Effects of applying lactic acid bacteria and propionic acid on fermentation quality, aerobic stability and in vitro gas production of forage-based total mixed ration silage in Tibet
L. Chen A , X. J. Yuan A , J. F. Li A , Z. H. Dong A , S. R. Wang A , G. Guo B and T. Shao A C
+ Author Affiliations
- Author Affiliations
A Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, China.
B College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China.
C Corresponding author. Email: taoshaolan@163.com
Animal Production Science 59(2) 376-383 https://doi.org/10.1071/AN16062
Submitted: 3 February 2016 Accepted: 16 October 2017 Published: 10 January 2018
Abstract
Total mixed ration (TMR) silage technology has been practically used to feed ruminants in Tibet. This study was conducted on forage-based TMR to evaluate the effects of supplementing lactic acid bacteria and propionic acid on its fermentation characteristics, aerobic stability and in vitro gas production kinetics and digestibility. Experimental treatments included four variants: (1) TMR supplemented with 10 mL deionised water per kilogram fresh matter (Control); (2) TMR supplemented with 1 × 106 cfu/g Lactobacillus plantarum (L); (3) TMR supplemented with 0.3% propionic acid (P); (4) TMR supplemented with a combination of 1 × 106 cfu/g Lactobacillus plantarum and 0.3% propionic acid (LP). The latter three additives were first dissolved in deionised water and then applied as a water solution (10 mL/kg fresh matter). All treatments were ensiled in laboratory-scale silos for 45 days, and then exposed to air for 12 days to evaluate the aerobic stability of TMR silage. Further, the four experimental treatments were fermented with buffered rumen fluid to measure in vitro gas production and nutrients’ digestibility. The results indicated that all TMR silages possessed good fermentation characteristics with low pH values (<4.18) and ammonia nitrogen (NH3-N) contents (<100 g/kg total nitrogen), and high lactic acid contents (>66 g/kg DM) and Flieg points (>80). The addition of L and LP stimulated a more efficient homofermentation of TMR silage than in the variant without L, as evidenced by higher ratios of lactic : acetic acid. The addition of P had no effect (P > 0.05) on lactic acid production of TMR silage compared with the Control, whereas it decreased NH3-N content (P < 0.05). Under aerobic conditions, L silage showed less aerobic stability compared with the Control silage, whereas P and LP silages were more (P < 0.05) aerobically stable. Compared with the Control, all additives elevated (P < 0.05) the total gas production and in vitro dry matter digestibility of TMR silages. L silage had a higher (P < 0.05) in vitro neutral detergent fibre digestibility than the Control silage. Data obtained from this study suggested that TMR silage based on oat and common vetch can be well conserved with or without additives. Lactic acid bacteria were compatible with propionic acid, and addition of lactic acid bacteria together with propionic acid can improve the fermentation quality, aerobic stability and in vitro dry matter digestibility of TMR silage.
Additional keywords: additives, aerobic stability, fermentation characteristics, forage, in vitro gas production kinetics.
References
Adesogan AT, Salawu MB (2002) The effect of different additives on the fermentation quality, aerobic stability and in vitro digestibility of pea/wheat bi-crop silages containing contrasting pea to wheat ratios. Grass and Forage Science 57, 25–32.| The effect of different additives on the fermentation quality, aerobic stability and in vitro digestibility of pea/wheat bi-crop silages containing contrasting pea to wheat ratios.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XkvVansb8%3D&md5=82965db598130d2f52e422b925ffbdd2CAS |
Amer S, Hassanat F, Berthiaume R, Seguin P, Mustaf AF (2012) Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Animal Feed Science and Technology 177, 23–29.
| Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVyktrjM&md5=0ba879e124738f91cd6649f2fe27409eCAS |
AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official Analytical Chemists: Arlington, VA)
Ashbell G, Weinberg ZG, Hen Y, Filya I (2002) The effects of temperature on the aerobic stability of wheat and corn silages. Journal of Industrial Microbiology & Biotechnology 28, 261–263.
