Use of laparoscopic intrauterine insemination associated with a simplified superovulation treatment for in vivo embryo production in sheep: a preliminary report
F. Forcada A , L. Sánchez-Prieto A , A. Casao B , I. Palacín A , J. A. Cebrián-Pérez B , T. Muiño-Blanco B and J. A. Abecia A C
+ Author Affiliations
- Author Affiliations
A Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain.
B Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain.
C Corresponding author. Email: alf@unizar.es
Animal Production Science 52(12) 1111-1116 https://doi.org/10.1071/AN12129
Submitted: 12 April 2012 Accepted: 29 June 2012 Published: 4 September 2012
Abstract
This study evaluated the reproductive performance of sheep that were subjected to superovulatory treatment and intrauterine insemination with two different doses of semen. The main objective was to determine whether intrauterine insemination is associated with higher rates of embryo recovery than existing procedures. In the first experiment 30 Ojalada de Soria ewes were used at the end of their productive life and nine younger ewes of the same breed were used in Experiment 2. Synchronisation of oestrus was conducted with intravaginal sponges and the superovulation treatment consisted of an intramuscular injection of 210 IU pFSH and 500 IU eCG administered 24 h before sponge removal. After 48 h of sponge withdrawal, ewes were inseminated by laparoscopy. They were divided into two groups: low dose group (LD, 25 × 106 sperm; n = 14 Expt 1 and n = 5 Expt 2) and high dose group (HD, 100 × 106 sperm; n = 14 Expt 1 and n = 4 Expt 2). Embryos were recovered 7 days after the onset of oestrus by laparotomy and uterine flushing. Two blood samples were collected in order to analyse cortisol, at sponge insertion and the day the embryos were recovered. The same protocol was repeated twice in Experiment 1, with an interval of 2 months between two consecutive treatments. Results show a significant effect of the number of recovery on ovulation rate (21.7 ± 2.2 v. 11.8 ± 1.1 corpora lutea for first and second recoveries, respectively), and number of structures recovered (11.4 ± 1.6 v. 5.2 ± 1.1, P < 0.01). Differences of embryo performance in the second recovery compared with the first one were more evident in the LD group compared with the HD group. In Experiment 2, results reflected a significant superiority of the HD group compared with the LD group, especially rate of fertilisation, which was 25% higher. Sheep producing non-fertilised embryos had higher cortisol levels at the time of flushing than those with embryos (P < 0.01). It is concluded that the use of simplified and consecutive superovulatory treatments associated with intrauterine insemination is a potential means of obtaining embryos, although the response seems to depend on donor sheep age. The use of a higher number of sperm cells per millilitre results in better outcomes in terms of embryos produced.
References
Armstrong DT, Evans G (1984) Intrauterine insemination enhances fertility of frozen semen in superovulated ewes. Journal of Reproduction and Fertility 71, 89–94.| Intrauterine insemination enhances fertility of frozen semen in superovulated ewes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL2c3hs1Wjtg%3D%3D&md5=3b458f269717a73f187383750faf1e2eCAS |
Azawi OI, Al-Mola MKMA (2010) Effect of season and mating system in Awassi ewes superovulated with FSH on fertilization rate and embryo recovery. Iraqi Journal of Veterinary Sciences 24, 75–79.
Bari F, Khalid M, Haresign W, Murray A, Merrell B (2000) Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep. Theriogenology 53, 727–742.
| Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c7pvFaitg%3D%3D&md5=9fe08a81f9a4e6c1b7c08d232a80ec34CAS |
Bettencourt EM, Bettencourt CM, Chagas e Silva J, Ferreira P, Pereira-Matos C, Romao RJ, Rocha A (2009) Fertility rates following the transfer of ovine embryos cryopreserved using three protocols. Small Ruminant Research 82, 112–116.
| Fertility rates following the transfer of ovine embryos cryopreserved using three protocols.Crossref | GoogleScholarGoogle Scholar |
Breen K, Billings HJ, Wagenmaker ER, Wessinger EW, Karsch FR (2005) Endocrine basis for disruptive effects of cortisol on preovulatory events. Endocrinology 146, 2107–2115.
| Endocrine basis for disruptive effects of cortisol on preovulatory events.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXis12jsrw%3D&md5=aa89d5df5b5001008915643b8e45b4feCAS |
Chacón G, Garcia-Belenguer S, Ilguera JC, Palacio J (2004) Validation of an EIA technique for the determination of cortisol. Spanish Journal of Agricultural Research 2, 45–51.
