Regulation of primordial follicle recruitment by cross-talk between the Notch and phosphatase and tensin homologue (PTEN)/AKT pathways
Lin-Qing Wang A B , Jing-Cai Liu A C , Chun-Lei Chen A B , Shun-Feng Cheng A B , Xiao-Feng Sun A C , Yong Zhao A E , Shen Yin A B , Zhu-Mei Hou A D , Bo Pan E , Cheng Ding A B , Wei Shen A B and Xi-Feng Zhang A B F
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao 266109, China.
B College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
C College of Life Science, Qingdao Agricultural University, Qingdao 266109, China.
D College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
E Department of Animal and Poultry Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
F Corresponding author. Email: zhangxf9465@163.com
Reproduction, Fertility and Development 28(6) 700-712 https://doi.org/10.1071/RD14212
Submitted: 17 June 2014 Accepted: 11 September 2014 Published: 27 October 2014
Abstract
The growth of oocytes and the development of follicles require certain pathways involved in cell proliferation and survival, such as the phosphatidylinositol 3-kinase (PI3K) pathway and the Notch signalling pathway. The aim of the present study was to investigate the interaction between Notch and the PI3K/AKT signalling pathways and their effects on primordial follicle recruitment. When the Notch pathway was inhibited by L-685,458 or N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycinet-butyl ester (DAPT) in vitro, the expression of genes in the pathway and the percentage of oocytes in growing follicles decreased significantly in mouse ovaries. By 2 days postpartum, ovaries exposed to DAPT, short interference (si) RNA against Notch1 or siRNA against Hairy and enhancer of split-1 (Hes1) had significantly decreased expression of HES1, the target protein of the Notch signalling pathway. In contrast, expression of phosphatase and tensin homologue (Pten), a negative regulator of the AKT signalling pathway, was increased significantly. Co immunoprecipitation (Co-IP) revealed an interaction between HES1 and PTEN. In addition, inhibition of the Notch signalling pathway suppressed AKT phosphorylation and the proliferation of granulosa cells. In conclusion, the recruitment of primordial follicles was affected by the proliferation of granulosa cells and regulation of the interaction between the Notch and PI3K/AKT signalling pathways.
Additional keywords: granulosa cells, mice, ovary.
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