Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Nitrate is safe to feed ad libitum in molasses roller drums as a source of non-protein nitrogen

J. P. Goopy A B C and R. S. Hegarty B
+ Author Affiliations
- Author Affiliations

A International Livestock Research Institute, Nairobi, Kenya.

B School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.

C Corresponding author. Email: j.goopy@cgiar.org

Animal Production Science 59(7) 1288-1292 https://doi.org/10.1071/AN16824
Submitted: 19 December 2016  Accepted: 20 July 2018   Published: 10 December 2018

Abstract

We investigated voluntary intake, growth and safety of cattle offered low-quality forage diets plus isonitrogenous molasses-based liquid supplements containing either urea (U) or a calcium nitrate-containing compound (NO3). We hypothesised that changing the nitrogen source from U to calcium nitrate would not jeopardise animal health or affect intake. Angus cattle (n = 24) were allocated to six pens, with three pens each receiving a molasses supplement containing U or a molasses supplement containing NO3 for 31 days. There was a trend (P = 0.06) for the NO3 treatment group to consume more of the (oaten chaff) basal diet than the U treatment group. The U group consumed more supplement than did the NO3 group (1.31 vs 0.40 kg DM/head.day s.e.m. = 0.094, P < 0.0001), but total DM intake was not different (6.45 vs 6.10 kg/head.day, P = 0.15). Mean final animal liveweight was not different between treatments. Methaemoglobin levels were higher in the NO3 group (2.1 vs 1.3%, P < 0.001). Low consumption of nitrate was also reflected in there being no effect of nitrate on the methane production rate when assessed in open-circuit calorimetry chambers (7.1 vs 7.0 g/head.2 h, P = 0.898). It is confirmed that nitrate may be safely provided to cattle when dissolved at 154 g/kg in a molasses-based liquid supplement available ad libitum, but may not be an effective methane mitigant due to low NO3 intake. It is speculated that nitrate may be a useful tool to limit voluntary intake of non-protein nitrogen supplements.

Additional keywords: enteric methane, supplementation.


References

Bruning-Fann CS, Kaneene J (1993) The effects of nitrate, nitrite and N-nitroso compounds on human health: a review. Veterinary and Human Toxicology 35, 521–538.

Clark JL, Pfander WH, Bloomfield RA, Krause GF, Thompson GB (1970) Nitrate containing rations for cattle supplemented with either urea or soybean meal. Journal of Animal Science 31, 961–966.
Nitrate containing rations for cattle supplemented with either urea or soybean meal.Crossref | GoogleScholarGoogle Scholar |

de Raphélis-Soissan V Li L Godwin IR Barnett MC Perdok H Hegarty RS 2014 Use of nitrate and Propionibacterium acidipropionici to reduce methane emissions and increase wool growth of Merino sheep. Animal Production Science 54 1860 1866

de Raphélis-Soissan V, Nolan JV, Godwin IR, Newbold JR, Perdok HB, Hegarty RS (2017) Paraffin-wax-coated nitrate salt inhibits short-term methane production in sheep and reduces the risk of nitrite toxicity. Animal Feed Science and Technology 229, 57–64.
Paraffin-wax-coated nitrate salt inhibits short-term methane production in sheep and reduces the risk of nitrite toxicity.Crossref | GoogleScholarGoogle Scholar |

Entwistle K, Knights G (1974) The use of urea-molasses supplements for sheep grazing semi-arid tropical pastures. Australian Journal of Experimental Agriculture 14, 17–22.
The use of urea-molasses supplements for sheep grazing semi-arid tropical pastures.Crossref | GoogleScholarGoogle Scholar |

Goopy JP (2007) Physiological bases for divergent methane production in ruminants. PhD Thesis, University of New England, Armidale.

Goopy J, Hegarty R, Robinson D (2009) Two hour chamber measurement provides a useful estimate of daily methane production in sheep. In ‘Ruminant physiology: digestion, metabolism and effects of nutrition on reproduction and welfare; proceedings of the XIth international symposium on ruminant physiology’, 6–9 September 2009, Clermont-Ferrand, France. (Wageningen Academic Publishers)

GraphPad (2012) ‘GraphPad Prism.’ (La Jolla, CA)

Hegarty RS, Robinson DL, Li DL, Oelbrandt L, Luijben N, Mc K, Grath J, Bremner G, Perdok HB (2013) Growth, efficiency and carcass attributes of feedlot cattle supplemented with calcium nitrate or urea. Advances in Animal Science 4, 440

Hegarty R, Bird S, Woodgate R (2014) Cattle respiration facility, Armidale, New South Wales, Australia. In ‘Technical manual on respiration chamber designs’. (Eds C Pinares, G Waghorn) pp. 29–41. (Ministry of Agriculture and Forestry: Wellington, New Zealand)

Herd RM, Arthur PF, Donoghue KA, Bird SH, Bird-Gardiner T, Hegarty RS (2014) Measures of methane production and their phenotypic relationships with dry matter intake, growth, and body composition traits in beef cattle. Journal of Animal Science 92, 5267–5274.
Measures of methane production and their phenotypic relationships with dry matter intake, growth, and body composition traits in beef cattle.Crossref | GoogleScholarGoogle Scholar |

Lee C, Beauchemin KA (2014) A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions, and production performance. Canadian Journal of Animal Science 94, 557–570.
A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions, and production performance.Crossref | GoogleScholarGoogle Scholar |

