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RESEARCH ARTICLE

Heavy metals risk assessment in Salmo letnica from Lake Ohrid in Albania

Patrizia Marchetti A D , Fatmira Shehu B , Angela Di Pinto A , Valeriana Colao C , Giuseppina Tantillo A and Edmondo Ceci A
+ Author Affiliations
- Author Affiliations

A Department of Veterinary Medicine, University of Bari Aldo Moro, Strada Provinciale per Casamassima, chilometro 3, I-70010 Valenzano, Bari, Italy.

B Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kodër-Kamëz, SH1, AL-1000 Tirana, Albania.

C Association of Apulian Environmental Biologists (ABAP), Via Giulio Petroni 15/F, I-70124 Bari, Italy.

D Corresponding author. Email: patrizia.marchetti@uniba.it

Marine and Freshwater Research 70(11) 1543-1551 https://doi.org/10.1071/MF18419
Submitted: 31 October 2018  Accepted: 22 March 2019   Published: 30 May 2019

Abstract

The Ohrid trout (Salmo letnica) is an endemic species of trout found only in Lake Ohrid, in the Balkan republics of North Macedonia and Albania, where it is a species of significant economic and nutritional interest. Considering the importance of chemical risk assessment in fish for human consumption set out by European and international legislation and the risk of extinction of the Ohrid trout, the aim of the present study was to evaluate the safety of Ohrid trout flesh for human consumption by determining the heavy metal concentration in different organs through atomic absorption spectrometry (AAS). Heavy metal concentrations differed significantly in different organs (P < 0.001). The mean and range of Pb and Cr concentrations in muscle were lower than the permissible limits recommended for human consumption according to the European Union (EU), as well as the Food and Agriculture Organization of the United Nations (FAO) and World Health Organization, whereas the mean concentration and range of Cd in muscle tissue were above the critical limits for human consumption set by the EU and FAO, revealing inspection-related problems. Accordingly, S. letnica is not completely safe for human consumption. Therefore, the long-term assessment of heavy metal concentrations in Ohrid trout from Lake Ohrid is an important safety measure for fish consumers and will help assess the status of exploitation of this biological resource.

Additional keywords: environmental contaminants.


References

Abu Hilal, A. H., and Ismail, N. S. (2008). Heavy metals in eleven common species of fish from the Gulf of Aqaba, Red Sea. Jordan Journal of Biological Sciences 1, 13e8.

Acosta, I. B., Varela-Junior, A. S., Fernandes, E., Fenandes, S., Figueiredo-Cardoso, T., Schwartz-Caldas, J., Desessards-Jardim, R., and Dahl Corcini, C. (2016). Effects of exposure to cadmium in sperm cells of zebrafish, Danio rerio. Toxicology Reports 3, 696–700.
Effects of exposure to cadmium in sperm cells of zebrafish, Danio rerio.Crossref | GoogleScholarGoogle Scholar | 28959594PubMed |

Akpinar, M. A., Görgün, S., and Akpinar, A. E. (2009). A comparative analysis of the fatty acid profiles in the liver and muscles of male and female Salmo trutta macrostigma. Food Chemistry 112, 6–8.
A comparative analysis of the fatty acid profiles in the liver and muscles of male and female Salmo trutta macrostigma.Crossref | GoogleScholarGoogle Scholar |

Al-Busaidi, M., Yesudhason, P., Al-Mughairi, S., Al-Rahbi, W., Al-Harthy, K. S., Al-Mazrooei, N. A., and Al-Habsi, S. H. (2011). Toxic metals in commercial marine fish in Oman with reference to national and international standards. Chemosphere 85, 67–73.
Toxic metals in commercial marine fish in Oman with reference to national and international standards.Crossref | GoogleScholarGoogle Scholar | 21700309PubMed |

Albrecht, C., and Wilke, T. (2008). Ancient Lake Ohrid: biodiversity and evolution. Hydrobiologia 615, 103–140.
Ancient Lake Ohrid: biodiversity and evolution.Crossref | GoogleScholarGoogle Scholar |

