A molecular perspective on the systematics of the spider crab genus Libinia Leach, 1815 (Majoidea : Epialtidae)
Ana Francisca Tamburus
A B and Fernando Luis Mantelatto A
+ Author Affiliations
- Author Affiliations
A Laboratory of Bioecology and Crustacean Systematics (LBSC), Department of Biology, Faculty of Philosophy, Science and Letters at Ribeirão Preto (FFCLRP), University of São Paulo (USP), Avenida Bandeirantes 3900, 14040-901, Ribeirão Preto (SP), Brazil. Email: flmantel@usp.br
B Corresponding author. Email: anaftg@yahoo.com.br
Invertebrate Systematics 35(6) 688-700 https://doi.org/10.1071/IS20085
Submitted: 25 November 2020 Accepted: 9 February 2021 Published: 13 August 2021
Abstract
Libinia, a genus of spider crabs, encompasses 10 valid species, three from the eastern Pacific and seven from the western Atlantic. Previous phylogenetic hypotheses based on molecular and larval morphological data suggested an uncertain position of the genus Libinia within the family Epialtidae. Therefore, we investigated the evolutionary relationships among the species of Libinia, and also suggested a position for this genus within the family. Phylogenetic trees were obtained from concatenated mtDNA sequences of 16S and COI fragments. Molecular dating methods were used to estimate the relative timing of origin and diversification within Libinia. Eighteen new primers were designed, and PCR conditions were standardised to amplify the COI fragments of older or poorly preserved pinned specimens, generating ~550 base pair sequences. Phylogenetic hypotheses supported the monophyly of the genus. Species that occur in the eastern Pacific and the ones found in the western Atlantic do not form monophyletic clades related to this geographical separation. In the eastern Pacific, there is low phylogenetic proximity between Libinia mexicana and L. setosa; and since we did not sample L. peruana, we inferred that it should cluster with L. setosa or L. mexicana due to morphological similarity. Libinia spinosa is separate from the other species, L. mexicana was a sister group of L. cavirostris, and L. ferreirae was closely related to L. dubia and L. rhomboidea, which occur in the Gulf of Mexico and in the Caribbean, respectively. In the Gulf of Mexico, L. dubia and L. emarginata are closely related. Although L. erinacea was not sampled, we suggest, from previous studies, that it should group with L. dubia due to morphological similarities. The phylogenetic hypothesis suggests the monophyly of Libinia, with its origins at the late Eocene. Finally, the recovered clades based on two molecular markers agreed with a parallel, linked morphological study that is still in progress.
Keywords: COI, decorator crabs, majoid, molecular phylogeny, 16S rRNA, small primers
References
Abele, L. G., and Kim, W. (1986). An illustrated guide to the marine decapod crustaceans of Florida. Technical Series 8, 1–760.Artimo, P., Jonnalagedda, M., Arnold, K., Baratin, D., Csardi, G., Castro, E., Duvaud, S., Flegel, V., Fortier, A., Gasteiger, E., Grosdidier, A., Hernandez, C., Ioannidis, V., Kuznetsov, D., Liechti, R., Moretti, S., Mostaguir, K., Redaschi, N., Rossier, G., Xenarios, I., and Stockinger, H. (2012). ExPASy: SIB bioinformatics resource portal. Nucleic Acids Research 40, W597–W603.
| ExPASy: SIB bioinformatics resource portal.Crossref | GoogleScholarGoogle Scholar | 22661580PubMed |
Bacon, C. D., Mora, A., Wagner, W. L., and Jaramillo, C. A. (2013). Testing geological models of the Isthmus of Panama in a phylogenetic framework. Botanical Journal of the Linnean Society 171, 287–300.
