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RESEARCH ARTICLE

Effect of calcium salt of fatty acids supplementation on performance of Malpura lambs

R. S. Bhatt A B , A. Sahoo A , A. K. Shinde A and S. A. Karim A
+ Author Affiliations
- Author Affiliations

A Division of Animal Nutrition, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan 304501, India.

B Corresponding author. Email: bhatt_rs@yahoo.com

Animal Production Science 55(9) 1123-1130 https://doi.org/10.1071/AN13253
Submitted: 21 November 2012  Accepted: 18 January 2014   Published: 30 September 2014

Abstract

Twenty-four Malpura lambs (28 days old) were distributed into three equal groups (four each from either sexes). They were fed ad libitum on three experimental diets containing graded levels of calcium salt of fatty acids (source: rice bran oil; Ca-FA) at 0 (T1), 20 (T2) and 40 (T3) g per kg of concentrate and dry roughage (Prosopis cineraria leaves) up to 6 months of age along with suckling of mother till weaning (3 months). Effect of Ca-FA supplementation on liveweight gain, feed and nutrient intake was not observed during pre-weaning (28–90 days), which however increased (P < 0.05) in T3 during post-weaning (90–180 days). There was an increase in organic matter intake and digestibility as well as metabolisable energy intake in Ca-FA-supplemented groups. Rumen ammonia-N and ciliate protozoa population was increased (P < 0.05) in T2 and T3 with a positive shift in rumen fermentation (increased propionate and decreased acetate). Blood biochemical analysis revealed effect of treatment and age (P < 0.05) for free fatty acids and cholesterol concentration. Ca-FA supplementation improved (P < 0.05) dressing percentage both at 2% and 4% CA-FA inclusions levels with no change in fat yield and composition. It is concluded that supplementation of Ca-FA prepared from industrial-grade rice bran oil in growing lamb rations improved gain, organic matter intake and digestibility during the post-weaning period and a linear response to the level of supplementation recommends Ca-FA at 40 g/kg for improved lamb performance.


References

AAC (1990) ‘Feeding standards for Australian livestock.’ (Ruminant Sub Committee, Australian Agriculture Council, CSIRO Publishing: Melbourne)

Alexander G, Prabhakara Rao Z, Rama Prasad J (2002) Effect of sunflower acid oil or its calcium soap on rumen fermentation in sheep. Indian Journal of Animal Nutrition 19, 251–255.

AOAC (2000) ‘Official methods of analysis. Vol. 1 and 2.’ 17th edn. (Association of Official Analytical Chemists: Gaithersburg, MD)

Bhatt RS, Tripathi MK, Verma DL, Karim SA (2009) Effect of different feeding regimes on pre- weaning growth, rumen fermentation and its effect on post weaning performance of lambs. Journal of Animal Physiology and Animal Nutrition 93, 568–576.
Effect of different feeding regimes on pre- weaning growth, rumen fermentation and its effect on post weaning performance of lambs.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1MnivF2itg%3D%3D&md5=6d0b5e8fea1c6ad759ab6630b3fa9536CAS | 19141102PubMed |

Bhatt RS, Soren NM, Tripathi MK, Karim SA (2011) Effect of different levels of coconut oil supplementation on performance, digestibility, rumen fermentation and carcass traits of Malpura lambs. Animal Feed Science and Technology 164, 29–37.
Effect of different levels of coconut oil supplementation on performance, digestibility, rumen fermentation and carcass traits of Malpura lambs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhvFWlsbo%3D&md5=3aa9de5c24448fe2687959bd4268f00dCAS |

Bhatt RS, Soren NM, Sahoo A, Karim SA (2012) Re-alimentation strategy to maneuver body condition and carcass characteristics in cull ewes. Animal 6, 61–69.
Re-alimentation strategy to maneuver body condition and carcass characteristics in cull ewes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38votlGmsQ%3D%3D&md5=a66427459811eeee302aa6557d4aba67CAS | 22436155PubMed |

Bhatt RS, Sahoo A, Shinde AK, Karim SA (2013) Change in body condition and carcass characteristics of cull ewes fed diets supplemented with rumen bypass fat. Livestock Science 157, 132–140.
Change in body condition and carcass characteristics of cull ewes fed diets supplemented with rumen bypass fat.Crossref | GoogleScholarGoogle Scholar |

Bhatta R, Shinde AK, Vaithiyanathan S, Sankhyan SK, Verma DL (2002) Effect of polyethylene glycol-6000 on nutrient intake, digestion and growth of kids browsing Prosopis cineraria. Animal Feed Science and Technology 101, 45–54.

