Quantitative analysis of sperm mRNA in the pig: relationship with early embryo development and capacitation
Jae Yeon Hwang A , Brendan P. Mulligan A , Hyung-Min Kim A , Byoung-Chul Yang B and Chang-Kyu Lee A C
+ Author Affiliations
- Author Affiliations
A Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Science, Seoul National University, Seoul 151-921, Korea.
B National Institute of Animal Science, Rural Department Administration, Suwon 441-706, Korea.
C Corresponding author. Email: leeck@snu.ac.kr
Reproduction, Fertility and Development 25(5) 807-817 https://doi.org/10.1071/RD12160
Submitted: 23 May 2012 Accepted: 5 July 2012 Published: 20 August 2012
Abstract
Although it is well known that mRNA is present in mammalian spermatozoa, the relevance of mRNA to capacitation and early embryo development in the pig remains unclear. In the present study, we investigated differences in the abundance of selected mRNAs coding for MYC, CYP19, ADAM2, PRM1 and PRM2 in purified porcine spermatozoa depending on embryo cleavage rate and capacitation (n = 20 semen samples). Semen samples were used in IVF procedures, with subsequent embryo development classified into one of two groups based on cleavage rate (i.e. high (>75%) and low (<75%) cleavage groups) and mRNA abundance in purified spermatozoa compared between these two groups. In addition, mRNA abundance was compared between capacitated and non-capacitated spermatozoa. Comparison of mRNA levels between porcine spermatozoa revealed that the abundance of MYC, CYP19, ADAM2, PRM1 and PRM2 mRNA was significantly greater in the high cleavage group (n = 10 high cleavage group semen samples) than in the low cleavage group (n = 10; P < 0.05). Significant downregulation of MYC mRNA was observed in capacitated spermatozoa (n = 12; P < 0.05). The results of the present study suggest that the amount of specific mRNAs could be used for estimating the quality of spermatozoa in the pig.
Additional keywords: IVF, RNA, spermatozoa.
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