| Testing geological models of the Isthmus of Panama in a phylogenetic framework.Crossref | GoogleScholarGoogle Scholar |
Bouckaert, R., Vaughan, T. G., Barido-Sottani, J., Duchêne, S., Fourment, M., Gavryushkina, A., Heled, J., Jones, G., Kühnert, D., De Maio, N., Matschiner, M., Mendes, F. K., Müller, N. F., Ogilvie, H. A., Plessis, L., Popinga, A., Rambaut, A., Rasmussen, D., Siveronil, I., Suchard, M. A., Wu, C. H., Xie, D., Zhang, C., Stadler, T., and Drummond, A. J. (2019). BEAST 2.5: an advanced software platform for Bayesian evolutionary analysis. PLoS Computational Biology 15, e1006650.
| BEAST 2.5: an advanced software platform for Bayesian evolutionary analysis.Crossref | GoogleScholarGoogle Scholar | 31386651PubMed |
Burton, T. E., and Davie, P. J. F. (2007). A revision of the shovel-nosed lobsters of the genus Thenus (Crustacea: Decapoda: Scyllaridae), with descriptions of three new species. Zootaxa 1429, 1–38.
| A revision of the shovel-nosed lobsters of the genus Thenus (Crustacea: Decapoda: Scyllaridae), with descriptions of three new species.Crossref | GoogleScholarGoogle Scholar |
Carmona-Osalde, C., and Rodríguez-Serna, M. (2012). Reproductive aspects of the spider crab Libinia dubia under laboratory conditions. Hidrobiológica 22, 58–61.
Castresana, J. (2000). Selection of conserved clocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540–552.
| Selection of conserved clocks from multiple alignments for their use in phylogenetic analysis.Crossref | GoogleScholarGoogle Scholar | 10742046PubMed |
Chace, F. (1942). Six new species of decapod and stomatopod Crustacea from the Gulf of Mexico. Proceedings of the New England Zoological Club 9, 79–92.
Cházaro-Olvera, S., Carmona-Osalde, C., and Rodríguez-Serna, M. (2020). Larval development of Libinia rhomboidea Streets, 1870 (Crustacea: Decapoda: Brachyura: Majoidea) under laboratory conditions. Nauplius 28, e2020032.
| Larval development of Libinia rhomboidea Streets, 1870 (Crustacea: Decapoda: Brachyura: Majoidea) under laboratory conditions.Crossref | GoogleScholarGoogle Scholar |
Coelho, P. A., and Ramos, M. (1972). A constituição e a distribuição da fauna de decapodos do litoral leste da América do Sul entre as latitudes 5°N e 39°S. Trabalhos Oceanográficos da Universidade Federal de Pernambuco 13, 133–236.
| A constituição e a distribuição da fauna de decapodos do litoral leste da América do Sul entre as latitudes 5°N e 39°S.Crossref | GoogleScholarGoogle Scholar |
Colavite, J., Windsor, A. M., and Santana, W. (2019). Three new species and a new genus of majoid crabs from the eastern Pacific (Decapoda, Brachyura). ZooKeys 825, 1–24.
| Three new species and a new genus of majoid crabs from the eastern Pacific (Decapoda, Brachyura).Crossref | GoogleScholarGoogle Scholar |
Colavite, J., Windsor, A. M., and Santana, W. (2020). A new genus for Pericera septemspinosa Stimpson, 1871 and Pericera heptacantha Bell, 1836 (Crustacea, Brachyura, Majoidea), based on morphology and molecular data. Zoosystematics and Evolution 96, 205–216.
| A new genus for Pericera septemspinosa Stimpson, 1871 and Pericera heptacantha Bell, 1836 (Crustacea, Brachyura, Majoidea), based on morphology and molecular data.Crossref | GoogleScholarGoogle Scholar |
Collins, J. S. H., Garvie, C. L., and Mellish, C. J. T. (2014). Some decapods (Crustacea, Brachyura and Stomatopoda) from the Pleistocene Beaumont Formation of Galveston, Texas. Scripta Geologica 147, 309–329.
Davie, P., Guinot, D., and Ng, P. K. L. (2015a). Phylogeny of Brachyura. In ‘Decapoda: Brachyura (Part 2)’. (Eds P. Castro, P. Davie, D. Guinot, F. Scharan, and C. von Vaupel Klein.) Treatise on Zoology – Anatomy, Taxonomy, Biology, pp. 921–979. (Brill: Leiden, Netherlands.)
