Effect of reduced dietary non-phytate phosphorus concentration, with or without calcium reduction, in diets supplemented with phytase for weaned piglets from 28 to 63 days of age
A. N. Sélos A , J. L. Donzele B , R. F. M. O. Donzele B , T. R. Castro A , E. F. Cardoso C D , L. N. Rennó B and D. A. M. Braga BA School of Veterinary Medicine and Animal Science, Universidade Federal do Tocantins, BR-153, Km 112, s/n, Campus Araguaína, Araguaína, Tocantins, 77804-970, Brazil.
B Department of Animal Science, Universidade Federal de Viçosa, Peter Henry Rolfs Avenue, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
C Instituto Federal de Educação, Ciência e Tecnologia Baiano (Campus Itaberaba), Rio Branco Avenue, s/n, Centro, Itaberaba, Bahia, 46880-000, Brazil.
D Corresponding author. Email: evandro.cardoso@ifbaiano.edu.br
Animal Production Science 59(12) 2236-2244 https://doi.org/10.1071/AN17150
Submitted: 14 March 2017 Accepted: 9 April 2019 Published: 20 September 2019
Abstract
The aim of this study was to investigate the impact of reduced dietary non-phytate phosphorus (nPP) or nPP and calcium (Ca) concentrations in diets supplemented with phytase on performance, bone mineralisation and serum parameters of weaned piglets from 28 to 63 days of age. A total of 168 barrows and gilts, weaned at 28 days of age, with an average initial bodyweight of 8.11 ± 0.61 kg were randomly allocated in a completely randomised block design. Treatments consisted of a positive control and an additional six treatments, in a 3 × 2 factorial arrangement, totalling seven treatments (Trt). The positive control (PC) diet was formulated to meet or exceed the nutrient requirements of pigs weighing 8–30 kg bodyweight, without phytase supplementation, and the six other diets were formulated similar to the PC, except with a reduction in the nPP concentration of 0.065, 0.130 or 0.195% (Phase Feeding 1 (PF1), PF2 or PF3 respectively) or similar to PF1, PF2 and PF3 minus 0.16% Ca, supplemented with 2000 FTU/kg of phytase, with eight replicate pens/Trt of three piglets each, in a three-phase feeding program. From Day 28 to Day 35, the average daily gain was higher (P < 0.05) and the feed conversion rate was lower (P < 0.05) respectively for piglets fed the PC when compared with those fed PF3 and PC treatment minus 0.160% calcium and minus 0.195% nPP (PF6). In contrast, when evaluating the period from Day 28 to 49, piglets fed PC presented the highest average daily gain, but only differed (P < 0.05) to those fed PF3 and PF6. When considering the overall period, at 63 days of age, no significant difference was detected (P > 0.05) between the PF Trts tested and the PC regarding performance. As for bone mineralisation at 63 days, bone P (BP) decreased as nPP in the diet decreased, regardless of the Ca level. However, except for PF3 and PF6, BP and third metacarpal bone ash concentration were respectively higher for pigs fed the PF Trts, when compared with PC. All treatments containing phytase resulted in a lower bone Ca : BP ratio when compared with PC. Serum P concentrations decreased as nPP in the diet decreased, regardless of the level of dietary Ca. However, with the exception of PF3 and PF6, serum P was not significantly different than pigs fed the PC. In conclusion, reducing nPP or nPP and Ca in diets containing 2000 FTU Ronozyme® HiPhos/kg phytase had no adverse effects on growth performance and increased BP and third metacarpal bone ash concentration compared with pigs fed a nutritionally adequate control diet.
Additional keywords: animal production, feed utilisation, microbial phytase, mineral nutrition, pig nutrition.
