Preliminary study of the changes in rumen bacterial populations from cattle intoxicated with young oak (Quercus pyrenaica) leaves
A. Belenguer A D , G. Hervás A , D. R. Yáñez-Ruiz B , P. G. Toral A , C. Ezquerro C and P. Frutos AA Instituto de Ganadería de Montaña, CSIC – Universidad de León, Finca Marzanas s/n, 24346 Grulleros, León, Spain.
B Unidad de Nutrición Animal, Estación Experimental del Zaidín, CSIC, Camino del jueves s/n, 18100 Armilla, Granada, Spain.
C Instituto de Biología Molecular, Genómica y Proteómica, Universidad de León, Campus de Vegazana, 24071 León, Spain.
D Corresponding author. Email: a.belenguer@eae.csic.es
Animal Production Science 50(3) 228-234 https://doi.org/10.1071/AN09154
Submitted: 13 November 2009 Accepted: 17 March 2010 Published: 8 April 2010
Abstract
Intoxication of grazing cattle occurs repeatedly when they consume large amounts of young oak leaves (OL), which are rich in hydrolysable tannins (HT), due to a shortage of other feed resources. The HT are antimicrobial, although some rumen bacteria can resist or degrade them into potentially toxic or harmless metabolites. To study the effect of the administration of HT-rich OL (Quercus pyrenaica) after a severe feed restriction on the rumen bacterial community and monitor the variations in some bacterial groups that are potentially able to resist or metabolise tannins, three ruminally cannulated bulls were initially fed grass hay and then subjected to a severe 8-day feed restriction period, before receiving OL for 6 days. Then, the animals were again offered grass hay for 12 more days. Rumen contents were sampled throughout the experiment. Quantitative real-time polymerase chain reaction and terminal restriction fragment length polymorphism were used to monitor the bacterial dynamics. Animal 1 was not intoxicated and showed lower relative abundances of Streptococcus bovis initially and after the OL administration than Animals 2 and 3, which showed acute signs of intoxication. The genus Prevotella increased its abundance with the OL administration, whereas Selenomonas ruminantium was reduced. The bacterial terminal restriction fragment length polymorphism profile of Animal 1 clustered initially separately from Animals 2 and 3 and was less affected by the feed restriction period. These results showed that the effect of the consumption of HT-rich OL after a severe feed restriction is highly variable in cattle and might rely on the individual composition of the microbiota colonising the rumen.
Additional keywords: hydrolysable tannins, real-time polymerase chain reaction, rumen bacteria, terminal restriction fragment length polymorphism.
Acknowledgements
This work was supported by the Ministry of Education and Science (project AGL2004-06076-C02-01). P. G. Toral gratefully acknowledges receipt of a predoctoral grant from the Spanish National Research Council (CSIC, I3P Program), and the authors wish to thank Drs S. López and B. Rabanal for kindly providing the DNA from pure cultures and technical assistance, respectively, and Dr C. Walker for revision of the manuscript.
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