Evaluation of semen characteristics of the species Mazama americana in captivity
Luciana Diniz Rola A B , Eveline dos Santos Zanetti A and José Maurício Barbanti Duarte AA Deer Research and Conservation Centre (NUPECCE – Núcleo de Pesquisa e Conservação de Cervídeos), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Univ. Estadual Paulista (UNESP), Department of Animal Science, Jaboticabal, São Paulo, Brazil.
B Corresponding author. Email: lucianadinizr@gmail.com
Animal Production Science 53(5) 472-477 https://doi.org/10.1071/AN12247
Submitted: 20 July 2012 Accepted: 30 October 2012 Published: 22 February 2013
Abstract
In recent years the concept of genomic resource banks has grown as a way of maintaining the genetic variability of populations, while the quality of cryopreservation of the gametes determines the effectiveness of such banks. However, the absence of basic knowledge regarding the physiology of species and their semen characteristics hampers the establishment of reproductive biotechnologies. Thus, this paper aimed to determine certain physicochemical (volume, colour, appearance, pH and osmolarity) and microscopic characteristics (mass movement, motility, vigour, concentration, and sperm morphology and morphometry) of semen of the species Mazama americana. To achieve this, five males of the species were used, and three semen samples per buck (electroejaculation) were collected at intervals of 2 weeks. The volume, pH and osmolarity of the ejaculate were 0.39 ± 0.14 mL, 6.90 ± 0.74 and 297.74 ± 19.10 mOsm/kg, respectively, while the values obtained for mass movement, motility, vigour and concentration were 3.33 ± 0.82; 69.6 ± 8.92%; 3.53 ± 0.50, and 244.07 ± 98.65 × 107/mL, respectively. Regarding the colour of the ejaculate, five samples were classified as ivory, two as yellowish, two as whitish and six as white. Regarding appearance, seven samples were considered creamy and eight, milky. Morphology was analysed in a humid chamber under phase contrast microscopy and 73.50 ± 5.57% of cells presented normal morphology, 8.37 ± 3.15% presented major defects and 18.13 ± 6.46% presented minor defects. To determine sperm morphometry, an optical microscope (Leica DM 5000B) and the Leica Qwin image analyser program were used, resulting in 8.09 ± 0.40, 4.65 ± 0.30, 2.81 ± 0.44 and 30.25 ± 3.02 μm for length, largest width, smallest width and area, respectively.
Additional keywords: cervids, electroejaculation, genomic banks, red brocket deer.
References
Abreu CO, Martinez AC, Moraes W, Juvenal JC, Moreira N (2009) Características reprodutivas de veado-bororó-do-sul ou veado-mão-curta (Mazama nana). Pesquisa Veterinária Brasileira 29, 993–998.| Características reprodutivas de veado-bororó-do-sul ou veado-mão-curta (Mazama nana).Crossref | GoogleScholarGoogle Scholar |
Asher GW, Berg DK, Evans G (2000) Storage of semen and artificial insemination in deer. Animal Reproduction Science 62, 195–211.
| Storage of semen and artificial insemination in deer.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlt1Kgtr8%3D&md5=6994e73486d009e3f67543d24b22d34aCAS |
Barrozo LA, Toniolo GH, Duarte JMB, Pinho MP, Oliveira JA (2001) Padrão anual de variação da testosterona sérica, volume testicular e aspectos seminais de veados-catigueiros (Mazama gouazoubira) em cativeiro. Revista Brasileira de Reprodução Animal 25, 210–211.
Barth AD, Oko RJ (1989) ‘Abnormal morphology of bovine spermatozoa.’ (Iowa University Press: Ames)
Bielanski W, Kaczmarski F (1979) Morphology of spermatozoa in semen of stallions of normal fertility. Journal of Reproduction and Fertility 27, 39–45.
Blom E (1973) Ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermiogram. Nordisk Veterinaermedicin 25, 383–391.
Cheng FP, Wu JT, Chan JPW, Wang JS, Fung HP, Colenbrander B, Tung KC (2004) The effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Samba deer. Theriogenology 61, 1605–1616.
| The effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Samba deer.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhslWnurc%3D&md5=7682cb6987bc674c373bfdd4d23f59d8CAS |
Duarte JMB (2005) Coleta, conservação e multiplicação de recursos genéticos em animais silvestres: o exemplo dos cervídeos. Agrociencia 9, 541–544.
Duarte JMB, Garcia JM (1995) Reprodução assistida em Cervidae brasileiros. Revista Brasileira de Reprodução Animal 19, 111–121.
