In vitro manipulation techniques of porcine embryos: a meta-analysis related to transfers, pregnancies and piglets
Ying Liu A E , Juan Li A B , Peter Løvendahl C , Mette Schmidt D , Knud Larsen C and Henrik Callesen A
+ Author Affiliations
- Author Affiliations
A Reproductive Biology, Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark.
B College of Animal Science and Technology, Nanjing Agricultural University, 210095 Jiangsu Province, Nanjing Wei Gang No. 1, China.
C Department of Molecular Biology and Genetics, Aarhus University, DK-8830 Tjele, Denmark.
D Department of Large Animal Sciences, Faculty of Life Science, University of Copenhagen, DK-1870 Frederiksberg C, Denmark.
E Corresponding author. Email: ying.liu@agrsci.dk
Reproduction, Fertility and Development 27(3) 429-439 https://doi.org/10.1071/RD13329
Submitted: 26 September 2013 Accepted: 21 January 2014 Published: 24 February 2014
Abstract
During the last 17 years, considerable advancements have been achieved in the production of pigs, transgenic and non-transgenic, by methods of somatic cell nuclear transfer, in vitro fertilisation, intracytoplasmic sperm injection, microinjection and sperm-mediated gene transfer by artificial insemination. Therefore, a review of the overall efficiency for the developmental competence of embryos produced by these in vitro methods would be useful in order to obtain a more thorough overview of this growing area with respect to its development and present status. In this review a meta-analysis was used to analyse data collected from all published articles with a focus on zygotes and embryos for transfer, pregnancy, full-term development and piglets born. It was generally concluded that an increasing level of in vitro manipulation of porcine embryos decreased the overall efficiency for production of piglets. The techniques of nuclear transfer have been developed markedly through the increasing number of studies performed, and the results have become more stable. Prolonged in vitro culture period did not lead to any negative effect on nuclear transfer embryos after their transfer and it resulted in a similar or even higher litter size. More complete information is needed in future scientific articles about these in vitro manipulation techniques to establish a more solid basis for the evaluation of their status and to reveal and further investigate any eventual problems.
Additional keywords: nuclear transfer, pig, transgenic.
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