References
Alebrante L, Donzele JL, Oliveira RFM, Saraiva A, Guimarães SEF, Ferreira AS (2011) Available phosphorus levels in diets for pigs with high genetic potential for lean meat deposition kept in thermoneutral environment from 15 to 30 kg. Revista Brasileira de Zootecnia 40, 323–330.| Available phosphorus levels in diets for pigs with high genetic potential for lean meat deposition kept in thermoneutral environment from 15 to 30 kg.Crossref | GoogleScholarGoogle Scholar |
AOAC (2000) ‘Official methods of analysis.’ 17th edn. (Association of Official Analytical Chemists: Arlington, VA)
Bedford MR (2000) Exogenous enzymes in monogastric nutrition – their current value and future benefits. Animal Feed Science and Technology 86, 1–13.
| Exogenous enzymes in monogastric nutrition – their current value and future benefits.Crossref | GoogleScholarGoogle Scholar |
Bertol TM, Ludke JV (1999) Determinação dos valores de energia e do balanço de nitrogênio de alguns alimentos para leitões na fase inicial. Revista Brasileira de Zootecnia 28, 1279–1287.
| Determinação dos valores de energia e do balanço de nitrogênio de alguns alimentos para leitões na fase inicial.Crossref | GoogleScholarGoogle Scholar |
Boyd RD, Hall D, Wu JF (1983) Plasma alkaline phosphatase as a criterion for determining biological availability of phosphorus for swine. Journal of Animal Science 57, 396–401.
| Plasma alkaline phosphatase as a criterion for determining biological availability of phosphorus for swine.Crossref | GoogleScholarGoogle Scholar | 6619015PubMed |
Braña DV, Ellis M, Castañeda EO, Sands JS, Baker DH (2006) Effect of a novel phytase on growth performance, bone ash, and mineral digestibility in nursery and grower-finisher pigs. Journal of Animal Science 84, 1839–1849.
| Effect of a novel phytase on growth performance, bone ash, and mineral digestibility in nursery and grower-finisher pigs.Crossref | GoogleScholarGoogle Scholar | 16775068PubMed |
BRASIL (2000) Ministério da Agricultura. Instrução Normativa no. 3, de 17 de janeiro de 2000. Regulamento técnico de métodos de insensibilização para o abate humanitário de animais de açougue. Diário Oficial da União, Distrito Federal, Brasília, Brazil, pp. 14–16.
Buffington DE, Colazzo-Arocho A, Canton GH, Pitt D, Thatcher WW, Collier RJ (1981) Black globe-humidity index (BGHI) as comfort equation for dairy cows. Transactions of the ASAE. American Society of Agricultural Engineers 24, 711–714.
| Black globe-humidity index (BGHI) as comfort equation for dairy cows.Crossref | GoogleScholarGoogle Scholar |
Colégio Brasileiro de Experimentação Animal (COBEA) (1991) Princípios éticos na experimentação animal. In ‘Congresso do Colégio Brasileiro de Experimentação Animal’. (COBEA: São Paulo)
Columbus D, Niven SJ, Zhu CL, Lange CFM (2010) Phosphorus utilization in starter pigs fed high-moisture corn-based liquid diets steeped with phytase. Journal of Animal Science 88, 3964–3976.
| Phosphorus utilization in starter pigs fed high-moisture corn-based liquid diets steeped with phytase.Crossref | GoogleScholarGoogle Scholar | 20729280PubMed |
Cowieson AJ, Wilcock P, Bedford MR (2011) Super-dosing effects of phytase in poultry and other monogastrics. World’s Poultry Science Journal 67, 225–236.
| Super-dosing effects of phytase in poultry and other monogastrics.Crossref | GoogleScholarGoogle Scholar |
Dantas WMF, Filho JDR, Guimarães JD, Guimarães SEF (2014) Metabolic profile and body weight of piglets fed with different levels of phosphorus in distinct thermal environments. Pesquisa Agropecuária Brasileira 49, 901–910.
| Metabolic profile and body weight of piglets fed with different levels of phosphorus in distinct thermal environments.Crossref | GoogleScholarGoogle Scholar |
Doige CE, Mills JHL, Owen BD (1975) Influence of calcium and phosphorus on growth and skeletal development of growing swine. Canadian Journal of Animal Science 55, 147–164.
| Influence of calcium and phosphorus on growth and skeletal development of growing swine.Crossref | GoogleScholarGoogle Scholar |
Koch ME, Mahan DC, Corley JR (1984) An evaluation of various biological characteristics in assessing low phosphorus intake in weanling swine. Journal of Animal Science 59, 1546–1556.
