Production responses of reproducing ewes to a by-product-based diet inoculated with the probiotic Bacillus amyloliquefaciens strain H57
Oanh T. Le A , Benjamin Schofield B , Peter J. Dart B , Matthew J. Callaghan C , Allan T. Lisle B , Diane Ouwerkerk D , Athol V. Klieve B and David M. McNeill A E
+ Author Affiliations
- Author Affiliations
A School of Veterinary Science, The University of Queensland, Gatton, Qld 4343, Australia.
B School of Agriculture and Food Sciences, The University of Queensland, Gatton, Qld 4343, Australia.
C Ridley AgriProducts Pty Ltd, Toowong, Qld 4066, Australia.
D Department of Agriculture, Fisheries and Forestry, Dutton Park, Qld 4102, Australia.
E Corresponding author. Email: d.mcneill@uq.edu.au
Animal Production Science 57(6) 1097-1105 https://doi.org/10.1071/AN16068
Submitted: 4 September 2015 Accepted: 8 March 2016 Published: 19 August 2016
Abstract
The potential application of the spore-forming probiotic Bacillus amyloliquefaciens strain H57 (H57) as a novel probiotic for ruminants was evaluated in reproducing ewes. Performance responses were determined by delivering H57 in a pelleted diet based mainly on palm kernel meal (PKM) and sorghum grain. PKM is an agro-industrial by-product with a reputation for poor palatability and the availability of the starch in sorghum grain can be limited in ruminants. The hypothesis was that H57 improves the feeding value of a relatively low quality concentrate diet. Twenty-four first-parity white Dorper ewes were fed PKM-based pellets manufactured with or without H57 (109 cfu/kg pellet) in late pregnancy. During this phase of late pregnancy, the H57 ewes ate 17% more dry matter (1019 vs 874 g/day, P = 0.03), gained more weight (194 vs 30 g/day, P = 0.008) and retained more nitrogen (6.13 vs 3.34 g/day, P = 0.01), but produced lambs with a similar birthweight (4.1 vs 4.2 kg, P = 0.73). Rumen fluid collected from H57 ewes in late pregnancy had higher pH (7.1 vs 6.8, P = 0.07), acetate : propionate ratio (3.4 vs 2.7, P = 0.04), lower ammonia (69 vs 147 mmol/L, P = 0.001) and total volatile fatty acid concentrations (40 vs 61 mg/L, P = 0.02). The digestibility of dry matter, organic matter and fibre were similar between the two groups. The lambs of the H57 ewes grew faster than those of the Control ewes for the first 21 days of lactation (349 vs 272 g/day, P = 0.03), but not thereafter. H57 can improve feed intake and maternal liveweight gain in late pregnancy of first-parity ewes fed a diet based on PKM.
Additional keywords: feed intake, liveweight, palm kernel meal, pregnant ewes.
References
Adams MC, Luoa J, Rayward D, King S, Gibson R, Moghaddam GH (2008) Selection of a novel direct-fed microbial to enhance weight gain in intensively reared calves. Animal Feed Science and Technology 145, 41–52.| Selection of a novel direct-fed microbial to enhance weight gain in intensively reared calves.Crossref | GoogleScholarGoogle Scholar |
Alexopoulos C, Karagiannidis A, Kritas SK, Boscos C, Georgoulakis IE, Kyriakis SC (2001) Field evaluation of a bioregulator containing live Bacillus cereus spores on health status and performance of sows and their Litters. Journal of Veterinary Medicine Serries A 48, 137–145.
| Field evaluation of a bioregulator containing live Bacillus cereus spores on health status and performance of sows and their Litters.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M3psVSjsg%3D%3D&md5=3a78b905eb02ff2be185255297f47dbbCAS |
Alimon AR, Ivan M, Jalaludin S (2011) Effects of different levels of dietary sulfur and molybdenum on concentrations of copper and other elements in plasma and liver of lambs fed palm kernel cake diets. British Journal of Nutrition 106, 1224–1230.
| Effects of different levels of dietary sulfur and molybdenum on concentrations of copper and other elements in plasma and liver of lambs fed palm kernel cake diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlGns7%2FF&md5=c61b77cdd629e441ea6f1e42633aed00CAS | 21492494PubMed |
Antunovi Z, Šperanda M, Amidž D, Šeri V, Steiner Z, Domacinovi M, Boli F (2006) Probiotic application in lambs nutrition. Krmiva 48, 175–180.
