Polymorphisms of kappa-casein gene and their effects on milk production traits in Holstein, Jersey and Brown Swiss cattle*
Murad Gurses A E , Huseyin Yuce B , Ebru Onalan Etem C and Bahri Patir D
+ Author Affiliations
- Author Affiliations
A Balikesir University Faculty of Veterinary Medicine Department of Genetics, 10145 Balikesir, Turkey.
B Duzce University Faculty of Medicine Department of Medical Biology, 81000 Duzce, Turkey.
C Firat University Faculty of Medicine Department of Medical Biology, 23119 Elazig, Turkey.
D Firat University Faculty of Veterinary Medicine Department of Food Hygiene and Tecnology, 23119 Elazig, Turkey.
E Corresponding author. Email: mgurses@balikesir.edu.tr
Animal Production Science 58(5) 778-784 https://doi.org/10.1071/AN15131
Submitted: 11 March 2015 Accepted: 1 October 2016 Published: 2 December 2016
Abstract
The objectives of this study were to detect kappa-casein (κ-CN, CSN3, CASK) gene polymorphisms and to investigate the association between the κ-CN gene polymorphisms and milk yield and composition in cattle. For this purpose, a total of 365 blood and milk samples were collected from Holstein, Jersey and Brown Swiss cows. Polymorphisms of κ-CN gene were detected by the PCR and restriction fragment length polymorphism. A 776-bp fragment of exon IV was amplified by PCR and digested with HindIII and HaeIII restriction endonuclease to distinguish allele A, B and E. The analysis of associations between κ-CN genotypes and milk yield and composition were conducted with the use of the general linear model procedure. As a result, allele frequencies were detected as 0.736, 0.357, 0.308 for A allele, 0.186, 0.643, 0.692 for B allele in Holstein, Brown Swiss and Jersey breeds, respectively, and 0.079 for E allele in Holstein. This study also demonstrated the presence of E allele in Turkish Holstein. Although κ-CN genotypes were associated with protein and solids-not-fat content of milk in Hostein and Brown Swiss, they were associated with milk fat content in Jerseys (P < 0.05).
Additional keywords: Bovine CSN3, genetic polymorphism, milk yield and composition, PCR-RFLP.
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