Dietary phosphate equivalence of four forms of Pi contrasted with a novel microbial phytase from Citrobacter braakii in broiler chickens
A. J. Cowieson A C , F. Fru-Nji A and O. Adeola BA DSM Nutritional Products, Wurmisweg 576, 4303 Kaiseraugst, Switzerland.
B Department of Animal Sciences, Purdue University, West Lafayette, 47907 IN, USA.
C Corresponding author. Email: aaron.cowieson@dsm.com
Animal Production Science 55(9) 1145-1151 https://doi.org/10.1071/AN14489
Submitted: 8 April 2014 Accepted: 6 June 2014 Published: 15 September 2014
Abstract
Two 21-day studies were conducted with broilers to evaluate the efficacy of a bacterial 6-phytase from Citrobacter braakii and compare four Pi sources. The four sources were phosphates of monocalcium (MCP), dicalcium (DCP), tricalcium (TCP) with potassium phosphate (KH2PO4) acting as a ‘positive control’ reference. In each study, 336 4-day-old male birds (Ross 708) were blocked based on initial bodyweight (BW) and randomly allotted to one of seven diets with six replicate cages of eight birds each. Access to experimental diets and water from Days 4 to 25 post-hatching was ad libitum. In the first study, the seven diets were: (1) a low-P negative control (NC) corn-soybean meal basal diet formulated to contain crude protein (CP), Ca, total P, and non-phytate P (nPP) at 218, 9.0, 4.5, and 2.0 g/kg, respectively; (2) NC plus 0.75 g Pi from KH2PO4/kg; (3) NC plus 0.75 g Pi from MCP/kg; (4) NC plus 0.75 g Pi from DCP/kg; (5) NC plus 0.75 g Pi from TCP/kg; (6) NC plus phytase at 500 FYT/kg; (7) NC plus phytase at 1000 FYT/kg. Feeding the low-P NC diet reduced (P < 0.01) BW gain (BWG), feed intake (FI), and tibia ash. Supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, and tibia ash. Supplementing the NC with 0.75 g/kg Pi from MCP, DCP, or TCP were equipotent in improving BWG and FI; however, percent tibia ash was higher (P < 0.05) in birds fed MCP than either DCP or TCP. The second study was similar to the first study except that Ca, total P, and nPP in the NC were reduced to 7.0, 4.2, and 1.8 g/kg, respectively; and Pi from MCP, DCP, and TCP were reduced to 0.6 g/kg. Similar to observations in the first study, the low-P NC diet reduced (P < 0.01) BWG, FI, and tibia ash; and supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, P digestibility and tibia ash. Furthermore, supplementing the NC with 0.75 g Pi from MCP, DCP, or TCP per kg diet were equipotent in improving BWG, FI, and tibia ash though MCP resulted in superior (P < 0.05) retention of P compared with TCP. Results from both studies showed that the phytase was efficacious in releasing phytate-P for growth and bone mineralisation in chickens compensating, at least, the spared Pi, and that Pi sources commonly used in formulating diets of chickens may be different in their potential to supply digestible P.
Additional keywords: bone ash, broiler chickens, phytase, phosphate sources, phosphorus.
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