Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus–oocyte complexes and blastocysts
Eduardo M. Razza A G , Mateus J. Sudano B , Patricia K. Fontes C , Fernanda F. Franchi C , Katia Roberta A. Belaz D , Priscila H. Santos C , Anthony C. S. Castilho E F , Daniele F. O. Rocha D , Marcos N. Eberlin D , Mariana F. Machado C and Marcelo F. G. Nogueira C F
+ Author Affiliations
- Author Affiliations
A Nove de Julho University, Rua Nicolau Assis, 15, 17011102, Bauru, São Paulo, Brazil.
B School of Veterinary Medicine, Federal University of Pampa, BR 472 Km 592, Caixa Postal 118, 97508000, Uruguaiana, Rio Grande do Sul, Brazil.
C Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Distrito de Rubião Junior s/n, 18618970, Botucatu, São Paulo, Brazil.
D ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas – UNICAMP, Rua Sérgio Buarque de Holanda s/n, 13083859, Campinas, São Paulo, Brazil.
E University of Western São Paulo, Rodovia Raposo Tavares, km 572 – Bairro Limoeiro, 19067175, Presidente Prudente, São Paulo, Brazil.
F Department of Biological Sciences, Faculty of Sciences and Letters, University of São Paulo State, Avenuenida Dom Antonio, 2100, 19806900, Assis, São Paulo, Brazil.
G Corresponding author. Email: eduardorazza@gmail.com
Reproduction, Fertility and Development 30(10) 1314-1328 https://doi.org/10.1071/RD17335
Submitted: 24 June 2017 Accepted: 15 March 2018 Published: 23 April 2018
Abstract
Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus–oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocysts and on the transcriptional profiling of cumulus cells and blastocysts in a high-throughput platform. Embryonic development rates to the morula (mean 56.1%) or blastocyst (mean 26.3%) stages were unaffected by treatment. Lipid content was not affected after Pre-IVM, but was increased after IVM in treated oocytes. Conversely, the lipid content was reduced in Pre-IVM blastocysts. Pre-IVM COCs generated blastocysts containing blastomeres with more unsaturated lipids in their membranes. Pre-IVM also altered the relative abundance of 31 gene transcripts after 2 h and 16 transcripts after 24 h in cumulus cells, while seven transcripts were altered in blastocysts. Our results suggest that the Pre-IVM treatment affected the lipid composition and transcriptional profiles of COCs and blastocysts. Therefore, Pre-IVM with FSK and IBMX could be used either to prevent spontaneous meiotic resumption during IVM or to modulate lipid composition in the membrane and cytoplasm of blastocysts, potentially improving bovine embryos.
Additional keywords: cAMP, gene expression, mass spectrometry.
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