Amiard, J. C., Amiard-Triquet, C., Barka, S., Pellerin, J., and Rainbow, P. S. (2006). Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquatic Toxicology (Amsterdam, Netherlands) 76, 160–202.
Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers.Crossref | GoogleScholarGoogle Scholar |

Arockia Vasanthi, L., Muruganandam, A., Revathi, P., Baskar, B., Jayapriyan, K., Baburajendran, R., and Munuswamy, N. (2014). The application of histo-cytopathological biomarkers in the mud crab Scylla serrata (Forskal) to assess heavy metal toxicity in Pulicat Lake, Chennai. Marine Pollution Bulletin 81, 85–93.
The application of histo-cytopathological biomarkers in the mud crab Scylla serrata (Forskal) to assess heavy metal toxicity in Pulicat Lake, Chennai.Crossref | GoogleScholarGoogle Scholar | 24635984PubMed |

Arulkumar, A., Paramasivam, S., and Rajaram, R. (2017). Toxic heavy metals in commercially important food fishes collected from Palk Bay, southeastern India. Marine Pollution Bulletin 119, 454–459.
Toxic heavy metals in commercially important food fishes collected from Palk Bay, southeastern India.Crossref | GoogleScholarGoogle Scholar | 28385512PubMed |

Avenant-Oldewage, A., and Marx, H. M. (2000). Bioaccumulation of chromium, copper and iron in the organs and tissues of Clarias gariepinus in the Olifants River, Kruger National Park. Water SA 26, 569–582.

Avramoski, O., Kycyku, S., Naumoski, T., Panovski, D., Puka, V., Selfo, L., and Watzin, M. (2003). Lake Ohrid, Macedonia and Albania management experience and lessons learned brief. Draft Final 29Dec03. Available at http://www.worldlakes.org/uploads/Ohrid_12.29.03.pdf [Verified 17 April 2019].

Batvari, B. P., Kamala-Kannan, S., Shanthi, K., Krishnamoorthy, R., Lee, K. J., and Jayaprakash, M. (2008). Heavy metals in two fish species (Carangoidel malabaricus and Belone stronglurus) from Pulicat Lake, north of Chennai, Southeast Coast of India. Environmental Monitoring and Assessment 145, 167–175.
Heavy metals in two fish species (Carangoidel malabaricus and Belone stronglurus) from Pulicat Lake, north of Chennai, Southeast Coast of India.Crossref | GoogleScholarGoogle Scholar | 18040878PubMed |

Bode, A., Zoga, P., Xhulaj, D., and Xhulaj, S. (2010). Mining residues around Lake Ohrid. Journal of Mining and Metallurgy 46 A, 23–31.

Canli, M., and Atli, G. (2003). The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environmental Pollution 121, 129–136.
The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species.Crossref | GoogleScholarGoogle Scholar | 12475070PubMed |

Castro-González, M. I., and Méndez-Armenta, M. (2008). Heavy metals: implications associated to fish consumption. Environmental Toxicology and Pharmacology 26, 263–271.
Heavy metals: implications associated to fish consumption.Crossref | GoogleScholarGoogle Scholar | 21791373PubMed |

Chourpagar, A. R., and Kulkarn, G. K. (2011). Heavy metal toxicity to a freshwater crab, Barytelphus acunicularis (Westwood) from Aurangabad region. Recent Research in Science and Technology 3, 1–5.

Commission of the European Communities (2006). Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union – Legislation 364, 5–24.

Commission of the European Communities (2008). No 629/2008 of 2 July 2008 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union – Legislation 173, 6–9.

Council of the European Communities (1992). Council Directive 92/43/EEC of 21 May 1992 on the conservation of natural habitats and of wild fauna and flora. Official Journal of the European Communities – Legislation 206, 7–50.

Crivelli, A. J. (2006). Salmo letnica. In ‘The IUCN Red List of Threatened Species 2006’, e.T19858A9039230. (International Union for Conservation of Nature and Natural Resources.) Available at http://www.iucnredlist.org/details/19858/0 [Verified 10 May 2019].