Cueto MI, Gibbons AE, Pereyra-Bonnet F, Silvestre P, Gonzalez-Bulnes A (2011) Effects of season and superovulatory treatment on embryo yields in fine-wool Merinos maintained under field conditions. Reproduction in Domestic Animals 46, 770–775.
| Effects of season and superovulatory treatment on embryo yields in fine-wool Merinos maintained under field conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlemtrzO&md5=0d08cf0b6cabf5bfb9db99aa5285af6aCAS |
de Graaf SP, Beilby K, O’Brien JK, Osborn D, Downing JA, Maxwell WMC, Evans G (2007) Embryo production from superovulated sheep inseminated with sex-sorted spermatozoa. Theriogenology 67, 550–555.
| Embryo production from superovulated sheep inseminated with sex-sorted spermatozoa.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2s%2Fht1Cruw%3D%3D&md5=46d1affbb0586450b96812d244fbaecaCAS |
Dobson H, Ghuman S, Prabhakar S, Smith R (2003) A conceptual model of the influence of stress on female reproduction. Reproduction (Cambridge, England) 125, 151–163.
| A conceptual model of the influence of stress on female reproduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXisVKltrg%3D&md5=c5637fd3efda1121fca5718eb027d444CAS |
Ehling C, Wirth P, Schindler L, Hadeler KG, Dopke HH, Lemme E, Herrman D, Niemann H (2003) Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes. Theriogenology 60, 777–787.
| Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes.Crossref | GoogleScholarGoogle Scholar |
Forcada F, Abecia JA, Sierra I (1992) Seasonal changes in oestrus activity and ovulation rate in Rasa Aragonesa ewes maintained at two different body condition levels. Small Ruminant Research 8, 313–324.
| Seasonal changes in oestrus activity and ovulation rate in Rasa Aragonesa ewes maintained at two different body condition levels.Crossref | GoogleScholarGoogle Scholar |
Forcada F, Abecia JA, Lozano JM, Zúñiga O (2000) Repeated superovulation of high-prolificacy Rasa Aragonesa ewes before culling as an inexpensive way to obtain high-quality embryos. Livestock Production Science 66, 263–269.
| Repeated superovulation of high-prolificacy Rasa Aragonesa ewes before culling as an inexpensive way to obtain high-quality embryos.Crossref | GoogleScholarGoogle Scholar |
Forcada F, Abecia JA, Cebrián-Pérez JA, Muiño-Blanco T, Valares JA, Palacín I, Casao A (2006) The effect of melatonin implants during the seasonal anestrus on embryo production after superovulation in aged high-prolifacy Rasa Aragonesa ewes. Theriogenology 65, 356–365.
| The effect of melatonin implants during the seasonal anestrus on embryo production after superovulation in aged high-prolifacy Rasa Aragonesa ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlCqtb%2FK&md5=af2c2dc772b40b1ff4e1d5fea1094732CAS |
Forcada F, Ait-Am.-Meziane M, Abecia JA, Maurel MC, Cebrián-Pérez JA, Muiño-Blanco T, Asenjo B, Vázquez MI, Casao A (2011) Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep. Theriogenology 75, 769–776.
| Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhslKqt7Y%3D&md5=b979a27ddd504e396e0eb0cdfe477aebCAS |
Fukui Y, Roberts EM (1977) Sperm transport after non-surgical intrauterine inseminations with frozen semen in ewes treated with prostaglandin F2α. Journal of Reproduction and Fertility 51, 141–143.
| Sperm transport after non-surgical intrauterine inseminations with frozen semen in ewes treated with prostaglandin F2α.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXosFA%3D&md5=04ac14869fb7a08f85dd72c555275866CAS |
Killen ID, Caffery GJ (1982) Uterine insemination of ewes with the aid of a laparoscope. Australian Veterinary Journal 59, 95
| Uterine insemination of ewes with the aid of a laparoscope.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL3s7is1egsQ%3D%3D&md5=db7476a5c4219444e71a6e6e9467fcbeCAS |
Ledda S, Naitana S, Cappai S, Branca A, Loi P, Forcada F, Abecia JA, Zarazaga L (1992) Efecto del tratamiento PMSG + FSHp sobre la superovulación en ovejas Sarda y Rasa Aragonesa. In ‘Proceedings of the VI Jornadas Internacionales de Reproducción Animal e Inseminación Artificial’. pp. 298–303. (Asociación Española de Especialistas en Reproducción Animal: Salamanca, Spain)
Leoni G, Bogliolo L, Pintus P, Ledda S, Naitana S (2001) Sheep embryos derived from FSH/eCG treatment haver a lower in vitro viability after vitrification than those derived from FSH treatment. Reproduction, Nutrition, Development 41, 239–246.
| Sheep embryos derived from FSH/eCG treatment haver a lower in vitro viability after vitrification than those derived from FSH treatment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnvVantb0%3D&md5=df6565b31a2d124c64b8de6f6fcce725CAS |
Lindner GM, Wright RW (1983) Bovine embryo morphology and evaluation. Theriogenology 20, 407–416.
| Bovine embryo morphology and evaluation.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD283pvVKgsg%3D%3D&md5=4321d18c5aff0640942132fbf576f0baCAS |
Lymberopoulos AG, Amiridis GS, Kuhholzer B, Besenfelder U, Christidoulou V, Vainas E, Brem G (2001) Fertilization and embryo recovery rates in superovulated Chios ewes after laparoscopic intrauterine insemination. Theriogenology 55, 1855–1862.
