Effects of ovary storage temperature and embryo vitrification on somatic cell nuclear transfer outcomes in goats
Mehdi Hajian A C , Farnoosh Jafarpour A , Sayed Morteza Aghamiri B , Shiva Rouhollahi Varnosfaderani A and Mohammad Hossein Nasr Esfahani
A C
+ Author Affiliations
- Author Affiliations
A Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Academic Center for Education, Culture and Research, Salman Street, Royan Street, 81593-58686, Isfahan, Iran.
B Department of Clinical Studies, School of Veterinary Medicine, Shahid Bahonar University of Kerman, 22th Bahman Street, 76169-14111, Kerman, Iran.
C Corresponding authors. Email: mh.nasr-esfahani@royaninstitute.org; Mehdihajian2002@gmail.com
Reproduction, Fertility and Development 32(4) 419-424 https://doi.org/10.1071/RD18529
Submitted: 8 October 2018 Accepted: 19 July 2019 Published: 10 December 2019
Abstract
Improving the genetic potential of farm animals is one of the primary aims in the field of assisted reproduction. In this regard, somatic cell nuclear transfer (SCNT) can be used to produce a large number of embryos from genetically elite animals. The aims of the present study were to assess the effects of: (1) ovary storage conditions on preimplantation development of recovered oocytes and the freezability of the derived blastocysts; and (2) vitrification of goat SCNT-derived blastocysts on postimplantation development. Goat oocytes were recovered from ovaries and stored under warm (25°C-27°C) or cold (11°C-12°C) conditions before being used to produce SCNT embryos. There were no differences in oocytes recovered from ovaries kept under cold versus warm storage conditions in terms of cleavage (mean (± s.d.) 95.68 ± 1.67% vs 95.91 ± 2.93% respectively) and blastocyst formation (10.69 ± 1.17% vs 10.94 ± 0.9% respectively) rates. The re-expansion rate of vitrified blastocysts was significantly lower for cold- than warm-stored ovaries (66.3 ± 8.7% vs 90 ± 11% respectively). To assess the effects of vitrification on postimplantation development, blastocysts from cold-stored ovaries only were transferred from fresh and vitrified–warmed groups. The pregnancy rate was comparable between the fresh and vitrified–warmed groups (41.65% and 45.45% respectively). In addition, established pregnancy in Day 28-38 and full-term pregnancy rates were similar between the two groups. In conclusion, this study shows similar in vitro preimplantation developmental potential of warm- and cold-stored ovaries. This study introduces the vitrification technique as an appropriate approach to preserve embryos produced by SCNT for transfer to recipient goats at a suitable time.
Additional keywords: blastocysts, somatic cell nuclear transfer-derived embryos.
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