AOAC (1990) ‘Official methods of analysis.’ (Association of Official Analytical Chemists: Washington, DC)
Bayatkouhsar J, Tahmasebi AM, Naserian AA, Mokarram RR, Valizadeh R (2013) Effects of supplementation of lactic acid bacteria on growth performance, blood metabolites and fecal coliform and lactobacilli of young dairy calves. Animal Feed Science and Technology 186, 1–11.
| Effects of supplementation of lactic acid bacteria on growth performance, blood metabolites and fecal coliform and lactobacilli of young dairy calves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXovVSqu70%3D&md5=98074b3c914ddd9aa32db7481faaf0efCAS |
Brown S, Dart P (2005) ‘Testing hay treated with mould-inhibiting, biocontrol inoculum.’ (Rural Industries Research and Development Corporation: Canberra)
Chanjula P, Siriwathananukul Y, Lawpetchara A (2011) Effect of feeding Rubber seed kernel and Palm kernel cake in combination on nutrient utilization, rumen fermentation characteristics, and microbial populations in goats fed on Briachiaria humidicola hay-based diets. Asian-Australasian Journal of Animal Sciences 24, 73–81.
| Effect of feeding Rubber seed kernel and Palm kernel cake in combination on nutrient utilization, rumen fermentation characteristics, and microbial populations in goats fed on Briachiaria humidicola hay-based diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjs12qsrs%3D&md5=31d9a48f3ffa22487e8781d5df36df81CAS |
Chaucheyras-Durand DF, Durand H (2010) Probiotics in animal nutrition and health. Beneficial Microbes 1, 3–9.
| Probiotics in animal nutrition and health.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFGru7rN&md5=e113d9370ec3ef7faf2a7c38ed6785dfCAS |
Chaucheyras-Durand F, Walker ND, Bach A (2008) Effects of active dry yeasts on the rumen microbial ecosystem: Past, present and future. Animal Feed Science and Technology 145, 5–26.
| Effects of active dry yeasts on the rumen microbial ecosystem: Past, present and future.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpsVKrtLo%3D&md5=8a7655a97f556e1b3c8d11bdefd47a7dCAS |
Chaucheyras-Durand F, Chevaux E, Martin C, Forano E (2012) Use of yeast probiotics in ruminants: Effects and mechanisms of action on rumen pH, fibre degradation, and microbiota according to the diet. In ‘Probiotic in animal’. (Ed. EC Rigobelo) pp. 119–152. (INTECH: Rijeka, Croatia)
Cottyn BG, Boucque CV (1968) Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry 16, 105–107.
| Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF1cXjs1Glsw%3D%3D&md5=31bb9a23dd5f458d864d3777f3a2759cCAS |
Dijkstra J, Forbes JM, Frances J (2005) ‘Quantitative aspects of ruminant digestion and metabolism.’ (CAB International: Wallingford, UK)
Freer M (2007) ‘Nutrient requirements of domesticated ruminants.’ (CSIRO Publishing: Melbourne)
Frizzo LS, Soto LP, Zbrun MV, Bertozzi E, Sequeira G, Rodríguez Armesto R, Rosmini MR (2010) Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder. Animal Feed Science and Technology 157, 159–167.
| Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXkvFKrsLw%3D&md5=22aff4d75e6907202d94e1a04c173eecCAS |
Frizzo LS, Zbrun MV, Soto LP, Signorini ML (2011) Effects of probiotics on growth performance in young calves: a meta-analysis of randomized controlled trials. Animal Feed Science and Technology 169, 147–156.
| Effects of probiotics on growth performance in young calves: a meta-analysis of randomized controlled trials.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1ensr3I&md5=ac117a57d37ab5a587c221649162e374CAS |
Galina MA, Ortiz-Rubio MA, Delgado-Pertinez M, Pineda LJ (2009) Goat kid’s growth improvement with a lactic probiotic fed on a standard base diet. Options Mesditerranéennes 85, 315–323.
Garza‐Cázares F, Daenicke R, Flachowsky G (2001) Research note: effect of Bacillus cereus on performances of growing bulls. Archiv fur Tierernahrung 55, 161–165.
| Research note: effect of Bacillus cereus on performances of growing bulls.Crossref | GoogleScholarGoogle Scholar | 12068483PubMed |
Hinch GN, Brien F (2014) Lamb survival in Australian flocks: a review. Animal Production Science 54, 656–666.
| Lamb survival in Australian flocks: a review.Crossref | GoogleScholarGoogle Scholar |
Hong HA, Duc LH, Cutting SM (2005) The use of bacterial spore formers as probiotics. FEMS Microbiology Reviews 29, 813–835.
| The use of bacterial spore formers as probiotics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXos1WktLs%3D&md5=fcbfbb02f550a85794625c7f9c4f26a7CAS | 16102604PubMed |
Jatkauskas J, Vrotniakiene V (2010) Effects of probiotic dietary supplementation on diarrhoea patterns, faecal microbiota and performance of early weaned calves. Veterinarni Medicina 55, 494–503.
