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Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
REVIEW

Extracellular vesicles in the male reproductive tract of the softshell turtle

Qiusheng Chen A C and William V. Holt https://orcid.org/0000-0002-9039-8651 B
+ Author Affiliations
- Author Affiliations

A MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China.

B Academic Unit of Reproductive and Developmental Medicine, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.

C Corresponding author. Email: chenqsh305@njau.edu.cn

Reproduction, Fertility and Development 33(9) 519-529 https://doi.org/10.1071/RD20214
Submitted: 19 August 2020  Accepted: 28 January 2021   Published: 15 March 2021

Abstract

Extracellular vesicles (EVs) are a heterogeneous group of cell-derived membranous structures comprising exosomes and microvesicles that originate from the endosomal system or are shed from the plasma membrane respectively. As mediators of cell communication, EVs are present in biological fluids and are involved in many physiological and pathological processes. The role of EVs has been extensively investigated in the mammalian male reproductive tract, but the characteristics and identification of EVs in reptiles are still largely unknown. In this review we focus our attention on EVs and their distribution in the male reproductive tract of the Chinese softshell turtle Pelodiscus sinensis, mainly discussing the potential roles of EVs in intercellular communication during different phases of the reproductive process. In softshell turtles, Sertoli–germ cell communication via multivesicular bodies can serve as a source of EVs during spermatogenesis, and these EVs interact with epithelia of the ductuli efferentes and the principal cells of the epididymal epithelium. These EVs are involved in sperm maturation, transport and storage. EVs are also shed by telocytes, which contact and exchange information with other, as well as distant interstitial cells. Overall, EVs play an indispensable role in the normal reproductive function of P. sinensis and can be used as an excellent biomarker for understanding male fertility.

Graphical Abstract Image

Keywords: Chinese softshell turtle, extracellular vesicles (EVs), male reproductive tract, Pelodiscus sinensis.


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