Cullaj, A., Celo, V., and Babi, D. (2000). Analytical investigation of heavy metal content in sediments of Albanian coast. Journal of Environmental Protection and Ecology 1, 81–90.

Cullaj, A., Hasko, A., Miho, A., Schanz, F., Brandl, H., and Bachofen, R. (2005). The quality of Albanian natural waters and the human impact. Environment International 31, 133–146.
The quality of Albanian natural waters and the human impact.Crossref | GoogleScholarGoogle Scholar | 15607787PubMed |

Dhaneesh, K. V., Gopi, M., Ganeshamurthy, R., Kumar, T. T. A., and Balasubramanian, T. (2012). Bio-accumulation of metals on reef associated organisms of Lakshadweep Archipelago. Food Chemistry 131, 985–991.
Bio-accumulation of metals on reef associated organisms of Lakshadweep Archipelago.Crossref | GoogleScholarGoogle Scholar |

Dural, M., Goksu, M. Z. L., and Ozak, A. A. (2007). Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon. Food Chemistry 102, 415–421.
Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon.Crossref | GoogleScholarGoogle Scholar |

Eisler, R. (2010). ‘Compendium of Trace Metals and Marine Biota. Vol. 2: Vertebrates.’ (Elsevier: Amsterdam, Netherlands.)

El-Moselhy, K. M., Othman, A. I., Abd El-Azem, H., and El-Metwally, M. E. A. (2014). Bioaccumulation of heavy metals in some tissues of fish in the Red Sea, Egypt. Egyptian Journal of Basic and Applied Sciences 1, 97–105.
Bioaccumulation of heavy metals in some tissues of fish in the Red Sea, Egypt.Crossref | GoogleScholarGoogle Scholar |

Elnabris, K. J., Muzyed, S. K., and El-Ashgar, N. M. (2013). Heavy metal concentrations in some commercially important fishes and their contribution to heavy metals exposure in Palestinian people of Gaza Strip (Palestine). Journal of the Association of Arab Universities for Basic and Applied Sciences 13, 44–51.
Heavy metal concentrations in some commercially important fishes and their contribution to heavy metals exposure in Palestinian people of Gaza Strip (Palestine).Crossref | GoogleScholarGoogle Scholar |

European Commission (2014). Commission Regulation (EU) No 488/2014 of 12 May 2014 Amending Regulation (EC) No 1881/2006 as regards maximum levels of cadmium in foodstuffs. Official Journal of the European Union – Legislation 138, 75–79.

European Food Safety Authority (2009). Scientific opinion of the Panel on Contaminants in the Food Chain on a request from the European Commission on cadmium in food. EFSA Journal 980, 1–139.

European Food Safety Authority (2012). Cadmium dietary exposure in the European population. EFSA Journal 10, 2551.
Cadmium dietary exposure in the European population.Crossref | GoogleScholarGoogle Scholar |

European Parliament and the Council of the European Union (2002). Regulation (EC) No 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety. Official Journal of the European Communities – Legislation 31, 1–24.

European Parliament and the Council of the European Union (2013). Regulation (EU) No 1380/2013 of the European Parliament and of the Council of 11 December 2013 on the Common Fisheries Policy, amending Council Regulations (EC) No 1954/2003 and (EC) No 1224/2009 and repealing Council Regulations (EC) No 2371/2002 and (EC) No 639/2004 and Council Decision 2004/585/EC. Official Journal of the European Communities – Legislation 354, 22–61.

European Parliament and the Council of the European Union (2015). Regulation (EU) 2015/812 of the European Parliament and of the Council of 20 May 2015 amending Council Regulations (EC) No 850/98, (EC) No 2187/2005, (EC) No 1967/2006, (EC) No 1098/2007, (EC) No 254/2002, (EC) No 2347/2002 and (EC) No 1224/2009, and Regulations (EU) No 1379/2013 and (EU) No 1380/2013 of the European Parliament and of the Council, as regards the landing obligation, and repealing Council Regulation (EC) No 1434/98. Official Journal of the European Communities – Legislation 133, 1–20.

