Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic
Giorgia Gioacchini A D , Luisa Dalla Valle B , Francesca Benato B , Gian Maria Fimia C , Roberta Nardacci C , Fabiola Ciccosanti C , Mauro Piacentini C , Andrea Borini D and Oliana Carnevali A E
+ Author Affiliations
- Author Affiliations
A Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy.
B Dipartimento di Biologia, Università di Padova, Via U.Bassi 58/B, 35121, Padova, Italy.
C Istituto Nazionale per le Malattie Infettive IRCCS ‘Lazzaro Spallanzani’, Via Portuense 292, 00149 Roma, Italy.
D Tecnobios Procreazione, Centre for Reproductive Health, Via Dante, 15 - 40125 Bologna, Italy.
E Corresponding author. Email: o.carnevali@univpm.it
Reproduction, Fertility and Development 25(8) 1115-1125 https://doi.org/10.1071/RD12187
Submitted: 1 March 2012 Accepted: 11 October 2012 Published: 30 November 2012
Abstract
The present study investigated autophagic processes in Danio rerio preovulatory follicles (Stage III and IV). There were more autophagosomes, as revealed by electron microscopy, in follicles from females fed the probiotic Lactobacillus rhamnosus IMC 501. This was confirmed by increased expression of genes involved in the autophagic process, namely ambra1, becn1, lc3 and uvrag. In addition, preovulatory follicles from females fed the probiotic contained more microtubule-associated protein 1 light chain 3 isoform II (LC3-II) and less p62 protein. The increased autophagy in preovulatory follicles from females fed the probiotic was concomitant with a decrease in the apoptotic process in the ovary, as evidenced by terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling analysis and confirmed by lower expression of genes involved in apoptosis (i.e. p53, bax, apaf and cas3) and higher expression as igfII and igf1r. The results of the present study provide preliminary evidence of the involvement of autophagy during follicle development in the zebrafish ovary. In addition, we have demonstrated for the first time that a functional food, such as L. rhamnosus IMC 501, can modulate the balance between apoptosis and autophagy that regulates ovary physiology in zebrafish by inhibiting follicular apoptosis and improving follicular survival.
Additional keywords : activating molecule in becn-1-regulated autophagy (Ambra1), Lactobacillus rhamnosus, zebrafish.
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