In vitro culture of stem-like cells derived from somatic cell nuclear transfer bovine embryos of the Korean beef cattle species, HanWoo
Daehwan Kim A , Sangkyu Park A , Yeon-Gil Jung B and Sangho Roh A C
+ Author Affiliations
- Author Affiliations
A Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University School of Dentistry, 101, Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea.
B ET Biotech Co. Ltd, 560-34, Ssarijae-ro, Jangsu-gun, Jeollabuk-do 597-851, Republic of Korea.
C Corresponding author. Email: sangho@snu.ac.kr
Reproduction, Fertility and Development 28(11) 1762-1780 https://doi.org/10.1071/RD14071
Submitted: 22 February 2014 Accepted: 12 April 2015 Published: 13 May 2015
Abstract
We established and maintained somatic cell nuclear transfer embryo-derived stem-like cells (SCNT-eSLCs) from the traditional Korean beef cattle species, HanWoo (Bos taurus coreanae). Each SCNT blastocyst was placed individually on a feeder layer with culture medium containing three inhibitors of differentiation (3i). Primary colonies formed after 2–3 days of culture and the intact colonies were passaged every 5–6 days. The cells in each colony showed embryonic stem cell-like morphologies with a distinct boundary and were positive to alkaline phosphatase staining. Immunofluorescence and reverse transcription–polymerase chain reaction analyses also confirmed that these colonies expressed pluripotent markers. The colonies were maintained over 50 passages for more than 270 days. The cells showed normal karyotypes consisting of 60 chromosomes at Passage 50. Embryoid bodies were formed by suspension culture to analyse in vitro differentiation capability. Marker genes representing the differentiation into three germ layers were expressed. Typical embryonal carcinoma was generated after injecting cells under the testis capsule of nude mice, suggesting that the cultured cells may also have the potential of in vivo differentiation. In conclusion, we generated eSLCs from SCNT bovine embryos, using a 3i system that sustained stemness, normal karyotype and pluripotency, which was confirmed by in vitro and in vivo differentiation.
Additional keywords: bovine, embryonic stem cell, small molecule.
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