Favoretto SM, Zanetti ES, Duarte JMB (2012) Cryopreservation of red brocket deer semen (Mazama americana): comparison between three extenders. Journal of Zoo and Wildlife Medicine 43, 820–827.
| Cryopreservation of red brocket deer semen (Mazama americana): comparison between three extenders.Crossref | GoogleScholarGoogle Scholar |
Fernandes CE, Oliveira AR, Miranda PAB, Lopes SCP, Moraes SS, Morais MG, Abreu UGP, Landim-Alvarenga FC (2009) Alterações na morfologia espermática em touros de corte com e sem suplementação de zinco na mistura mineral. Ciência Animal Brasileira 10, 1074–1083.
Fernández-Santos MR, Martinez-Pastor F, Garcia-Macias V, Esteso MC, Soler AJ, De Paz P, Anel L, Garde JJ (2007) Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa. Theriogenology 67, 738–753.
| Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa.Crossref | GoogleScholarGoogle Scholar |
Freneau GE, Duarte JMB (2001) Biometria testicular e caracterÃsticas seminais de veado-catingueiro (Mazama gouazoubira) mantidos em cativeiro em Porto Nacional – TO. Ars Veterinária 17, 1–5.
Hafez ESE (2004) Avaliação do sêmen. In ‘Reprodução animal’. (Ed. ESE Hafez) pp. 369–379. (Editora Manole: São Paulo, Brazil)
Holt WV, Pickard AR (1999) Role of reproductive technologies and genetic resource banks in animal conservation. Reviews of Reproduction 4, 143–150.
| Role of reproductive technologies and genetic resource banks in animal conservation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXmsVClsr8%3D&md5=bb4193c02f7d7d60570849a815728083CAS |
IUCN (2011) IUCN Red List of Threatened Species. Version 2011.2. Available at http://www.iucnredlist.org [Verified 28 May 2012]
Martínez-Pastor F, Martínez F, García-Macías V, Esteso MC, Anel E, Fernández-Santos MR, Soler AJ, De Paz P, Garde J, Anel L (2006) A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality. Theriogenology 66, 1165–1172.
| A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality.Crossref | GoogleScholarGoogle Scholar |
Merino ML, Gonzales S, Leeuwenberg F, Rodrigues FHG, Pinder L, Tomas WN (1997) Veado-Campeiro (Ozotoceros bezoarticus). In ‘Biologia e conservação de cervídeos sul-americanos: Blastocerus, Ozotocerus e Mazama’. (Ed. JMB Duarte) pp. 42–58. (FUNEP: Jaboticabal, Brazil)
Papa FO, Alvarenga MA, Bicudo SD, Lopes MD, Ramires PRN (1986) Coloração espermática segundo Karras modificado pelo emprego do Barbatimão (Sthyphnodendrum barbatiman). In ‘Fifth Congresso de Biologia Celular, Rio de Janeiro, Brazil’.
Pereira RJG (2010) Male reproduction. In ‘Neotropical Cervidology’. (Eds JMB Duarte, S Gonzalez) pp. 39–50. (FUNEP/IUCN: Jaboticabal, Brazil)
Peroni EFC, Salviano MB, Suzuki MC, Christofolletti MD, Zanetti ES, Duarte JMB (2010) The surprising characteristic of the Amazonian Brown brocket deer (Mazama nemorivaga) semen: the red semen. In ‘Proceedings of the VIIth international deer biology congress’, (Ed. JA Smith-Flueck) pp. 56–57. (International Deer Biology Congress: Hilo-hilo)
Pinder L, Leeuwenberg F (1997) Veado-Catingueiro (Mazama gouazoubira, Fisher 1814), p.60-68. In ‘Biologia e Conservação de Cervídeos Sul-Americanos: Blastocerus, Ozotoceros e Mazama’. (Ed. JMB Duarte) pp. 60–68. (Funep: Jaboticabal, Brazil)
Varela DM, Trovati RG, Guzmán KR, Rossi RV, Duarte JMB (2010) Red brocket deer Mazama Americana (Erxleben 1777). In ‘Neotropical Cervidology’. (Eds JMB Duarte, S Gonzalez) pp. 151–159. (FUNEP/IUCN: Jaboticabal, Brazil)
Versiani NF, Pereira RG, Duarte JMB (2009) Annual variations in fecal androgen metabolites and antler cycle of captive red brocket bucks (Mazama americana) in southeast Brazil. European Journal of Wildlife Research 55, 535–538.
| Annual variations in fecal androgen metabolites and antler cycle of captive red brocket bucks (Mazama americana) in southeast Brazil.Crossref | GoogleScholarGoogle Scholar |
Wildt DE (1996) Male reproduction: assessment, management, and control of fertility. In ‘Wild mammals in captivity’. (Eds DG Kleiman, ME Allen, KV Thompson, S Lumpkin) pp. 129–150. (University of Chicago Press: Chicago)
Zanetti ES, Duarte JMB, Polegato BF, Garcia JM, Canola JC (2010) Assisted reproductive technology. In ‘Neotropical Cervidology’. (Eds JMB Duarte, S Gonzalez) pp. 255–270. (FUNEP/IUCN: Jaboticabal, Brazil)