| The effects of temperature on the aerobic stability of wheat and corn silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xjs12ru70%3D&md5=0800afab45ba97382d0073c104ce5ab6CAS |
Blümmel M, Karsli A, Russell JR (2003) Influence of diet on growth yields of rumen micro-organisms in vitro and in vivo: influence on growth yield of variable carbon fluxes to fermentation products. British Journal of Nutrition 90, 625–634.
| Influence of diet on growth yields of rumen micro-organisms in vitro and in vivo: influence on growth yield of variable carbon fluxes to fermentation products.Crossref | GoogleScholarGoogle Scholar |
Chen L, Guo G, Yuan X, Zhang J, Li J, Shao T (2016) Effects of applying molasses, lactic acid bacteria and propionic acid on fermentation quality, aerobic stability and in vitro gas production of total mixed ration silage prepared with oat–common vetch intercrop on the Tibetan Plateau. Journal of the Science of Food and Agriculture 96, 1678–1685.
| Effects of applying molasses, lactic acid bacteria and propionic acid on fermentation quality, aerobic stability and in vitro gas production of total mixed ration silage prepared with oat–common vetch intercrop on the Tibetan Plateau.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtVWqtrjK&md5=a328f63c741d6bda6922df0198149711CAS |
Ertl P, Zebeli Q, Zollitsch W, Knaus W (2015) Feeding of by-products completely replaced cereals and pulses in dairy cows and enhanced edible feed conversion ratio. Journal of Dairy Science 98, 1225–1233.
| Feeding of by-products completely replaced cereals and pulses in dairy cows and enhanced edible feed conversion ratio.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXitVyksrfJ&md5=356a8f8adc97e329599689f7e7c79f32CAS |
Filya I (2003) The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. Journal of Dairy Science 86, 3575–3581.
| The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXptFajurY%3D&md5=261e3826a3bd6b0a03b9f613893688bfCAS |
Gai JP, Christie P, Cai XB, Fan JQ, Zhang JL, Feng G, Li XL (2009) Occurrence and distribution of arbuscular mycorrhizal fungal species in three types of grassland community of the Tibetan Plateau. Ecological Research 24, 1345–1350.
| Occurrence and distribution of arbuscular mycorrhizal fungal species in three types of grassland community of the Tibetan Plateau.Crossref | GoogleScholarGoogle Scholar |
Getachew G, Blummel M, Makkar HPS, Beck K (1998) In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Animal Feed Science and Technology 72, 261–281.
| In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjvVWgsb4%3D&md5=17e4821c98ef820413cfa79004284e8fCAS |
Han LY, Li J, Na RS, Yu Z, Zhou H (2015) Effect of two additives on the fermentation, in vitro digestibility and aerobic security of Sorghum–sudangrass hybrid silages. Grass and Forage Science 70, 185–194.
| Effect of two additives on the fermentation, in vitro digestibility and aerobic security of Sorghum–sudangrass hybrid silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhslamt7s%3D&md5=f4cc8d1bfe33cbb76893b1e8635a2267CAS |
Huber JT, Soejono M (1976) Organic acid treatment of high dry matter corn silage fed to lactating dairy cows. Journal of Dairy Science 59, 2063–2070.
| Organic acid treatment of high dry matter corn silage fed to lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXoslyqtg%3D%3D&md5=480ad2af453fc8c8728ebebc90d5bb98CAS |
Jaakkola S, Kaunisto V, Huhtanen P (2006) Volatile fatty acid proportions and microbial protein synthesis in the rumen of cattle receiving grass silage ensiled with different rates of formic acid. Grass and Forage Science 61, 282–292.
| Volatile fatty acid proportions and microbial protein synthesis in the rumen of cattle receiving grass silage ensiled with different rates of formic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFGitL3J&md5=b014f0e314f04d1ac9328948a92273eaCAS |
Kaiser AG, Dear BS, Morris SG (2007) An evaluation of the yield and quality of oat–legume and ryegrass–legume mixtures and legume monocultures harvested at three stages of growth for silage. Animal Production Science 47, 25–38.
| An evaluation of the yield and quality of oat–legume and ryegrass–legume mixtures and legume monocultures harvested at three stages of growth for silage.Crossref | GoogleScholarGoogle Scholar |
Keles G, Demirci U (2011) The effect of homofermentative and heterofermentative lactic acid bacteria on conservation characteristics of baled triticale-Hungarian vetch silage and lamb performance. Animal Feed Science and Technology 164, 21–28.