Ezejiofor, T. I. N., Udebuani, A. C., Ezeji, E. U., Ayalogbu, E. A., Azuwuike, C. O., Ezejiofor, A. N., and Ngwogu, K. O. (2013). Environmental metals pollutants load of a densely populated and heavily industrialized commercial city of Aba Nigeria. Journal of Toxicology and Environmental Health Sciences 5, 1–11.
Environmental metals pollutants load of a densely populated and heavily industrialized commercial city of Aba Nigeria.Crossref | GoogleScholarGoogle Scholar |

Food and Agriculture Organization (1983). Compilation of legal limits for hazardous substances in fish and fishery products. FAO Fisheries Circular 764, 102.

Food and Agriculture Organization and World Health Organization (1989). ‘National Research Council Recommended Dietary Allowances’, 10th edn. (National Academy Press: Washington DC, USA.)

Froese, R., and Pauly, D. (2017). Salmo letnica (Karaman, 1924). In ‘FishBase’. Available at http://www.fishbase.se/summary/Salmo-letnica.html [Verified 11 October 2017].

Galay-Burgos, M., and Rainbow, P. S. (2001). Availability of cadmium and zinc from sewage sludge to flounder Platichthys flesus, via marine food chain. Marine Environmental Research 51, 417–439.
Availability of cadmium and zinc from sewage sludge to flounder Platichthys flesus, via marine food chain.Crossref | GoogleScholarGoogle Scholar |

Hoxhaj, M. (2016). Assessment of impact of Chervenak and Pojska mines on Ohrid Lake and its watershed basin. Ph.D. Thesis, Polytechnic University of Tirana, Faculty of Geology and Mines, Tirana, Albania. Available at http://dibmin-fgjm.org/doktorata/DisertacioniMHoxhaj.pdf [Verified 17 April 2019].

Kargin, F. (1998). Metal concentrations in tissues of the freshwater fish Capoeta barroisi from the Seyhan River (Turkey). Bulletin of Environmental Contamination and Toxicology 60, 822–828.
Metal concentrations in tissues of the freshwater fish Capoeta barroisi from the Seyhan River (Turkey).Crossref | GoogleScholarGoogle Scholar | 9595202PubMed |

Karunanidhi, K., Rajendran, R., Pandurangan, D., and Arumugam, G. (2017). First report on distribution of heavy metals and proximate analysis in marine edible puffer fishes collected from Gulf of Mannar Marine Biosphere Reserve, South India. Toxicology Reports 4, 319–327.
First report on distribution of heavy metals and proximate analysis in marine edible puffer fishes collected from Gulf of Mannar Marine Biosphere Reserve, South India.Crossref | GoogleScholarGoogle Scholar | 28959655PubMed |

Koizumi, K., and Hiratsuka, S. (2009). Fatty acid compositions in muscles of wild and cultured ocellate puffer Takifugu rubripes. Fisheries Science 75, 1323–1328.
Fatty acid compositions in muscles of wild and cultured ocellate puffer Takifugu rubripes.Crossref | GoogleScholarGoogle Scholar |

Kostoski, G., Albrecht, C., Trajanovski, S., and Wilke, T. (2010). A freshwater biodiversity hotspot under pressure – assessing threats and identifying conservation needs for ancient Lake Ohrid. Biogeosciences 7, 3999–4015.
A freshwater biodiversity hotspot under pressure – assessing threats and identifying conservation needs for ancient Lake Ohrid.Crossref | GoogleScholarGoogle Scholar |

Kulawik, P., Migdal, W., Gambus, F., Cieslik, E., Ozugul, F., Tkaczewska, J., Szczurowska, K., and Walkowska, I. (2016). Microbiological and chemical safety concerns regarding frozen fillets obtained from Pangasius sutchi and Nile tilapia exported to European countries. Journal of the Science of Food and Agriculture 96, 1373–1379.
Microbiological and chemical safety concerns regarding frozen fillets obtained from Pangasius sutchi and Nile tilapia exported to European countries.Crossref | GoogleScholarGoogle Scholar | 25907121PubMed |

Kumar, K. A., and Achyuthan, H. (2005). Heavy metal accumulation in certain marine animals along the east coast of Chennai Tamil Nadu, India. Journal of Environmental Biology 28, 637–643.

