DNA methylation patterns in in vitro-fertilised goat zygotes
J. Hou A , T. H. Lei A , L. Liu A , X. H. Cui A , X. R. An A B and Y. F. Chen AA State Key Laboratory for Agrobiotechnology and Department of Animal Physiology, College of Biological Science, China Agricultural University, Beijing 100094, China.
B Corresponding author. Email: xra@cau.edu.cn
Reproduction, Fertility and Development 17(8) 809-813 https://doi.org/10.1071/RD05075
Submitted: 4 July 2005 Accepted: 25 September 2005 Published: 16 December 2005
Abstract
Recent studies have shown that zygote demethylation patterns in mammals are variable among species. However, the methylation patterns of goat zygotes have not yet been reported on. In the present study, using immunofluorescence staining with an antibody against 5-methylcytosine, the methylation patterns of in vitro-derived goat zygotes were studied. The results indicate that goat zygotes do not undergo active global demethylation during pronuclei development, which is similar to the situation in ovine, but not in murine or bovine zygotes. This is believed to be the first report regarding methylation in goat zygotes.
Acknowledgments
The authors thank Drs Huanmin Zhou and Jinquan Li (Inner Mongolian Agricultural University) for their kind assistance with embryo production, and Drs Dongjun Liu and Ming Cang (Inner Mongolian University) for their expert help with the confocal microscopy. This research was supported by a competitive research grant from the Natural Scientific Foundation of China (no. 30270956).
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