Davie, P., Guinot, D., and Ng, P. K. L. (2015b). Systematics and classification of Brachyura. In ‘Decapoda Brachyura (Part 2)’. (Eds P. Castro, P. Davie, D. Guinot, F. Scharan, and C. von Vaupel Klein.) Treatise on Zoology – Anatomy, Taxonomy, Biology, pp. 1049–1130. (Brill: Leiden, Netherlands.)
De Grave, S., Pentcheff, N. D., Ahyong, S. T., Chan, T.-Y., Crandall, K. A., Dworschak, P. C., Felder, D. L., Feldmann, R. M., Fransen, C. H. J. M., Goulding, L. Y. D., Lemaitre, R., Low, M. E. Y., Martin, J. W., Ng, P. K. L., Schweitzer, C. E., Tan, S. H., Tshudy, D., and Wetzer, R. (2009). A classification of living and fossil genera of decapod crustaceans. The Raffles Bulletin of Zoology 21, 1–109.
Dieffenbach, C. W., Lowe, T. M., and Dveksler, G. S. (1993). General concepts for PCR primer design. PCR Methods and Applications 3, S30–S37.
| General concepts for PCR primer design.Crossref | GoogleScholarGoogle Scholar | 8118394PubMed |
Estoup, A., Lagiardèr, C. R., Perrot, E., and Chourrout, D. (1996). Rapid one-tube DNA extraction for reliable PCR detection of fish polymorphic markers and transgenes. Molecular Marine Biology and Biotechnology 5, 295–298.
Felder, D. L., Álvarez, F., Goy, J. W., and Lemaitre, R. (2009). Decapoda (Crustacea) of the Gulf of Mexico, with comments on the Amphionidacea. In ‘Gulf of Mexico Origin, Waters, and Biota. Volume 1, Biodiversity’. (Eds D. L. Felder and D. K. Camp.) pp. 1019–1104. (Texas A&M University Press: College Station, TX, USA.)
Feldmann, R. M., and Schweitzer, C. E. (2016). Giant spider crab from the St Marys Formation (Miocene) in Calvert County, Maryland, USA. Bulletin of the Mizunami Fossil Museum 42, 23–28.
Feldmann, R. M., Schweitzer, C. E., and Bohaska, D. J. (2018). Libinia marylandicus Palmer, 1935: discovery of a forgotten specimen from the Maryland Miocene, USA, and reevaluation of Libinia spp. from the Maryland Miocene. Bulletin of the Mizunami Fossil Museum 44, 1–8.
Felsenstein, F. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. (1994). DNA primers for amplifications of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.
| 7881515PubMed |
Françoso, E., and Arias, M. C. (2013). Cytochrome c oxidase I primers for corbiculate bees: DNA barcode and mini‐barcode. Molecular Ecology Resources 13, 844–850.
| Cytochrome c oxidase I primers for corbiculate bees: DNA barcode and mini‐barcode.Crossref | GoogleScholarGoogle Scholar | 23848578PubMed |
Garth, J. S. (1958). Brachyura of the Pacific coast of America. Oxyrhyncha. Allan Hancock Pacific Expeditions 21, 1–854.
Garth, J. S., and Méndez, M. (1983). A new species of spider crab of the genus Libinia from Peru, and the first known male of Delsolaria enriquei Garth, 1973 (Crustacea, Brachyura, Majidae). Bulletin of the Southern California Academy of Sciences 82, 125–130.
Gonçalves, G. R. L., Wolf, M. R., Costa, R. C., and Castilho, A. L. (2016). Decapod crustacean associations with scyphozoan jellyfish (Rhizostomeae: Pelagiidae) in the southeastern Brazilian coast. Symbiosis 69, 193–198.
| Decapod crustacean associations with scyphozoan jellyfish (Rhizostomeae: Pelagiidae) in the southeastern Brazilian coast.Crossref | GoogleScholarGoogle Scholar |
Hajibabaei, M., Smith, M. A., Janzen, D. H., Rodriguez, J. J., Whitfield, J. B., and Hebert, P. D. (2006). A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes 6, 959–964.
| A minimalist barcode can identify a specimen whose DNA is degraded.Crossref | GoogleScholarGoogle Scholar |
Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.