| An evaluation of various biological characteristics in assessing low phosphorus intake in weanling swine.Crossref | GoogleScholarGoogle Scholar | 6526759PubMed |
Létourneau-Montminy MP, Narcy A, Maqnin M, Sauvant D, Bernier JF, Pomar C, Jondreville C (2010) Effect of reduced dietary calcium concentration and phytase supplementation on calcium and phosphorus utilization in weanling pigs with modified mineral status. Journal of Animal Science 88, 1706–1717.
| Effect of reduced dietary calcium concentration and phytase supplementation on calcium and phosphorus utilization in weanling pigs with modified mineral status.Crossref | GoogleScholarGoogle Scholar | 20118415PubMed |
Létourneau-Montminy MP, Narcy A, Lescoat P, Magnin M, Bernier JF, Sauvant D, Jondreville C, Pomar C (2011) Modeling the fate of dietary phosphorus in the digestive tract of growing pigs. Journal of Animal Science 89, 3596–3611.
| Modeling the fate of dietary phosphorus in the digestive tract of growing pigs.Crossref | GoogleScholarGoogle Scholar | 21680789PubMed |
Liu J, Bollinger DW, Ledoux DR, Veum TL (1998) Lowering the dietary calcium to total phosphorus ratio increases phosphorus utilization in low-phosphorus corn-soybean meal diets supplemented with microbial phytase for growing-finishing pigs. Journal of Animal Science 76, 808–813.
| Lowering the dietary calcium to total phosphorus ratio increases phosphorus utilization in low-phosphorus corn-soybean meal diets supplemented with microbial phytase for growing-finishing pigs.Crossref | GoogleScholarGoogle Scholar | 9535341PubMed |
Ludke MCMM, Lópes J, Ludke JV (2002) Phytase in diets for growing pigs: (i) environmental impact. Ciência Rural 32, 97–102.
| Phytase in diets for growing pigs: (i) environmental impact.Crossref | GoogleScholarGoogle Scholar |
Mahan DC (1982) Dietary calcium and phosphorus levels for weanling swine. Journal of Animal Science 54, 559–564.
| Dietary calcium and phosphorus levels for weanling swine.Crossref | GoogleScholarGoogle Scholar | 7085515PubMed |
Pond WG, Church DC, Pond KR (1995) ‘Basic animal nutrition and feeding.’ 4th edn. (John Wiley & Sons: New York, NY)
Qian H, Kornegay ET, Conner DE (1996) Adverse effects of wide calcium : phosphorus ratios on supplemental phytase efficacy for weanling pigs fed two dietary phosphorus levels. Journal of Animal Science 74, 1288–1297.
| Adverse effects of wide calcium : phosphorus ratios on supplemental phytase efficacy for weanling pigs fed two dietary phosphorus levels.Crossref | GoogleScholarGoogle Scholar | 8791201PubMed |
Reinhart GA, Mahan DC (1986) Effect of various calcium : phosphorus ratios at low and high dietary phosphorus for starter, grower and finishing swine. Journal of Animal Science 63, 457–466.
| Effect of various calcium : phosphorus ratios at low and high dietary phosphorus for starter, grower and finishing swine.Crossref | GoogleScholarGoogle Scholar |
Rostagno HS, Albino LFT, Donzele JL, Gomes PC, Oliveira RF, Lopes DC, Ferreira AS, Barreto SLT, Euclides RF (Eds) (2011) ‘Tabelas brasileira para aves e suínos: composição de alimentos e exigências nutricionais.’ 3rd edn. (Editora UFV: Viçosa, MG, Brazil)
SAS Institute Inc. (2001) ‘Statistical analysis software.’ 8.2. (CDROM) (SAS Institute Inc.: Cary, NC)
Selle PH, Ravindran V, Cowieson AJ, Bedford MR (2001) Phytate and Phytase. In ‘Enzymes in farm animal nutrition.’ 2nd edn. (Eds MR Bedford, GG Partridge) pp. 160–205. (CABI Publishing: New York, NY)
Selle PH, Cowieson AJ, Ravindran V (2009) Consequences of calcium interactions with phytate and phytase for poultry and pigs. Livestock Science 124, 126–141.