Kwon, Y. T., and Lee, C. W. (1998). Application of multiple ecological risk indices for the evaluation of heavy metal contamination in a coastal dredging area. The Science of the Total Environment 214, 203–210.
Application of multiple ecological risk indices for the evaluation of heavy metal contamination in a coastal dredging area.Crossref | GoogleScholarGoogle Scholar |

Malaj, E., Rousseau, D. P. L., Du Laing, G., and Lens, P. N. L. (2012). Near-shore distribution of heavy metals in the Albanian part of Lake Ohrid. Environmental Monitoring and Assessment 184, 1823–1839.
Near-shore distribution of heavy metals in the Albanian part of Lake Ohrid.Crossref | GoogleScholarGoogle Scholar | 21541777PubMed |

Matzinger, A., Spirkovski, Z., Patceva, S., and Wüest, A. (2006). Sensitivity of ancient Lake Ohrid to local anthropogenic impacts and global warming. Journal of Great Lakes Research 32, 158–179.
Sensitivity of ancient Lake Ohrid to local anthropogenic impacts and global warming.Crossref | GoogleScholarGoogle Scholar |

Matzinger, A., Schmid, M., Veljanoska-Sarafiloska, E., Patceva, S., Guseska, D., Wagner, B., Müller, B., Sturm, M., and Wüest, A. (2007). Eutrophication of ancient Lake Ohrid: global warming amplifies detrimental effects of increased nutrient inputs. Limnology and Oceanography 52, 338–353.
Eutrophication of ancient Lake Ohrid: global warming amplifies detrimental effects of increased nutrient inputs.Crossref | GoogleScholarGoogle Scholar |

Ministry of Agriculture, Fisheries and Food (2000). Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal of wastes at sea, 1997. Aquatic Environment Monitoring Report number 52, Center for Environment, Fisheries and Aquaculture Science, Lowestoft, UK.

Mohammed, E., Mohammed, T., and Mohammed, A. (2017). Optimization of an acid digestion procedure for the determination of Hg, As, Sb, Pb and Cd in fish muscle tissue. MethodsX 4, 513–523.
Optimization of an acid digestion procedure for the determination of Hg, As, Sb, Pb and Cd in fish muscle tissue.Crossref | GoogleScholarGoogle Scholar | 29264277PubMed |

Moore, J. W., and Ramamoorthy, S. (1984). ‘Heavy Metals in Natural Waters: Applied Monitoring and Impact Assessment.’ (Springer-Verlag: New York, NY, USA.)

Oyakhilome, G. I., Aiyesanmi, A. F., Adefemi, S. O., and Asaolu, S. S. (2013). Heavy metals concentration in sediment and fish samples from Owena multi-purpose dam, Ondo State, southern Nigeria. British Journal of Applied & Technology 3, 65–76.
Heavy metals concentration in sediment and fish samples from Owena multi-purpose dam, Ondo State, southern Nigeria.Crossref | GoogleScholarGoogle Scholar |

Paquin, P. R., Gorsuch, J. W., Apte, S., Batley, G. E., Bowles, K. C., Campbell, P. G. C., Delos, C. G., Di Toro, D. M., Dwyer, R. L., Galvez, F., Gensemer, R. W., Goss, G. G., Hogstrand, C., Janssen, C. R., McGeer, J. C., Naddy, R. B., Playle, R. C., Santore, R. C., Schneider, U., Stubblefield, W. A., Wood, C. M., and Wu, K. B. (2002). The biotic ligand model: a historical overview. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP 133, 3–35.
The biotic ligand model: a historical overview.Crossref | GoogleScholarGoogle Scholar |

