Molecular cloning and epigenetic change detection of Kiss1 during seasonal reproduction in Chinese indigenous sheep
Xiaoyun He A , Qiuyue Liu A , Xiaoyu Li A , Xiaofei Guo A , Xiangyu Wang A , Wenping Hu A , Ran Di A B and Mingxing Chu A B
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
B Corresponding authors. Email: dirangirl@163.com; mxchu@263.net
Reproduction, Fertility and Development 30(5) 734-743 https://doi.org/10.1071/RD17028
Submitted: 27 January 2017 Accepted: 23 September 2017 Published: 15 November 2017
Abstract
Like most seasonal domesticated species, sheep are short-day breeders, which means that the reproduction axis is activated by short days. The annual photoperiodic cycle affects the amount of daylength information that is transmitted to the hypothalamic–pituitary–gonadal (HPG) axis by regulating pulsatile secretion of gonadotrophin-releasing hormone from the hypothalamus. Kisspeptin, which is encoded by Kiss1, plays a major role in reproductive seasonality. Based on results from our previous Solexa sequencing data obtained from Tan (T) and Small Tail Han (STH) sheep during anoestrus and the breeding season, full-length mRNA information for ovine Kiss1 was obtained; 894 bp in T sheep and 1145 bp in STH sheep. Both encode 135 amino acids. Additionally, T and STH sheep have different transcription start sites of Kiss1. Kiss1 expression during oestrus was significantly higher than that during dioestrus, both in T and STH sheep (P < 0.01). We also found a strong relationship between Kiss1 mRNA levels and histone H3 acetylation status in the 5′ promoter region of ovine Kiss1. These data indicated that epigenetic modification occurs during reproduction in sheep, and this is the first report that histone H3 deacetylation occurs in the hypothalamus of seasonal sheep breeders during the transition from dioestrus to oestrus.
Additional keywords: epigenetics, expression, histone acetylation, seasonal breeding.
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