Hendrickx, M. (1999). Los cangrejos braquiúros (Crustacea: Brachyura: Majoidea y Parthenopoidea) del Pacífico Mexicano. Comisión Nacional para el conocimiento y uso de la biodiversidad, Instituto de Ciencias del Mar y Limnología Universidad Nacional Autónoma de México, México.
Hernandez-Avila, I., Ocaña, F. A., and Pech, D. (2020). Testing marine regional-scale hypotheses along the Yucatan continental shelf using soft-bottom macrofauna. PeerJ 8, e8227.
| Testing marine regional-scale hypotheses along the Yucatan continental shelf using soft-bottom macrofauna.Crossref | GoogleScholarGoogle Scholar | 31915571PubMed |
Holthuis, L. B. (1959). The Crustacea Decapoda of Suriname (Dutch Guiana). Zoölogische Verhandelingen 44, 187–190.
Huelsenbeck, J. P., and Crandall, K. A. (1997). Phylogeny estimation and hypothesis testing using Maximum Likelihood. Annual Review of Ecology and Systematics 28, 437–466.
| Phylogeny estimation and hypothesis testing using Maximum Likelihood.Crossref | GoogleScholarGoogle Scholar |
Huelsenbeck, J. P., and Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.
| MRBAYES: Bayesian inference of phylogeny.Crossref | GoogleScholarGoogle Scholar | 11524383PubMed |
Hultgren, K. M., and Stachowicz, J. J. (2008). Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology. Molecular Phylogenetics and Evolution 48, 986–996.
| Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology.Crossref | GoogleScholarGoogle Scholar | 18621552PubMed |
Hultgren, K. M., Guerao, G., Marques, F. P. L., and Palero, F. (2009). Assessing the contribution of molecular and larval morphological characters in a combined phylogenetic analysis of the Superfamily Majoidea. In ‘Decapod Crustacean Phylogenetics’. (Eds J. W. Martin, K. A. Crandall, and D. L. Felder.) pp. 437–455. (CRC Press: Boca Raton, FL, USA.)
| Crossref |
Iamsuwansuk, A., Denduangboripant, J., and Davie, P. J. F. (2012). Molecular and morphological investigations of shovel-nosed lobsters Thenus spp. (Crustacea: Decapoda: Scyllaridae) in Thailand. Zoological Studies 51, 108–117.
Kameya, A., Moscoso, V., and Lleellish, M. (1998). Los crustáceos decápodos y estomatópodos del Peru. Informe de Instituto del Mar del Peru 136, 80–109.
Katoh, K., Rozewicki, J., and Yamada, K. D. (2017). MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 108, 1–7.
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markwitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P., and Drummond, A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics Applications Note 28, 1647–1649.
| Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.Crossref | GoogleScholarGoogle Scholar |
Kumar, S., Stecher, G., and Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.
| MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets.Crossref | GoogleScholarGoogle Scholar | 27004904PubMed |
Luque, J., Schweitzer, C. E., Santana, W., Portell, R. W., Vega, F. J., and Klompmaker, A. A. (2017). Checklist of fossil decapod crustaceans from tropical America. Part I: Anomura and Brachyura. Nauplius 25, e2017025.
| Checklist of fossil decapod crustaceans from tropical America. Part I: Anomura and Brachyura.Crossref | GoogleScholarGoogle Scholar |
Mantelatto, F. L., Carvalho, F. L., Simões, S. M., Negri, M., Souza-Carvalho, E. A., and Terossi, M. (2016). New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea). Nauplius 24, e2016030.
| New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea).Crossref | GoogleScholarGoogle Scholar |
Mantelatto, F. L., Terossi, M., Negri, M., Buranelli, R. C., Robles, R., Magalhães, T., Tamburus, A. F., Rossi, N., and Miyazaki, M. J. (2018). DNA sequence database as a tool to identify decapod crustaceans on the São Paulo coastline. Mitochondrial DNA – A. DNA Mapping, Sequencing, and Analysis 29, 805–815.