Leng RA (1990) Factors affecting the utilization of ‘poor-quality’ forages by ruminants particularly under tropical conditions. Nutrition Research Reviews 3, 277–303.
Factors affecting the utilization of ‘poor-quality’ forages by ruminants particularly under tropical conditions.Crossref | GoogleScholarGoogle Scholar |

Leng R, Preston T (2010) Further considerations of the potential of nitrate as a high affinity electron acceptor to lower enteric methane production in ruminants. Livestock Research for Rural Development 22, 221

Li L, Davis J, Nolan J, Hegarty R (2012) An initial investigation on rumen fermentation pattern and methane emission of sheep offered diets containing urea or nitrate as the nitrogen source. Animal Production Science 52, 653–658.

Li L, Silveira CI, Nolan JV, Godwin IR, Leng RA, Hegarty RS (2013) Effect of added dietary nitrate and elemental sulfur on wool growth and methane emission of Merino lambs. Animal Production Science 53, 1195–1201.
Effect of added dietary nitrate and elemental sulfur on wool growth and methane emission of Merino lambs.Crossref | GoogleScholarGoogle Scholar |

McLennan SR, Hirst DJ, Shepherd RK, McGuigan KR (1991) A comparison of various methods of feeding supplements of urea, sulfur and molasses to weaner heifers during the dry season in northern Queensland. Australian Journal of Experimental Agriculture 31, 153–158.
A comparison of various methods of feeding supplements of urea, sulfur and molasses to weaner heifers during the dry season in northern Queensland.Crossref | GoogleScholarGoogle Scholar |

Newbold JR, van Zijderveld SM, Hulshof RBA, Fokkink WB, Leng RA, Terencio P, Powers WJ, van iewed at:Adrichem PSJ, Paton ND, Perdok HB (2014) The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. Journal of Animal Science 92, 5032–5040.
The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls.Crossref | GoogleScholarGoogle Scholar |

NGGI (2014) Quarterly update of Australia’s National Greenhouse Gas Inventory: June 2014. Available at https://www.environment.gov.au/system/files/resources/2bd59b0d-cf8f-4bdf-8e23-5250e4361c24/files/nggi-quarterly-update-june-2014_0.pdf [Verified 3 September 2018]

Nguyen SH, Hegarty RS (2016) Effects of defaunation and dietary coconut oil distillate on fermentation, digesta kinetics and methane production of Brahman heifers. Journal of Animal Physiology and Animal Nutrition 101, 984–993.
Effects of defaunation and dietary coconut oil distillate on fermentation, digesta kinetics and methane production of Brahman heifers.Crossref | GoogleScholarGoogle Scholar |

Nguyen SH, Bremner G, Cameron M, Hegarty RS (2016) Methane emissions, ruminal characteristics and nitrogen utilisation changes after refaunation of protozoa-free sheep. Small Ruminant Research 144, 48–55.
Methane emissions, ruminal characteristics and nitrogen utilisation changes after refaunation of protozoa-free sheep.Crossref | GoogleScholarGoogle Scholar |

Nolan JV, Norton BW, Murray RM, Ball FM, Roseby FB, Rohan-Jones W, Hill MK, Leng RA (1975) Body weight and wool production in grazing sheep given access to a supplement of urea and molasses: intake of supplement/response relationships. The Journal of Agricultural Science 84, 39–48.
Body weight and wool production in grazing sheep given access to a supplement of urea and molasses: intake of supplement/response relationships.Crossref | GoogleScholarGoogle Scholar |

Nolan JV, Hegarty RS, Hegarty J, Godwin IR, Woodgate R (2010) Effects of dietary nitrate on fermentation, methane production and digesta kinetics in sheep. Animal Production Science 50, 801–806.
Effects of dietary nitrate on fermentation, methane production and digesta kinetics in sheep.Crossref | GoogleScholarGoogle Scholar |

Nolan JV, Godwin IR, de Raphélis-Soissan V, Hegarty RS (2016) Managing the rumen to limit the incidence and severity of nitrite poisoning in nitrate-supplemented ruminants. Animal Production Science 56, 1317–1329.
Managing the rumen to limit the incidence and severity of nitrite poisoning in nitrate-supplemented ruminants.Crossref | GoogleScholarGoogle Scholar |

van Zijderveld SM, Gerrits WJJ, Dijkstra J, Newbold JR, Hulshof RBA, Perdok HB (2011) Persistency of methane mitigation by dietary nitrate supplementation in dairy cows. Journal of Dairy Science 94, 4028–4038.
Persistency of methane mitigation by dietary nitrate supplementation in dairy cows.Crossref | GoogleScholarGoogle Scholar |

Velazco J, Hegarty RS (2014) Feeding behaviour needs to be considered in validating methane mitigation strategies for feedlot cattle using short-term measurements of methane emissions. Poster presentation, international symposium on ruminant physiology, 8–12 September 2014, Canberra, Australia.

Velazco JI, Cottle DJ, Hegarty RS (2014) Methane emissions and feeding behaviour of feedlot cattle supplemented with nitrate or urea. Animal Production Science 54, 1737–1740.
Methane emissions and feeding behaviour of feedlot cattle supplemented with nitrate or urea.Crossref | GoogleScholarGoogle Scholar |