Assessment of DNA damage in goat preantral follicles after vitrification of the ovarian cortex
Luciana R. Faustino A F , Adeline A. Carvalho A , Cleidson M. G. Silva A , Rafael Rossetto A , Cláudio A. P. Lopes A , Maurício F. van Tilburg B , Pedro B. M. Carneiro C , Sônia N. Báo D , Arlindo A. A. Moura B , Vilceu Bordignon E , José R. Figueiredo A and Ana Paula R. Rodrigues A
+ Author Affiliations
- Author Affiliations
A Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE 60740-930, Brazil.
B Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Av. Mister Hull, s/n Campus do Pici, Fortaleza, CE 60021-970, Brazil.
C Institute of Marine Science (LABOMAR), Federal University of Ceará, Av. Abolição, 3207, Meireles, Fortaleza, CE 60165-081, Brazil.
D Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Campus Darcy Ribeiro, Asa Norte, Brasília, DF 70919-970, Brazil.
E Department of Animal Science, McGill University, 21,111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, H9X 3V9, Canada.
F Corresponding author. Email: lrfaustino@fulbrightmail.org
Reproduction, Fertility and Development 27(3) 440-448 https://doi.org/10.1071/RD13164
Submitted: 29 May 2013 Accepted: 23 November 2013 Published: 3 January 2014
Abstract
Effective methods for gamete preservation should have low impact on DNA integrity. The present study investigated the effects of vitrification of goat ovarian tissues on the occurrence of DNA fragmentation and DNA double-stand breaks using the terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL) assay and detection of phosphorylated histone H2AX (γH2AX), respectively. Goat ovaries were collected at a local abattoir and 12 tissue fragments were prepared from each ovarian pair. Tissue fragments were used as fresh control samples or were cultured in vitro, vitrified or vitrified and cultured. Vitrification was performed using the Ovarian Tissue Cryosystem. Fragments from all groups (control and treatments) were processed for histology, transmission electron microscopy, TUNEL assay and immunofluorescence. Compared with fresh control samples, a lower percentage of morphologically normal follicles was detected in the vitrification followed by culture treatment group (P < 0.05). Normal follicular ultrastructure was observed in all groups. Immunofluorescence revealed the presence of γH2AX foci in few oocytes and ovarian stromal cells. TUNEL-positive follicles were found in samples without significant differences among groups (P > 0.05). In conclusion, the vitrification protocol used in the present study did not increase DNA damage in preantral follicles enclosed in goat ovarian tissues.
Additional keywords: DNA fragmentation, γH2AX, oocyte, terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL) assay.
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