Bindel DJ, Drouillard JS, Titgemeyer EC, Wessels RH, Loest CA (2000) Effects of ruminally protected choline and dietary fat on performance and blood metabolites of finishing heifers. Journal of Animal Science 78, 2497–2503.

Butterfield RM (1988) ‘New concepts of sheep growth.’ (University of Sydney: Sydney)

Calsamiglia S, Cardozo PW, Ferret A, Bach A (2007) Changes in rumen microbial fermentation are due to a combined effect of type of diet and pH. Journal of Animal Science 86, 702–711.
Changes in rumen microbial fermentation are due to a combined effect of type of diet and pH.Crossref | GoogleScholarGoogle Scholar | 18073289PubMed |

Chilliard Y (1993) Dietary fat and adipose tissue metabolism in ruminants, pigs, and rodents: a review. Journal of Dairy Science 76, 3897–3931.
Dietary fat and adipose tissue metabolism in ruminants, pigs, and rodents: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXhtlartLs%3D&md5=c2fa8f66012010eb0e168d11d7233f32CAS | 8132894PubMed |

Clinquart A, Micol D, Brundseaux C, Dufrasne I, Istasse L (1995) Utilisation des matierres grasses chez les bovines avec a I’engraissement. INRA. Productions Animales 8, 29–42.

Doreau M, Chilliard Y, Bauchart , D , Michalet-Doreau , B (1991) Influence of different fat supplements on digestibility and ruminal digestion in cows. Annales de Zootechnie 40, 19–30.
Influence of different fat supplements on digestibility and ruminal digestion in cows.Crossref | GoogleScholarGoogle Scholar |

Doumas BT (1975) Standards for total serum protein assays – a collaborative study. Clinical Chemistry 21, 1159–1166.

Fébel H, Husvéth , F , Veresegyházy T, Andrásofszky , E , Várhegyi I , Huszár , S (2002) Effect of different fat sources on in vitro degradation of nutrients and certain blood parameters in sheep. Acta Veterinaria Hungarica 50, 217–229.
Effect of different fat sources on in vitro degradation of nutrients and certain blood parameters in sheep.Crossref | GoogleScholarGoogle Scholar | 12113177PubMed |

Fiorentini G, Messana JD, Dian PHM, Reis RA, Canesin RC, Pires AV, Berchielli TT (2013) Digestibility, fermentation and rumen microbiota of crossbred heifers fed diets with different soybean oil availabilities in the rumen. Animal Feed Science and Technology 181, 26–34.
Digestibility, fermentation and rumen microbiota of crossbred heifers fed diets with different soybean oil availabilities in the rumen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXivVKitr4%3D&md5=71069a2a8567da7cca0d9c149e29879fCAS |

Grewal RS, Singla M, Lamba JS, Ahuja CS, Tyagi N (2013) Effect of diets varying in energy source on the growth and blood biochemical profile of Beetal kids under stall fed conditions. The Indian Journal of Animal Sciences 83, 290–294.

Gulati SK, Garg MR, Scott TW (2005) Rumen protected protein and fat produced from oilseeds and/or meals by formaldehyde treatment; their role in ruminant production and product quality: a review. Australian Journal of Experimental Agriculture 45, 1189–1203.
Rumen protected protein and fat produced from oilseeds and/or meals by formaldehyde treatment; their role in ruminant production and product quality: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1Squ7jE&md5=dd31766fd75fddbd73e1c36563845d8eCAS |

Haddad SG, Younis HM (2004) The effect of adding ruminally protected fat in fattening diets on nutrient intake, digestibility and growth performance of Awassi lambs. Animal Feed Science and Technology 113, 61–69.
The effect of adding ruminally protected fat in fattening diets on nutrient intake, digestibility and growth performance of Awassi lambs.Crossref | GoogleScholarGoogle Scholar |

Hess BW, Moss GE, Rule DC (2008) A decade of developments in the area of fat supplementation research with beef cattle and sheep. Journal of Animal Science 86, E188–E204.
A decade of developments in the area of fat supplementation research with beef cattle and sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1c3lsFSjsg%3D%3D&md5=6b30631072c0361f4c17441be6d00f25CAS | 18156350PubMed |

Hristov AN, Ivan M, Rode LM, McAllister TA (2001) Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium- or high-concentrate barley-based diets. Journal of Animal Science 79, 515–524.

