| Age and diet affect the composition of porcine colonic mucins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXks1yrtrY%3D&md5=fd0e5f97a81340953e6be0e75158e082CAS |
Uni Z, Ferket PR (2004) Methods for early nutrition and their potential. World’s Poultry Science Journal 60, 101–111.
| Methods for early nutrition and their potential.Crossref | GoogleScholarGoogle Scholar |
Uni Z, Ganot S, Sklan D (1998) Posthatch development of mucosal function in the broiler small intestine. Poultry Science 77, 75–82.
| Posthatch development of mucosal function in the broiler small intestine.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1c7ivFKmtQ%3D%3D&md5=64ce5a6fe5d8d3ec42d281fc313fa4b5CAS |
Uni Z, Noy Y, Sklan D (1999) Posthatch development of small intestinal function in the poult. Poultry Science 78, 215–222.
| Posthatch development of small intestinal function in the poult.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1M7lvVehsw%3D%3D&md5=64f6ac611736947ea8d568ddf0c3131eCAS |
Uni Z, Geyra A, Ben-Hur H, Sklan D (2000) Small intestinal development in the young chick: crypt formation and enterocyte proliferation and migration. British Poultry Science 41, 544–551.
| Small intestinal development in the young chick: crypt formation and enterocyte proliferation and migration.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M7pt1Sitg%3D%3D&md5=e6f974588f0f9e8edfaec53191d0ee5cCAS |
Uni Z, Smirnov A, Sklan D (2003a) Pre- and post-hatch development of goblet cells in the broiler small intestine: effect of delayed access to feed. Poultry Science 82, 320–327.
| Pre- and post-hatch development of goblet cells in the broiler small intestine: effect of delayed access to feed.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3s7gs12gtQ%3D%3D&md5=d700cd81b530b5f59abf6d4ba2b43ac3CAS |
Uni Z, Tako E, Gal-Garber O, Sklan D (2003b) Morphological, molecular and functional changes in the chicken small intestine of the late-term embryo. Poultry Science 82, 1747–1754.
| Morphological, molecular and functional changes in the chicken small intestine of the late-term embryo.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpvFygsLk%3D&md5=ab9d12611171a120566ee90fd08ee773CAS |
Uni Z, Ferket PR, Tako E, Kendar O (2005) In ovo feeding improves energy status of late-term chicken embryos. Poultry Science 84, 764–770.
| In ovo feeding improves energy status of late-term chicken embryos.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXktF2iur0%3D&md5=3ae039521201843deaae6c8733d4650eCAS |
Vaezi G, Teshfam M, Bahadoran S, Farazyan H, Hosseini S (2011) Effects of different levels of lysine on small intestinal villous morphology in starter diet of broiler chickens. Global Veterinaria 7, 523–526.
Watanabe T, Ohara S, Ichikawa T, Saigenji K, Hotta K (1996) Mechanisms for cytoprotection by vitamin U from ethanol-induced gastric mucosal damage in rats. Digestive Diseases and Sciences 41, 49–54.
| Mechanisms for cytoprotection by vitamin U from ethanol-induced gastric mucosal damage in rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xhs1Sru7k%3D&md5=01d9723bfde12ebe2a53ff56a3d78339CAS |
Watanabe T, Ohara S, Miyazawa S, Saigenji K, Hotta K (2000) Augmentative effects of L-cysteine and methylmethionine sulfonium chloride on mucin secretion in rabbit gastric mucous cells. Journal of Gastroenterology and Hepatology 15, 45–52.
| Augmentative effects of L-cysteine and methylmethionine sulfonium chloride on mucin secretion in rabbit gastric mucous cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1Smsr8%3D&md5=77ca95a22d4e385f8960069bd73200e2CAS |
Wilson JH (1991) Bone strength of caged layers as affected by dietary calcium and phosphorus concentrations, reconditioning, and ash content. British Poultry Science 32, 501–508.
| Bone strength of caged layers as affected by dietary calcium and phosphorus concentrations, reconditioning, and ash content.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3MzntFWgug%3D%3D&md5=6294818cee4e06f4dca7ea8218631a9eCAS |
Yagi T, Miyawaki Y, Nishikawa A, Horiyama S, Yamuchi K, Kuwano S (1990) Prostaglandin E2-mediated stimulation of mucus synthesis and secretion by rhein anthone, the active metabolite of sennosides A and B, in mouse colon. Journal of Pharmacy and Pharmacology 42, 542–545.
| Prostaglandin E2-mediated stimulation of mucus synthesis and secretion by rhein anthone, the active metabolite of sennosides A and B, in mouse colon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXhslertg%3D%3D&md5=e5933382eaf705890a311ae3075827e2CAS |
Yamauchi K, Isshiki Y (1991) Scanning electron microscopic observations on the intestine villi in growing White Leghorn and broiler chickens from 1 to 30 days of age. British Poultry Science 32, 67–78.
| Scanning electron microscopic observations on the intestine villi in growing White Leghorn and broiler chickens from 1 to 30 days of age.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3M3ms1SlsA%3D%3D&md5=f302a8d896d3df6652e9b3e67bef1099CAS |
Yamauchi K, Hida S, Isshiki Y (1992) Post-hatching developmental changes in the ultrastructure of the duodenal absorptive epithelial cell in 1, 10 and 60 d-old chickens, with special reference to mitochondria. British Poultry Science 33, 475–488.
| Post-hatching developmental changes in the ultrastructure of the duodenal absorptive epithelial cell in 1, 10 and 60 d-old chickens, with special reference to mitochondria.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38zltlynsQ%3D%3D&md5=e6cd30732fabb462d49b72eb9a82d4c6CAS |