Khalid MF, Muhammad S, Mahr-Un-Nisa , Zia-Ur-Rehman (2011) Response of growing lambs fed on different vegetable protein sources with or without probiotics. International Journal of Agriculture and Biology 13, 332–338.
Kowalski ZM, Górka P, Schlagheck A, Jagusiak W, Micek P, Strzetelski J (2009) Performance of Holstein calves fed milk-replacer and starter mixture supplemented with probiotic feed additive. Journal of Animal and Feed Sciences 18, 399–411.
Kristensen NB, Danfær A, Agergaard N (1998) Absorption and metabolism of short‐chain fatty acids in ruminants. Archiv fur Tierernahrung 51, 165–175.
| Absorption and metabolism of short‐chain fatty acids in ruminants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXksVWns74%3D&md5=cff8ca46f2578bbf4cee3387a8ca8e6aCAS | 9672714PubMed |
Kritas SK, Govaris A, Christodoulopoulos G, Burriel AR (2006) Effect of Bacillus licheniformis and Bacillus subtilis supplementation of ewe’s feed on sheep milk production and young lamb mortality. Journal of Veterinary Medicine A 53, 170–173.
| Effect of Bacillus licheniformis and Bacillus subtilis supplementation of ewe’s feed on sheep milk production and young lamb mortality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XlsFWqu7w%3D&md5=56aeb741698f2695b2910a40f38b0992CAS |
Kumagai H, Kumagae S, Mitani K, Endo T (2004) Effects of supplementary probiotics to two different diets on dry matter intake, daily gain, digestibility, ruminal pH, and fecal microbial populations and metabolites in ewes. Animal Science Journal 75, 219–224.
| Effects of supplementary probiotics to two different diets on dry matter intake, daily gain, digestibility, ruminal pH, and fecal microbial populations and metabolites in ewes.Crossref | GoogleScholarGoogle Scholar |
Malik R, Bandla S (2010) Effect of source and dose of probiotics and exogenous fibrolytic enzymes (EFE) on intake, feed efficiency, and growth of male buffalo (Bubalus bubalis) calves. Tropical Animal Health and Production 42, 1263–1269.
| Effect of source and dose of probiotics and exogenous fibrolytic enzymes (EFE) on intake, feed efficiency, and growth of male buffalo (Bubalus bubalis) calves.Crossref | GoogleScholarGoogle Scholar | 20401692PubMed |
Masucci F, De Rosa G, Grasso F, Napolitano F, Esposito G, Di Francia A (2011) Performance and immune response of buffalo calves supplemented with probiotic. Livestock Science 137, 24–30.
| Performance and immune response of buffalo calves supplemented with probiotic.Crossref | GoogleScholarGoogle Scholar |
McNeill D (2013) Forages for ruminants, cereals for human food and fue. In ‘Optimisation of feed use efficiency in ruminant production systems’. (Eds HPS Makkar, D Beever) pp. 15–32. (Food and Agriculture Organization of the United Nations and Asian-Australian Association of Animal Production Societies: Rome)
Mohan B, Kadirvel R, Natarajan A, Bhaskaran M (1996) Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers. British Poultry Science 37, 395–401.
| Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XjvFygtL0%3D&md5=983fdac2be8f94972d497648ada3e6edCAS | 8773848PubMed |
Newbold CJ (1996) Probiotics for ruminants. Annales de Zootechnie 45, 329–335.
| Probiotics for ruminants.Crossref | GoogleScholarGoogle Scholar |
Norton BW, Dart PJ, Brown SM (2008) The effects of microbial amendment of rye-grass clover hay at baling on intake and nutritive value for pregnant sheep. Proceding of Australian Social Animal Production 27, 110
Peng H, Wang JQ, Kang HY, Dong SH, Sun P, Bu DP, Zhou LY (2012) Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows. Journal of Animal Physiology and Animal Nutrition 96, 506–512.
| Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xpt12isLw%3D&md5=6c937d59b4896eb2f79711e0e616edddCAS | 21635575PubMed |
Pitt RE, Pell AN (1997) Modeling ruminal pH fluctuations: Interactions between meal frequency and digestion rate. Journal of Dairy Science 80, 2429–2441.
| Modeling ruminal pH fluctuations: Interactions between meal frequency and digestion rate.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXntFemsLc%3D&md5=9e29cee1522fb88bb8f59dbe5e2d15c1CAS | 9361215PubMed |
Playne MJ (1985) Determination of ethanol, volatile fatty acids, lactic and succinic acids in fermentation liquids by gas chromatography. Journal of the Science of Food and Agriculture 36, 638–644.
| Determination of ethanol, volatile fatty acids, lactic and succinic acids in fermentation liquids by gas chromatography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXmtFehtL8%3D&md5=5eff264df1c199893dff2c0dbb1e2a60CAS |
Qiao GH, Shan AS, Ma N, Ma QQ, Sun ZW (2010) Effect of supplemental Bacillus cultures on rumen fermentation and milk yield in Chinese Holstein cows. Journal of Animal Physiology and Animal Nutrition 94, 429–436.