Jenkins TC (1993) Lipid metabolism in the rumen. Journal of Dairy Science 76, 3851–3863.
Lipid metabolism in the rumen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXhtlShtb4%3D&md5=6bee99b0979f3de6aa8abe46c8f42019CAS | 8132891PubMed |

Jouany JP (1996) Effect of rumen protozoa on nitrogen utilization by ruminants. The Journal of Nutrition 126, 1335S–1346S.

Kaneko JJ (1989) ‘Clinical biochemistry of domestic animals.’ 4th edn. (Academic Press Inc.: New York)

Leon AE, Olmos MC, Cruz E, Garcia R (1999) Accumulation of body fat in Cuban Pelibuey lambs according to age and feeding level. Archiva Zootechnica 48, 219–222.

Machmüller A, Ossowski DA, Kreuzer M (2000) Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Animal Feed Science and Technology 85, 41–60.
Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs.Crossref | GoogleScholarGoogle Scholar |

Manso T, Castro T, Mantecon AR, Jimeno V (2006) Effects of palm oil and calcium soaps of palm oil fatty acids in fattening diets on digestibility, performance and chemical body composition of lambs. Animal Feed Science and Technology 127, 175–186.
Effects of palm oil and calcium soaps of palm oil fatty acids in fattening diets on digestibility, performance and chemical body composition of lambs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XisF2it7o%3D&md5=a42c3722d950b324ea2ebf0cc435b3b1CAS |

Naik PK, Saijpaul S, Rani N (2007) Evaluation of rumen protected fat prepared by fusion method. Animal Nutrition and Feed Technology 7, 95–101.

Nigdi ME, Loerch SC, Fluharty FL, Palmiquist DL (1990) Effect of calcium soaps of long chain fatty acids on feedlot performance, carcass characteristics and ruminal metabolism of steers. Journal of Animal Science 68, 2555–2565.

NRC (2007) ‘Nutrient requirements of small ruminants.’ (National Research Council. US National Academy of Science: Washington, DC)

Perez JM, Bories G, Aumaitre A, Barrier-Guillet B, Delaveau A, Gueguen L, Labrier M, Sauvant D (2002) Consequences en elevage et pour le consommateur du remplacement des farines et des graisses animals. INRA Productions Animales 15, 87–96.

Robertson JB, Van Soest PJ (1981) ‘Detergent system of analysis and its application to human foods.’ (Cornell University: Ithaca, New York)

Sahoo A, Agarwal N, Kamra DN, Chaudhary LC, Pathak NN (1999) Influence of the level of molasses in deoiled rice bran based concentrate mixture on rumen fermentation pattern in crossbred calves. Animal Feed Science and Technology 80, 83–90.
Influence of the level of molasses in deoiled rice bran based concentrate mixture on rumen fermentation pattern in crossbred calves.Crossref | GoogleScholarGoogle Scholar |

Sahoo A, Singh B, Sharma OP (2011) Evaluation of feeding value of Eupatorium adenophorum in combination with mulberry leaves. Livestock Science 136, 175–183.
Evaluation of feeding value of Eupatorium adenophorum in combination with mulberry leaves.Crossref | GoogleScholarGoogle Scholar |

Salinas J, Ramírez RG, Domínguez MM, Reyes-Bernal N, Trinidad-Lárraga N, Montaño MF (2006) Effect of calcium soaps of tallow on growth performance and carcass characteristics of Pelibuey lambs. Small Ruminant Research 66, 135–139.
Effect of calcium soaps of tallow on growth performance and carcass characteristics of Pelibuey lambs.Crossref | GoogleScholarGoogle Scholar |

Santra A, Karim SA (2002) Influence of ciliate protozoa on biochemical changes and hydrolytic enzyme profile in the rumen ecosystem. Journal of Applied Microbiology 92, 801–811.
Influence of ciliate protozoa on biochemical changes and hydrolytic enzyme profile in the rumen ecosystem.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XksVSrsLw%3D&md5=61e1a649799f611cbee9da0a7bc6501aCAS | 11972682PubMed |