Pereira, L. S., Ribas, J. L. C., Vicari, T., Silva, S. B., Stival, J., Baldan, A. P., Valdez Domingos, F. X., Grassi, M. T., Cestari, M. M., and Silva de Assis, H. C. (2016). Effects of ecologically relevant concentrations of cadmium in a freshwater fish. Ecotoxicology and Environmental Safety 130, 29–36.
Effects of ecologically relevant concentrations of cadmium in a freshwater fish.Crossref | GoogleScholarGoogle Scholar | 27062343PubMed |

Qadir, A., and Malik, R. N. (2011). Heavy metals in eight edible fish species from two polluted tributaries (Aik and Palkhu) of the River Chenab, Pakistan. Biological Trace Element Research 143, 1524–1540.
Heavy metals in eight edible fish species from two polluted tributaries (Aik and Palkhu) of the River Chenab, Pakistan.Crossref | GoogleScholarGoogle Scholar | 21424780PubMed |

Rahman, M. S., Molla, A. H., Saha, N., and Rahman, A. (2012). Study on heavy metals levels and its risk assessment in some edible fishes from Bangshi River, Savar, Dhaka, Bangladesh. Food Chemistry 134, 1847–1854.
Study on heavy metals levels and its risk assessment in some edible fishes from Bangshi River, Savar, Dhaka, Bangladesh.Crossref | GoogleScholarGoogle Scholar | 23442629PubMed |

Raja, P., Veerasingam, S., Suresh, G., Marichamy, G., and Venkatachalapathy, R. (2009). Heavy metals concentration in four commercially valuable marine edible fish species from Parangipettai Coast, east coast of India. International Journal of Animal and Veterinary Advances 1, 10–14.

Rakaj, N. (1995). Conservation status of freshwater fish of Albania. Biological Conservation 72, 195–199.
Conservation status of freshwater fish of Albania.Crossref | GoogleScholarGoogle Scholar |

Roesijadi, G., and Robinson, W. E. (1994). Metal regulation in aquatic animals: mechanism of uptake, accumulation and release. In ‘Aquatic Toxicology Molecular, Biochemical and Cellular Perspectives’. (Eds D. C. Malins and G. K. Ostrander.) pp. 387–420. (Lewis Publishers: London, UK.)

Sallaku, F., Kovaci, V., and Shallari, S. (2002). ‘The Environmental Situation in Albania: Problems and Prospects for the Future. Technological and Economic Aspects of soil Bio/Phyto Remediation.’ (International Center for Science and High Technology and United Nations Industrial Development Organization: Trieste, Italy)

Sankar, T. V., and Ashok Kumar, K. (2014). Food safety with special reference to seafood. In ‘Practical Aspects of Seafood Safety’. (Eds S. K. Panda, F. Hassan, S. Sanjeev, and T. V. Sankar.) pp. 1–4. (CIFT: Kochi, India.)

Sankar, T. V., Zynudheen, A. A., Anandan, R., and Viswanathan Nair, P. G. (2006). Distribution of organo-chlorine pesticides and heavy metal residues in fish and shellfish from Calicut region, Kerala, India. Chemosphere 65, 583–590.
Distribution of organo-chlorine pesticides and heavy metal residues in fish and shellfish from Calicut region, Kerala, India.Crossref | GoogleScholarGoogle Scholar | 16678236PubMed |

Santore, R. C., Di Toro, D. M., Paquin, P. R., Allen, H. E., and Meyer, J. S. (2001). Biotic ligand model of the acute toxicity of metals. 2. Application to acute copper toxicity in freshwater fish and Daphnia. Environmental Toxicology and Chemistry 20, 2397–2402.
Biotic ligand model of the acute toxicity of metals. 2. Application to acute copper toxicity in freshwater fish and Daphnia.Crossref | GoogleScholarGoogle Scholar | 11596775PubMed |

