Transcriptional heterogeneity in mouse embryonic stem cells
Tetsuya S. Tanaka
+ Author Affiliations
- Author Affiliations
Department of Animal Sciences, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL 61801, USA. Email: ttanaka@illinois.edu
Reproduction, Fertility and Development 21(1) 67-75 https://doi.org/10.1071/RD08219
Published: 9 December 2008
Abstract
The embryonic stem (ES) cell is a stem cell derived from early embryos that can indefinitely repeat self-renewing cell division cycles as an undifferentiated cell in vitro and give rise to all specialised cell types in the body. However, manipulating ES cell differentiation in vitro is a challenge due to, at least in part, heterogeneous gene induction. Recent experimental evidence has demonstrated that undifferentiated mouse ES cells maintained in culture exhibit heterogeneous expression of Dppa3, Nanog, Rex1, Pecam1 and Zscan4 as well as genes (Brachyury/T, Rhox6/9 and Twist2) normally expressed in specialised cell types. The Nanog-negative, Rex1-negative or T-positive ES cell subpopulation has a unique differentiation potential. Thus, studying the mechanism that generates ES cell subpopulations will improve manipulation of ES cell fate and help our understanding of the nature of embryonic development.
Acknowledgement
The author extends special thanks to Dr Matthew B. Wheeler for critical reading of this manuscript. In addition, the author thanks his mentors, who have trained him, and his colleagues for providing support, encouragement and inspiration.
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