Type I interferon receptors and interferon-τ-stimulated genes in peripheral blood mononuclear cells and polymorphonuclear leucocytes during early pregnancy in beef heifers
G. D. Melo
A , L. M. F. Pinto A , C. C. Rocha A , I. G. Motta A , L. A. Silva B , J. C. da Silveira B , A. M. Gonella-Diaza C , M. Binelli D and G. Pugliesi A E
+ Author Affiliations
- Author Affiliations
A Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, Avenida Duque de Caxias Norte, 225, Campus Fernando Costa, University of São Paulo, 13635-900, Pirassununga, SP, Brazil.
B Department of Veterinary Medicine, School of Animal Science and Food Engineering, Avenida Duque de Caxias Norte, 225, Campus Fernando Costa, University of São Paulo, 13635-900, Pirassununga, SP, Brazil.
C North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, 4932 Highway 162, Marianna, FL 32446, USA.
D Department of Animal Sciences, University of Florida, 2250 Shealy Drive, Gainesville, FL 32608, USA.
E Corresponding author. Email: gpugliesi@usp.br
Reproduction, Fertility and Development 32(11) 953-966 https://doi.org/10.1071/RD19430
Submitted: 27 November 2019 Accepted: 12 February 2020 Published: 10 July 2020
Abstract
This study characterised the expression of interferon (IFN)-τ-stimulated genes (ISGs) and Type I IFN receptors in circulating polymorphonuclear cells (PMNs) of beef heifers and compared it with expression in peripheral blood mononuclear cells (PBMCs) up to Day 20 of gestation. Nelore heifers (n = 26) were subjected to fixed-time AI (FTAI) on Day 0. PMNs and PBMCs were isolated on Days 0, 10, 14, 16, 18 and 20 after FTAI. The abundance of target transcripts (ubiquitin-like protein (ISG15), 2′-5′-oligoadenylate synthetase 1 (OAS1), myxovirus resistance 1 (MX1), myxovirus resistance 2 (MX2), IFN receptor I (IFNAR1) and IFN receptor 2 (IFNAR2)) was determined using real-time quantitative polymerase chain reaction and compared between pregnant (n = 8) and non-pregnant (n = 9) females. In both PBMCs and PMNs, ISG15 and OAS1 expression was greater in pregnant than non-pregnant heifers on Days 18 and 20. There were no significant differences in the expression of ISGs between PBMCs and PMNs. A time effect on expression was found for IFNAR1 in PBMCs and IFNAR2 in PMNs, with decreased expression of both genes on Days 18 and 20. When the expression of these genes was compared between cell types only in pregnant heifers, IFNAR2 expression in PMNs had an earlier decrease when compared to its expression in PBMCs, starting from Day 18. In conclusion, PMNs do not respond earlier to the conceptus stimulus, and ISG15 and OAS1 expression in both PMNs and PBMCs can be used as a suitable marker for pregnancy diagnosis on Days 18 and 20. In addition, gestational status did not affect IFNAR1 and IFNAR2 expression, but IFNAR2 showed a distinct response between PMNs and PBMCs of pregnant heifers.
Additional keyword: pregnancy diagnosis.
References
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