| Fertilization and embryo recovery rates in superovulated Chios ewes after laparoscopic intrauterine insemination.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3MzltV2qsA%3D%3D&md5=f4a7f68fe60399cdf475b296f5eedb07CAS |
McKelvey WAC, Robinson JJ, Aitken RP (1985) The evaluation of a laparoscopic insemination technique in ewes. Theriogenology 24, 519–535.
| The evaluation of a laparoscopic insemination technique in ewes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD283pvVyktg%3D%3D&md5=39b9af894cbd1eaa9e555925e03dbbb6CAS |
McKusick BC, Thomas DL, Gottfredson RG, Zelinsky RD, Berger YM (1998) A comparison of transcervical and laparoscopic intrauterine artificial insemination techniques on reproductive performance of ewes. In ‘Proceedings 46th annual spooner sheep day’. pp. 32–39. (Cooperative Extension of the University of Wisconsin-Extension: Madison, WI)
Naqvi SMK, Gulyani R (1999) Ovarian response and embryo recovery to different superovulatory regimens in Rambouillet ewes under semiarid conditions. Small Ruminant Research 34, 127–131.
| Ovarian response and embryo recovery to different superovulatory regimens in Rambouillet ewes under semiarid conditions.Crossref | GoogleScholarGoogle Scholar |
Okada M, Ishida N, Ogiso T, Itagaki R, Ishikawa D, Fukui Y (1999) Effect of dosage of equine chorionic gonadotropin combined with a single injection of porcine follicle-stimulating hormone for superovulation treatment in ewes. The Journal of Reproduction and Development 45, 307–313.
| Effect of dosage of equine chorionic gonadotropin combined with a single injection of porcine follicle-stimulating hormone for superovulation treatment in ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXivFeltw%3D%3D&md5=d5dc8a264c7c4a147de6158e3ea6e8a0CAS |
Perkins NR, Hill JR, Pedrana RG (1996) Laparoscopic insemination of frozen-thawed semen into one or both uterine horns without regard to ovulation site in synchronized merino ewes. Theriogenology 46, 541–545.
| Laparoscopic insemination of frozen-thawed semen into one or both uterine horns without regard to ovulation site in synchronized merino ewes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zgtVKktA%3D%3D&md5=1d9de01af763c25bc27c12032cd407a7CAS |
Rexroad CE, Jr, Brinster RL, Hammer RE (1984) Fertility of ova after natural or intrauterine insemina tion of superovulated ewes at natural or progestin sponge regulated estrus. In ‘Proceedings Northeast Region American Society of Animal Science’. p. 139.
Riesenberg S, Meinecke-Tillmann S, Meinecke B (2001) Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats. Small Ruminant Research 40, 83–93.
| Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats.Crossref | GoogleScholarGoogle Scholar |
Ryan JP, Hunton JR, Maxwell WMC (1991) Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone. Reproduction, Fertility and Development 3, 551–580.
| Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38Xlt1Cku70%3D&md5=e0a068286ecc6ebd54750834740b99b9CAS |
Schiewe MC, Fitz TA, Brown JL, Stuart LD, Wildt DE (1991) Relationship of estrus synchronization method, circulating hormones, luteinizing-hormone and prostaglandin F2 alpha receptors and luteal progesterone concentration to premature luteal regression in superovulated sheep. Journal of Reproduction and Fertility 93, 19–30.
| Relationship of estrus synchronization method, circulating hormones, luteinizing-hormone and prostaglandin F2 alpha receptors and luteal progesterone concentration to premature luteal regression in superovulated sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmt1Cls70%3D&md5=d2a7c2790287623414afff83a0a89b99CAS |
Simonetti L, Forcada F, Rivera OE, Carou N, Alberio RH, Abecia JA, Palacín I (2008) Simplified superovulatory treatments in Corriedale ewes. Animal Reproduction Science 104, 227–237.
| Simplified superovulatory treatments in Corriedale ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVOmsLk%3D&md5=9b18c8b82f44c4ef7206ac56ce2c2e74CAS |
Tervit HR, Goold PG, McKenzie RD (1984) Embryo transfer in Angora and Saanen goats. Theriogenology 21, 269
| Embryo transfer in Angora and Saanen goats.Crossref | GoogleScholarGoogle Scholar |
Watanabe H, Miyamoto A, Okada M, Ishida N, Kimura H, Fukui Y (1998) A simple superovulation method of a single injection of FSH combined with eCG for superovulation of Suffolk ewes during the breeding season I. Effects of different treatments on the endocrine profiles. The Journal of Reproduction and Development 44, 169–176.
| A simple superovulation method of a single injection of FSH combined with eCG for superovulation of Suffolk ewes during the breeding season I. Effects of different treatments on the endocrine profiles.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXlsF2rt7o%3D&md5=0c37007102e20bf7e9b44a4dde3fcebdCAS |
Winterberger-Torres S, Sevellec C (1987) ‘Atlas of the early development of sheep embryos.’ (INRA: Paris)
