Expression patterns of two carbonic anhydrase genes, Na+/K+-ATPase and V-type H+-ATPase, in the freshwater crayfish, Cherax quadricarinatus, exposed to low pH and high pH
Muhammad Yousuf Ali A , Ana Pavasovic B , Peter B. Mather A and Peter J. Prentis A C
+ Author Affiliations
- Author Affiliations
A School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia.
B School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia.
C Corresponding author. Email: p.prentis@qut.edu.au
Australian Journal of Zoology 65(1) 50-59 https://doi.org/10.1071/ZO16048
Submitted: 19 July 2016 Accepted: 10 May 2017 Published: 8 June 2017
Abstract
Carbonic anhydrase (CA), Na+/K+-ATPase (NKA) and Vacuolar-type H+-ATPase (HAT) play vital roles in osmoregulation and pH balance in decapod crustaceans. As variable pH levels have a significant impact on the physiology of crustaceans, it is crucial to understand the mechanisms by which an animal maintains its internal pH. We examined expression patterns of cytoplasmic (CAc) and membrane-associated form (CAg) of CA, NKA α subunit and HAT subunit a in gills of freshwater crayfish, Cherax quadricarinatus, at three pH levels – 6.2, 7.2 (control) and 8.2 – over 24 h. Expression levels of CAc were significantly increased at low pH and decreased at high pH conditions 24 h after transfer. Expression increased at low pH after 12 h, and reached its maximum level by 24 h. CAg showed a significant increase in expression at 6 h after transfer at low pH. Expression of NKA significantly increased at 6 h after transfer to pH 6.2 and remained elevated for up to 24 h. Expression for HAT and NKA showed similar patterns, where expression significantly increased 6 h after transfer to low pH and remained significantly elevated throughout the experiment. Overall, CAc, CAg, NKA and HAT gene expression is induced at low pH conditions in freshwater crayfish.
Additional keywords: acid–base, expression, gills, osmoregulation, pH balance, redclaw.
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