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Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Application of visible and near-infrared spectroscopy for evaluation of ewes milk with different feeds

A. Bahri A B , S. Nawar C E , H. Selmi D , M. Amraoui A , H. Rouissi A and A. M. Mouazen C E
+ Author Affiliations
- Author Affiliations

A Department of Animal Production, Higher School of Agriculture of Mateur, 7030 Mateur, Tunisia.

B National Agronomy Institute Tunis, 43 Avenue Charles Nicolle, Tunis 1082.

C Department of Environment, Ghent University, Coupure links 653, 9000 Gent, Belgium.

D Sylvo-Pastoral Institute of Tabarka, University of Jandouba, BP.n°345, Tabarka 8110, Tunisia.

E Corresponding author. Email: said.nawar@ugent.be; abdul.mouazen@ugent.be

Animal Production Science 59(6) 1190-1200 https://doi.org/10.1071/AN17240
Submitted: 19 April 2017  Accepted: 29 May 2018   Published: 1 August 2018

Abstract

Rapid measurement optical techniques have the advantage over traditional methods of being faster and non-destructive. In this work visible and near-infrared spectroscopy (vis-NIRS) was used to investigate differences between measured values of key milk properties (e.g. fat, protein and lactose) in 30 samples of ewes milk according to three feed systems; faba beans, field peas and control diet. A mobile fibre-optic vis-NIR spectrophotometer (350–2500 nm) was used to collect reflectance spectra from milk samples. Principal component analysis was used to explore differences between milk samples according to the feed supplied, and a partial least-squares regression and random forest regression were adopted to develop calibration models for the prediction of milk properties. Results of the principal component analysis showed clear separation between the three groups of milk samples according to the diet of the ewes throughout the lactation period. Milk fat, protein and lactose were predicted with good accuracy by means of partial least-squares regression (R2 = 0.70–0.83 and ratio of prediction deviation, which is the ratio of standard deviation to root mean square error of prediction = 1.85–2.44). However, the best prediction results were obtained with random forest regression models (R2 = 0.86–0.90; ratio of prediction deviation = 2.73–3.26). The adoption of the vis-NIRS coupled with multivariate modelling tools can be recommended for exploring to differences between milk samples according to different feed systems, and to predict key milk properties, based particularly on the random forest regression modelling technique.

Additional keywords: chemometrics, feeding, random forest.


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