Raeth-Knight ML, Linn JG, Jung HG (2007) Effect of direct-fed microbials on performance, diet digestibility, and rumen characteristics of Holstein dairy cows. Journal of Dairy Science 90, 1802–1809.
| Effect of direct-fed microbials on performance, diet digestibility, and rumen characteristics of Holstein dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjs1ahs70%3D&md5=92a894831c03b9e0d1e36408c48ddfa2CAS | 17369221PubMed |
Riddell JB, Gallegos AJ, Harmon DL, McLeod KR (2010) Addition of a Bacillus based probiotic to the diet of preruminant calves: influence on growth, health, and blood parameters. International Journal of Applied Research in Veterinary Medicine 8, 78–86.
Shen Z, Kuhla S, Zitnan R, Seyfert HM, Schneider F, Hagemeister H, Chudy A, Löhrke B, Blum JW, Hammon HM, Voigt J (2005) Intraruminal infusion of n-butyric acid induces an increase of ruminal papillae size independent of IGF-1 system in castrated bulls. Archives of Animal Nutrition 59, 213–225.
| Intraruminal infusion of n-butyric acid induces an increase of ruminal papillae size independent of IGF-1 system in castrated bulls.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtV2hurfI&md5=670149ecd6d27a2c187060c6aa596276CAS | 16320810PubMed |
Signorini ML, Soto LP, Zbrun MV, Sequeira GJ, Rosmini MR, Frizzo LS (2012) Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria. Research in Veterinary Science 93, 250–258.
| Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38zktlKmsQ%3D%3D&md5=2bf7a020cf140900842ca38c29c7828aCAS | 21620428PubMed |
Sun P, Wang JQ, Zhang HT (2010) Effects of Bacillus subtilis natto on performance and immune function of preweaning calves. Journal of Dairy Science 93, 5851–5855.
| Effects of Bacillus subtilis natto on performance and immune function of preweaning calves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjs1Kis7k%3D&md5=97e8dfbfda603b79460abb47d748949bCAS | 21094758PubMed |
Sun P, Wang JQ, Zhang HT (2011) Effects of supplementation of Bacillus subtilis natto Na and N1 strains on rumen development in dairy calves. Animal Feed Science and Technology 164, 154–160.
| Effects of supplementation of Bacillus subtilis natto Na and N1 strains on rumen development in dairy calves.Crossref | GoogleScholarGoogle Scholar |
Suttle NF (2010) ‘Mineral nutrition of livestock.’ (CAB International: Cambridge, MA; Wallingford, Oxfordshire, UK)
Thompson AN, Ferguson MB, Campbell AJD, Gordon DJ, Kearney GA, Oldham CM, Paganoni BL (2011) Improving the nutrition of Merino ewes during pregnancy and lactation increases weaning weight and survival of progeny but does not affect their mature size. Animal Production Science 51, 784–793.
| Improving the nutrition of Merino ewes during pregnancy and lactation increases weaning weight and survival of progeny but does not affect their mature size.Crossref | GoogleScholarGoogle Scholar |
Timmerman HM, Mulder L, Everts L, van Espen DC, van der Wal E, Klaassen G, Rouwers SMG, Hartemink R, Rombouts FM, Beynen AC (2005) Health and growth of veal calves fed milk replacers with or without probiotics. Journal of Dairy Science 88, 2154–2165.
| Health and growth of veal calves fed milk replacers with or without probiotics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXksFyrurk%3D&md5=d2bf3a57aea8290b1a646cf31d1f871aCAS | 15905445PubMed |
Underwood EJ, Suttle NF (1999) ‘The minerals nutrition of livestock.’ (CABI: Wallingford, UK)
Van Soet PJ (1989) ‘Nutritional ecology of the ruminant.’ 2nd edn. (Cornell University Press: New York, NY)
Weiß C (2007) StatSoft, Inc., Tulsa, OK: STATISTICA, version 8. AStA Advances in Statistical Analysis 91, 339–341.
