Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilisation
A. S. Alcântara-Neto A , M. Fernandez-Rufete B , E. Corbin A , G. Tsikis A , R. Uzbekov C D , A. S. Garanina C E , P. Coy B , C. Almiñana A F * and P. Mermillod
A G *
+ Author Affiliations
- Author Affiliations
A Unité Mixte de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique (INRA), 37380 Nouzilly, France.
B Department of Physiology, Faculty of Veterinary, University of Murcia, Murcia, IMIB-Arixaca, Spain.
C Laboratoire Biologie Cellulaire et Microscopie Electronique, Faculté de Médecine, Université François Rabelais, 37000 Tours, France.
D Faculty of Bioengineering and Bioinformatics, Moscow State University, 119992, Leninskye gory 73, Moscow, Russian Federation.
E Present address: National University of Science and Technology ‘MISiS’, 119049, Moscow, Russian Federation.
F Present address: University of Zurich, Genetics and Functional Genomics Group, Clinic of Reproductive Medicine, Department of Farm Animals, VetSuisse Faculty, Zurich, Switzerland.
G Corresponding author: Email: pascal.mermillod@inra.fr
Reproduction, Fertility and Development 32(4) 409-418 https://doi.org/10.1071/RD19058
Submitted: 11 February 2019 Accepted: 18 July 2019 Published: 28 November 2019
Abstract
High polyspermy is one of the major limitations of porcine in vitro fertilisation (IVF). The addition of oviductal fluid (OF) during IVF reduces polyspermy without decreasing the fertilisation rate. Because extracellular vesicles (EVs) have been described as important OF components, the aim of this study was to evaluate the effect of porcine oviductal EVs (poEVs) on IVF efficiency compared with porcine OF (fresh and lyophilised). OF was collected from abattoir oviducts by phosphate-buffered saline flush, and poEVs were isolated by serial ultracentrifugation. Four IVF treatments were conducted: poEVs (0.2 mg mL–1), OF (10%), lyophilized and reconstituted pure OF (LOF; 1%) and IVF without supplementation (control). Penetration, monospermy and IVF efficiency were evaluated. Transmission electron microscopy showed an EVs population primarily composed of exosomes (83%; 30–150 nm). Supplementation with poEVs during IVF increased monospermy compared with control (44% vs 17%) while maintaining an acceptable penetration rate (61% vs 78% respectively) in a similar way to OF and LOF. Western blotting revealed poEVs proteins involved in early reproductive events, including zona pellucida hardening. In conclusion, our finding show that poEVs are key components of porcine OF and may play roles in porcine fertilisation and polyspermy regulation, suggesting that supplementation with poEVs is a reliable strategy to decrease porcine polyspermy and improve in vitro embryo production outcomes.
Additional keywords: fallopian tube, oocyte, spermatozoa, zona pellucida.
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