| The effect of homofermentative and heterofermentative lactic acid bacteria on conservation characteristics of baled triticale-Hungarian vetch silage and lamb performance.Crossref | GoogleScholarGoogle Scholar |
Kilic A (1986) ‘Silo feed (instruction, education and application proposals).’ (Bilgehan Press: Izmir, Turkey)
Kleinschmit DH, Schmidt RJ, Kung L (2005) The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. Journal of Dairy Science 88, 2130–2139.
| The effects of various antifungal additives on the fermentation and aerobic stability of corn silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXksFyrurs%3D&md5=a793f2a51fc05301b4addc6573e69a37CAS |
Kozelov LK, Lliev F, Hristov AN, Zaman S, McAllister TA (2008) Effect of fibrolytic enzyme and an inoculation in vitro degradability and gas production of low-dry matter alfalfa silage. Journal of the Science of Food and Agriculture 88, 2568–2575.
| Effect of fibrolytic enzyme and an inoculation in vitro degradability and gas production of low-dry matter alfalfa silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlansL%2FL&md5=3aee7fa4d84ac4169bc6da46e1dbd896CAS |
Kung L, Sheperd AC, Smagala AM, Endres KM, Bessett CA, Ranjit NK, Glancey JL (1998) The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. Journal of Dairy Science 81, 1322–1330.
| The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsVOqu7g%3D&md5=99791dff4754dc4910741b08342d3ad8CAS |
Kung L, Robinson JR, Ranjit NK, Chen JH, Golt CM, Pesek JD (2000) Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative. Journal of Dairy Science 83, 1479–1486.
| Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXltVGgtL0%3D&md5=fe0a0de28bd58587a0ffdc040894225bCAS |
Kung L, Taylor CC, Lynch MP, Neylon JM (2003) The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows. Journal of Dairy Science 86, 336–343.
| The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhtlCktrs%3D&md5=1d436b56738f2d133d603d71a91d2367CAS |
Lindgren S (1991) Microbial dynamics during silage fermentation. In ‘Grass and forage reports’. (Eds S Lindgren, KL Pettersson) pp. 135–145. (Presented at the Proceedings of the Eurobac Conference, Swedish University of Agricultural Sciences Publishers: Uppsala, Sweden)
Lithourgidis AS, Vasilakoglou IB, Dhima KV, Dordas CA, Yiakoulaki MD (2006) Forage yield and quality of common vetch mixtures with oat and triticale in two seeding ratios. Field Crops Research 99, 106–113.
| Forage yield and quality of common vetch mixtures with oat and triticale in two seeding ratios.Crossref | GoogleScholarGoogle Scholar |
McDonald P, Henderson AR, Heron SJE (1991) ‘The biochemistry of silage.’ (Chalcombe Publications: Marlow, UK)
McEniry J, O’Kiely P, Clipson NJW, Forristal PD, Doyle EM (2007) Manipulating the ensilage of wilted, unchopped grass through the use of additive treatments. Irish Journal of Agricultural and Food Research 46, 77–91.
Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 7–55.
Muck RE, O’Kiely P (1992) Aerobic deterioration of lucerne (Medicago sativa) and maize (Zea mais) silages-effects of fermentation products. Journal of the Science of Food and Agriculture 59, 145–149.
| Aerobic deterioration of lucerne (Medicago sativa) and maize (Zea mais) silages-effects of fermentation products.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XlsV2ku7k%3D&md5=eaf23cf04e5b916862115dfdfb0a4264CAS |
Muck RE, Filya I, Contreras-Govea FE (2007) Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production. Journal of Dairy Science 90, 5115–5125.
| Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1Kru7zI&md5=dbf0ee30aa2471f21b81c6e47184f394CAS |
Nishino N, Hattori H (2007) Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria. Journal of the Science of Food and Agriculture 87, 2420–2426.
| Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFygtbrL&md5=07ed6341827ca621d4170d2cc43ea0f8CAS |
Nkosi BD, Meeske R, Langa T, Motiang MD, Modiba S, Mkhize NR, Groenewald IB (2016) Effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion of the silage by Damara rams. Small Ruminant Research 134, 90–96.
| Effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion of the silage by Damara rams.Crossref | GoogleScholarGoogle Scholar |
Nsereko VL, Beauchemin KA, Morgavi DP, Rode LM, Furtado AF, McAllister TA, Iwaasa AD, Yang WZ, Wang Y (2002) Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Canadian Journal of Microbiology 48, 14–20.
| Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XitF2mu70%3D&md5=ad4dfebaf4d776c839fb96c29b559e06CAS |
Pahlow G, Muck RE, Driehuis F, Elferink SJWHO, Spoelstra SF (2003) Microbiology of ensiling. In ‘Silage science and technology’. (Eds DR Buxton, RE Muck, JH Harrison) pp. 31–93. (American Society of Agronomy, Crop Science Society of America, Soil Science Society of America Publishers: Madison, WI)
Patra AK, Yu Z (2013) Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro. Journal of Dairy Science 96, 1782–1792.
| Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXht1Oru7w%3D&md5=b72ebc5e2d481c30c1de543d582897b0CAS |
Pursiainen P, Tuori M (2008) Effect of ensiling field bean, field pea and common vetch in different proportions with whole-crop wheat using formic acid or an inoculant on fermentation characteristics. Grass and Forage Science 63, 60–78.
| Effect of ensiling field bean, field pea and common vetch in different proportions with whole-crop wheat using formic acid or an inoculant on fermentation characteristics.Crossref | GoogleScholarGoogle Scholar |
Ranjit NK, Kung L (2000) The effects of lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. Journal of Dairy Science 83, 526–535.
| The effects of lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1ymsLk%3D&md5=ac8072966779b47c6db91a06efe1a64bCAS |
Rebolé A, Alzueta C, Ortiz LT, Barro C, Rodríguez ML, Caballero R (2004) Yields and chemical composition of different parts of the common vetch at flowering and at two seed filling stages. Spanish Journal of Agricultural Research 2, 550–557.
| Yields and chemical composition of different parts of the common vetch at flowering and at two seed filling stages.Crossref | GoogleScholarGoogle Scholar |
Richard E, Heutte N, Bouchart V, Garon D (2009) Evaluation of fungal contamination and mycotoxin production in maize silage. Animal Feed Science and Technology 148, 309–320.
| Evaluation of fungal contamination and mycotoxin production in maize silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1SltQ%3D%3D&md5=fa31b97cb9469e7c9e0f9edf4b773831CAS |
Rymer C, Huntington JA, Williams BA, Givens DI (2005) In vitro cumulative gas production techniques: History, methodological considerations and challenges. Animal Feed Science and Technology 123–124, 9–30.
| In vitro cumulative gas production techniques: History, methodological considerations and challenges.Crossref | GoogleScholarGoogle Scholar |
SAS Inc. (2001) ‘SAS® user’s guide: statistics, version 8.2 edition.’ (SAS Inc.: Cary, NC)
Seppälä A, Heikkilä T, Mäki M, Miettinen H, Rinne M (2013) Controlling aerobic stability of grass silage-based total mixed rations. Animal Feed Science and Technology 179, 54–60.
| Controlling aerobic stability of grass silage-based total mixed rations.Crossref | GoogleScholarGoogle Scholar |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=1bbe217b400e9f7fff04f7f3e179f03aCAS |
Wang F, Nishino N (2008) Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration. Journal of Dairy Science 91, 2380–2387.
| Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmsVWmtbw%3D&md5=4001f54e55c802743b15414330a10213CAS |
Woolford Mk (1990) The detrimental effects of air on silage. The Journal of Applied Bacteriology 68, 101–116.
| The detrimental effects of air on silage.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3c3gt1GmtA%3D%3D&md5=d27e2f93f158d578d019a9e53b001ab2CAS |
Yunus M, Ohba N, Shimojo M, Furuse M, Masuda Y (2000) Effects of adding urea and molasses on napiergrass silage quality. Asian-Australasian Journal of Animal Sciences 13, 1542–1547.
| Effects of adding urea and molasses on napiergrass silage quality.Crossref | GoogleScholarGoogle Scholar |