Sarkar, T., Masihul Alam, M., Parvin, N., Fardous, Z., Chowdhury Alamgir, Z., Hossain, S., Haque, M. E., and Biswas, N. (2016). Assessment of heavy metals contamination and human health risk in shrimp collected from different farms and rivers at Khulna–Satkhira region, Bangladesh. Toxicology Reports 3, 346–350.
Assessment of heavy metals contamination and human health risk in shrimp collected from different farms and rivers at Khulna–Satkhira region, Bangladesh.Crossref | GoogleScholarGoogle Scholar | 28959555PubMed |

Sivaperumal, P., Sankar, T. V., and Viswanathan Nair, P. G. (2007). Heavy metal concentration in fish, shellfish and fish products from internal markets of India vis-a-vis international standards. Food Chemistry 102, 612–620.
Heavy metal concentration in fish, shellfish and fish products from internal markets of India vis-a-vis international standards.Crossref | GoogleScholarGoogle Scholar |

Sušnik, S., Snoj, A., Wilson, I. F., Mrdak, D., and Weiss, S. (2007). Historical demography of brown trout (Salmo trutta) in the Adriatic drainage including the putative S. letnica endemic to Lake Ohrid. Molecular Phylogenetics and Evolution 44, 63–76.
Historical demography of brown trout (Salmo trutta) in the Adriatic drainage including the putative S. letnica endemic to Lake Ohrid.Crossref | GoogleScholarGoogle Scholar | 17046289PubMed |

Turchini, G. M., Mentasti, T., Frøyland, L., Orban, E., Caprino, F., Moretti, V. M., and Valfre, F. (2003). Effects of alternative dietary lipid sources on performance, tissue chemical composition, mitochondrial fatty acid oxidation capabilities and sensory characteristics in brown trout (Salmo trutta L.). Aquaculture 225, 251–267.
Effects of alternative dietary lipid sources on performance, tissue chemical composition, mitochondrial fatty acid oxidation capabilities and sensory characteristics in brown trout (Salmo trutta L.).Crossref | GoogleScholarGoogle Scholar |

US Food and Drug Administration (1993). ‘Guidance Document for Chromium in Shellfish.’ (US Department of Health and Human Services, Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Affairs, Office of Seafood: Washington, DC, USA.)

Velusamy, A., Satheesh Kumar, P., and Anirudh Ram, S. (2014). Chinnadurai bioaccumulation of heavy metals in commercially important marine fishes from Mumbai Harbor, India. Marine Pollution Bulletin 81, 218–224.
Chinnadurai bioaccumulation of heavy metals in commercially important marine fishes from Mumbai Harbor, India.Crossref | GoogleScholarGoogle Scholar | 24631401PubMed |

Vogel, H., Wessels, M., Albrecht, C., Stich, H. B., and Wagner, B. (2010). Spatial variability of recent sedimentation in Lake Ohrid (Albania–Macedonia) – a complex interplay of natural and anthropogenic factors and their possible impact on biodiversity patterns. Biogeosciences 7, 3911–3930.
Spatial variability of recent sedimentation in Lake Ohrid (Albania–Macedonia) – a complex interplay of natural and anthropogenic factors and their possible impact on biodiversity patterns.Crossref | GoogleScholarGoogle Scholar |

World Health Organization (1989). Heavy metals – environmental aspects. Environment health criteria. Number 85. Lead – environmental aspects. International Programme on Chemical Safety. (WHO: Geneva, Switzerland.) Available at http://www.inchem.org [Verified 9 July 2018].

Yılmaz, A. B. (2003). Levels of heavy metals (Fe, Cu, Ni, Cr, Pb, and Zn) in tissue of Mugil cephalus and Trachurus mediterraneus from Iskenderun Bay, Turkey. Environmental Research 92, 277–281.
Levels of heavy metals (Fe, Cu, Ni, Cr, Pb, and Zn) in tissue of Mugil cephalus and Trachurus mediterraneus from Iskenderun Bay, Turkey.Crossref | GoogleScholarGoogle Scholar | 12804525PubMed |

Zhao, S., Feng, C., Quan, W., Chen, X., Niu, J., and Shen, Z. (2012). Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China. Marine Pollution Bulletin 64, 1163–1171.
Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China.Crossref | GoogleScholarGoogle Scholar | 22551849PubMed |