| Consequences of calcium interactions with phytate and phytase for poultry and pigs.Crossref | GoogleScholarGoogle Scholar |
Sethi PK, McMurtry JP, Pesti GM, Edwards HM, Aggrey SE (2008) Physiological responses to divergent selection for phytate phosphorus bioavailability in a random bred chicken population. Poultry Science 87, 2512–2516.
| Physiological responses to divergent selection for phytate phosphorus bioavailability in a random bred chicken population.Crossref | GoogleScholarGoogle Scholar | 19038807PubMed |
Shelton JL, Southern LL, LeMieux FM, Bidner TD, Page TG (2004) Effects of microbial phytase, low calcium and phosphorus, and removing the dietary trace mineral premix on carcass traits, pork quality, plasma metabolites, and tissue mineral content in growing-finishing pigs. Journal of Animal Science 82, 2630–2639.
| Effects of microbial phytase, low calcium and phosphorus, and removing the dietary trace mineral premix on carcass traits, pork quality, plasma metabolites, and tissue mineral content in growing-finishing pigs.Crossref | GoogleScholarGoogle Scholar | 15446480PubMed |
Simons PC, Versteegh HA, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MG, Beudeker RF, Verschoor GJ (1990) Improvement of phosphorus availability by microbial phytase in broilers and pigs. British Journal of Nutrition 64, 525–540.
| Improvement of phosphorus availability by microbial phytase in broilers and pigs.Crossref | GoogleScholarGoogle Scholar | 2171634PubMed |
Underwood EJ (1969) ‘Los minerals en la alimentación del ganado.’ (Acribia: Zaragoza, Spain).
Vargas JG, Albino LFT, Rostagno HS, Gomes PC, Cupertinno ES, Carvalho DCO, Nascimento AH (2003) Nutritional levels of calcium and available phosphorus for white-egg pullets and brown-egg pullets from 0 to 6 weeks of age. Revista Brasileira de Zootecnia 32, 1919–1926.
| Nutritional levels of calcium and available phosphorus for white-egg pullets and brown-egg pullets from 0 to 6 weeks of age.Crossref | GoogleScholarGoogle Scholar |
Varley PF, Callan JJ, O’Doherty JV (2011a) Effect of dietary phosphorus and calcium level and phytase addition on performance, bone parameters, apparent nutrient digestibility, mineral and nitrogen utilization of weaner pigs and the subsequent effect on finisher pig bone parameters. Animal Feed Science and Technology 165, 201–209.
| Effect of dietary phosphorus and calcium level and phytase addition on performance, bone parameters, apparent nutrient digestibility, mineral and nitrogen utilization of weaner pigs and the subsequent effect on finisher pig bone parameters.Crossref | GoogleScholarGoogle Scholar |
Varley PF, Flynn B, Callan JJ, O’Doherty JV (2011b) Effect of phytase level in a low phosphorus diet on performance and bone development in weaner pigs and the subsequent effect on finisher pig bone development. Livestock Science 138, 152–158.
| Effect of phytase level in a low phosphorus diet on performance and bone development in weaner pigs and the subsequent effect on finisher pig bone development.Crossref | GoogleScholarGoogle Scholar |
Zeng ZK, Piao XS, Wang D, Li PF, Xue LF, Salmon L, Zhang HY, Han X, Liu L (2011) Effect of microbial phytase on performance, nutrient absorption and excretion in weaned pigs and apparent ileal nutrient digestibility in growing pigs. Asian-Australasian Journal of Animal Sciences 24, 1164–1172.
| Effect of microbial phytase on performance, nutrient absorption and excretion in weaned pigs and apparent ileal nutrient digestibility in growing pigs.Crossref | GoogleScholarGoogle Scholar |
Zeng ZK, Wang D, Piao XS, Li PF, Zhang HY, Shi CX, Yu SK (2014) Effects of adding super dose phytase to the phosphorus-deficient diets of young pigs on growth performance, bone quality, minerals and amino acids digestibilities. Asian-Australasian Journal of Animal Sciences 27, 237–246.
| Effects of adding super dose phytase to the phosphorus-deficient diets of young pigs on growth performance, bone quality, minerals and amino acids digestibilities.Crossref | GoogleScholarGoogle Scholar | 25049948PubMed |
