Genomic and non-genomic effects of progesterone on prostaglandin (PG) F2α and PGE2 production in the bovine endometrium
Mariko Kuse A , Ryosuke Sakumoto B and Kiyoshi Okuda A C
+ Author Affiliations
- Author Affiliations
A Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
B Reproductive Biology Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan.
C Corresponding author. Email: kokuda@okayama-u.ac.jp
Reproduction, Fertility and Development 28(10) 1588-1597 https://doi.org/10.1071/RD14490
Submitted: 10 December 2014 Accepted: 6 March 2015 Published: 21 April 2015
Abstract
Progesterone (P4) acts through different actuating pathways called genomic and non-genomic pathways. Here we investigated whether P4 regulates prostaglandin (PG) F2α (PGF) and PGE2 production in bovine endometrium through different pathways. Cultured endometrial cells were exposed to P4 for a short time (5–20 min) or bovine serum albumin (BSA)-conjugated P4 (P4-BSA) for 24 h. Progesterone treatment for 24 h stimulated PGE2 production in epithelial cells, but suppressed both PGF and PGE2 production and the expression of PG-metabolising enzymes including phospholipase A2 (PLA2) and cyclooxygenase-2 (COX2) in stromal cells. Short-term (5–20 min) P4 treatment did not affect PLA2 or COX2 transcript levels in either cell type. P4-BSA increased PGF and PGE2 production only in epithelial cells. Nuclear P4 receptor mRNA expression in endometrium was higher at the follicular phase than at the early- to mid-luteal stages, whereas membrane P4 receptor mRNA expression did not change throughout the oestrous cycle. The overall results suggest that P4 controls PG production by inhibiting enzymes via a genomic pathway and by stimulating signal transduction via a non-genomic pathway. Consequently, P4 may protect the corpus luteum by attenuating PGF production in stromal cells and by increasing PGE2 secretion from epithelial cells.
Additional keywords: cows, luteolysis, steroid hormone, uterus.
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