Effects of dimethyl sulfoxide (DMSO) on DNA methylation and histone modification in parthenogenetically activated porcine embryos
Hui Cheng A # , Yu Han A # , Jian Zhang A , Sheng Zhang B , Yanhui Zhai A , Xinglan An B , Qi Li B , Jiahui Duan A , Xueming Zhang A , Ziyi Li B , Bo Tang
A * and Haiqing Shen A *
+ Author Affiliations
- Author Affiliations
A College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
B Academy of Translational Medicine, First Hospital, Jilin University, Changchun, Jilin 130021, China.
* Correspondence to: tang_bo@jlu.edu.cn, shenhq@jlu.edu.cn
# These authors contributed equally to this paper
Handling Editor: Ye Yuan
Reproduction, Fertility and Development 34(8) 598-607 https://doi.org/10.1071/RD21083
Published online: 11 April 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Epigenetic mechanisms play an important role in oogenesis and early embryo development in mammals. Dimethyl sulfoxide (DMSO) is frequently used as a solvent in biological studies and as a vehicle for drug therapy. Recent studies suggest that DMSO detrimentally affects porcine embryonic development, yet the mechanism of the process in parthenogenetically activated porcine embryos has not been reported. In this study, we found that treatment of embryos with 1.5% DMSO significantly decreased the cleavage and blastocyst rates, total cell number of blastocysts and the anti-apoptotic gene BCL-2 transcription level; however, the percentage of apoptotic cells and the expression levels of the pro-apoptotic gene BAX were not changed. Treatment with DMSO significantly decreased the expression levels of DNMT1, DNMT3a, DNMT3b, TET1, TET2, TET3, KMT2C, MLL2 and SETD3 in most of the stages of embryonic development and increased 5-mC signals, while the staining intensity for 5-hmC had no change in porcine preimplantation embryos from 2-cell to the blastocyst stages. Meanwhile, DMSO decreased the level of H3K4me3 during the development of parthenogenetically activated porcine embryos. After treatment with DMSO, expression levels of the pluripotency-related genes POU5F1 and NANOG decreased significantly (P < 0.01), whereas the imprinted gene H19 did not change (P > 0.05). In conclusion, these results suggest that DMSO can affect genome-wide DNA methylation and histone modification by regulating the expression of epigenetic modification enzymes, and DMSO also influences the expression level of pluripotent genes. These dysregulations lead to defects in embryonic development.
Keywords: DMSO, DNA methylation, embryonic development, gene, histone modification, parthenogenetically activated, porcine, preimplantation embryos.
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