Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation
Ido Bar A , Andre Smith A , Erin Bubner B , Goro Yoshizaki C , Yutaka Takeuchi D , Ryosuke Yazawa C , Ben Nan Chen E , Scott Cummins A and Abigail Elizur A F
+ Author Affiliations
- Author Affiliations
A Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, Qld 4558, Australia.
B Lincoln Marine Science Centre, School of Biological Science, Flinders University, PO Box 2023, Port Lincoln, SA 5606, Australia.
C Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
D Research Center for Advanced Science and Technology, Tokyo University of Marine and Science Technology, 670 Banda, Tateyama-shi, Chiba, 294-0308, Japan.
E Clean Seas Tuna Ltd, PO Box 159, Port Lincoln, SA 5606, Australia.
F Corresponding author. Email: aelizur@usc.edu.au
Reproduction, Fertility and Development 28(12) 2051-2064 https://doi.org/10.1071/RD15136
Submitted: 8 April 2015 Accepted: 11 June 2015 Published: 21 July 2015
Abstract
Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6–10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts’ gonads.
Additional keywords: aquaculture, dead-end, gonad development, spermatogenesis, vasa.
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