Effect of ghrelin and leptin receptors genes polymorphisms on production results and physicochemical characteristics of M. pectoralis superficialis in broiler chickens
U. Kaczor A D , K. Poltowicz B , M. Kucharski A , A. M. Sitarz A , J. Nowak B , D. Wojtysiak C and D. A. Zieba A
+ Author Affiliations
- Author Affiliations
A Department of Animal Biotechnology, University of Agriculture in Krakow, Redzina 1B, 30-248 Krakow, Poland.
B Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice near Krakow, Poland.
C Department of Animal Reproduction and Anatomy, University of Agriculture in Krakow, 30-059 Krakow, Poland.
D Corresponding author. Email: rzkaczor@cyf-kr.edu.pl
Animal Production Science 57(1) 42-50 https://doi.org/10.1071/AN15152
Submitted: 24 March 2015 Accepted: 1 September 2015 Published: 22 January 2016
Abstract
Ghrelin and leptin and their receptors GHSR and LEPR regulate food intake, the processes in adipose tissue, and the body’s energy homeostasis in mammals. The aim of the present study was to determine the effect of GHSR/Csp6I and LEPR/Bsh1236I polymorphisms on the meat production parameters of broiler chickens reared to 42 days of age. In 318 fast-growing Hubbard Flex and Ross 308 chickens, g.3051C > T substitution at the GHSR locus and a GGTCAA deletion at positions g.3407_3409del and g.3411_3413del were identified. The use of restriction enzyme Bsh1236I showed the presence of two transitions g.352C > T and g.427G > A in LEPR locus. The chickens were classified into four GHSR/Csp6I and into five LEPR/Bsh1236I diplotypes. GHSR and LEPR polymorphisms were found to influence final bodyweight, daily gain, dressing percentage without giblets, proportion of giblets and the quality characteristics of M. pectoralis superficialis. GHSR/Csp6I and LEPR/Bsh1236I had an effect on pH24 h (P < 0.05) and lightness (L*) of M. pectoralis superficialis (P < 0.05), whereas GHSR/Csp6I influenced shear force (P < 0.05) and thawing loss (P < 0.05). GHSR/Csp6I and LEPR/Bsh1236I were found to have no effect on the abdominal fat content in chicken carcasses. Single nucleotide polymorphisms reported in the present study could be used in breeding programs as selection markers for growth traits and poultry meat quality.
Additional keywords: GHSR, LEPR, meat quality.
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