Santra A, Karim SA, Chaturvedi OH (2002) Effect of concentrate supplementation on nutrient intake and performance of lambs of two genotypes grazing a semiarid rangeland. Small Ruminant Research 44, 37–45.
Effect of concentrate supplementation on nutrient intake and performance of lambs of two genotypes grazing a semiarid rangeland.Crossref | GoogleScholarGoogle Scholar |

Schauff DJ, Clark JH (1992) Effects of feeding diets containing calcium salts of long chain fatty acids to lactating dairy cows. Journal of Dairy Science 75, 2990–3002.
Effects of feeding diets containing calcium salts of long chain fatty acids to lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXhtVSqtb4%3D&md5=2c0c7f1dc3ef83152806bcd00e1df832CAS | 1460131PubMed |

Sejian V, Maurya VP, Naqvi SMK (2010) Adaptability and growth of Malpura ewes subjected to thermal and nutritional stress. Tropical Animal Health and Production 42, 1763–1770.
Adaptability and growth of Malpura ewes subjected to thermal and nutritional stress.Crossref | GoogleScholarGoogle Scholar | 20571921PubMed |

Shipe WF, Senyk GF, Fountain KB (1980) Modified copper soap solvent extraction method for measuring free fatty acids in milk. Journal of Dairy Science 63, 193–198.
Modified copper soap solvent extraction method for measuring free fatty acids in milk.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3cXhs1ahtLg%3D&md5=73cbf304244963b3151d562da0681c6dCAS |

Singh VK, Karim SA (2003) Emerging trends in meat production from sheep and its export potential. In ‘Proceeding on meat production and export potential: impact of globalization and quality concepts’. (Eds SK Jindal, MK Agnihotri, V Raj Kumar, S Kumar, R Rana) pp. 51–59. (Central Institute for Research on Goats: Makhdoom, India)

Sklan D, Nagar L, Arieli A (1990) Effect of feeding different levels of fatty acids or calcium soaps of fatty acids on digestion and metabolizable energy in sheep. Animal Production 50, 93–98.
Effect of feeding different levels of fatty acids or calcium soaps of fatty acids on digestion and metabolizable energy in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXlsFWhsbk%3D&md5=590cc03827109b7f1abc5118a0e045bfCAS |

Trinder P (1969) Determination of blood glucose using an oxidase-peroxidase system with a non carcinogenic chromogen. Journal of Clinical Pathology 22, 158–161.
Determination of blood glucose using an oxidase-peroxidase system with a non carcinogenic chromogen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF1MXhtFaht7o%3D&md5=be42c22b6ee831492fced809353a3e6bCAS | 5776547PubMed |

Tripathi MK, Chaturvedi OH, Karim SA, Singh VK, Sisodiya SL (2007) Effect of different levels of concentrate allowances on rumen fluid pH, nutrient digestion, nitrogen retention and growth performance of weaner lambs. Small Ruminant Research 72, 178–186.
Effect of different levels of concentrate allowances on rumen fluid pH, nutrient digestion, nitrogen retention and growth performance of weaner lambs.Crossref | GoogleScholarGoogle Scholar |

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods of dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Symposium: carbohydrate methodology, metabolism and nutritional implications in dairy cattle. Journal of Dairy Science 74, 3583–3597.
Methods of dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Symposium: carbohydrate methodology, metabolism and nutritional implications in dairy cattle.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=1b837d0361716b7c813fff797a7701e0CAS | 1660498PubMed |

Weatherburn MW (1967) Phenol hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.
Phenol hypochlorite reaction for determination of ammonia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2sXksFSqtLY%3D&md5=bab272d2ffa933e2992350b291e5f3f0CAS |

Wybenga DR, Pilegg VJ, Dirstine PH, Giorgio JD (1970) Direct manual determination of serum total cholesterol with a single stable reagent. Clinical Chemistry 16, 980–984.

Zinn RA (1989) Influence of level and source of dietary fat on its comparative feeding value in finishing diets for steers: feedlot cattle growth and performance. Journal of Animal Science 67, 1029–1037.

Zinn RA, Gulati SK, Plascencia A, Salinas J (2000) Influence of ruminal biohydrogenation on the feeding value of fat in finishing diets for feedlot cattle. Journal of Animal Science 78, 1738–1746.