Relative abundance of Mycobacterium in ovine Johne’s disease
Andy O Leu A , Paul Pavli B C , David M Gordon A , Jeff Cave D , Jacek M Gowzdz E , Nick Linden D , Grant Rawlin E , Gwen E Allison A C and Claire L O’Brien A B C FA Research School of Biology, Australian National University, Acton, ACT 0200, Australia
B Gastroenterology and Hepatology Unit, Canberra Hospital, Garran, ACT 2605, Australia
C Medical School, Australian National University, Acton, ACT 0200, Australia
D Department of Environment and Primary Industries, Wodonga, Vic. 3690, Australia
E AgriBio, Bundoora, Vic. 3083, Australia
F Corresponding author. Tel: +61 2 6244 4023, Email: claire.obrien@anu.edu.au
Microbiology Australia 36(1) 32-36 https://doi.org/10.1071/MA15010
Published: 6 March 2015
Abstract
No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johne's disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.
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