| DNA sequence database as a tool to identify decapod crustaceans on the São Paulo coastline.Crossref | GoogleScholarGoogle Scholar |
Mantelatto, F. L., Tamburus, A. F., Magalhães, T., Buranelli, R. C., Terossi, M., Negri, M., Castilho, A. L., Costa, R. C., and Zara, F. J. (2020). Checklist of decapod crustaceans from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: III. Infraorder Brachyura Latreille, 1802. Zootaxa 4872, 1–108.
| Checklist of decapod crustaceans from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: III. Infraorder Brachyura Latreille, 1802.Crossref | GoogleScholarGoogle Scholar |
Marques, F., and Pohle, G. (1998). The use of structural reduction in phylogenetic reconstruction of decapods and a phylogenetic hypothesis for 15 genera of Majidae: testing previous larval hypotheses and assumptions. Invertebrate Reproduction & Development 33, 241–262.
| The use of structural reduction in phylogenetic reconstruction of decapods and a phylogenetic hypothesis for 15 genera of Majidae: testing previous larval hypotheses and assumptions.Crossref | GoogleScholarGoogle Scholar |
Marques, F., and Pohle, G. (2003). Searching for larval support for majoid families (Crustacea: Brachyura) with particular reference to Inachoididae Dana, 1851. Invertebrate Reproduction & Development 43, 71–82.
| Searching for larval support for majoid families (Crustacea: Brachyura) with particular reference to Inachoididae Dana, 1851.Crossref | GoogleScholarGoogle Scholar |
Martin, J. W., and Davis, G. E. (2001). An update classification of the recent Crustacea. Natural History Museum of Los Angeles County Science Series 39, 1–124.
Melo, G. A. S. (1996). ‘Manual de Identificação dos Brachyura (Caranguejos e Siris) do Litoral Brasileiro.’ (Editora Plêiade/FAPESP: São Paulo, Brazil.)
Miller, S. A., Dykes, D. D., and Polesky, H. F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215.
| A simple salting out procedure for extracting DNA from human nucleated cells.Crossref | GoogleScholarGoogle Scholar | 3344216PubMed |
Miller, M. A., Pfeiffer, W., and Schwartz, T. (2010). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In ‘Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, LA, USA’. INSPEC Accession Number 11705685, pp. 1–8. (IEEE.)
Miranda, I., and Mantelatto, F. L. (2016). Porcelain crabs of the genera Pachycheles Stimpson and Neopisosoma Haig (Decapoda: Anomura: Porcellanidae): new premises based on molecular data and comments on phylogenetic relationships in the family. Invertebrate Systematics 30, 509–520.
| Porcelain crabs of the genera Pachycheles Stimpson and Neopisosoma Haig (Decapoda: Anomura: Porcellanidae): new premises based on molecular data and comments on phylogenetic relationships in the family.Crossref | GoogleScholarGoogle Scholar |
Ng, P. K. L., Guinot, D., and Davie, P. (2008). Systema Brachyurorum: part I. An annotated checklist of extant brachyuran crabs of the world. The Raffles Bulletin of Zoology 17, 1–286.
O’Dea, A., Lessios, H. A., Coates, E. R. I., Restrepo-Moreno, S. A., Cione, A. L., Collins, L. S., De Queiroz, A., Farris, D. W., Norris, R. D., Stallard, R. F., Woodburne, M. O., Aguilera, O., Aubry, M., Berggren, W. A., Budd, A. F., Cozzuol, M. A., Coppard, S. E., Duque-Caro, H., Finnegan, S., Gasparini, G. M., Grossman, E. L., Johnson, K. G., Keigwin, L. D., Knowlton, N., Leigh, E. G., Leonard-Pingel, J. S., Marko, P. B., Pyenson, N. D., Rachello-Dolmen, P. G., Soibelzon, E., Soibelzon, L., Todd, J. A., Vermeij, G. J., and Jackson, J. B. C. (2016). Formation of the Isthmus of Panama. Science Advances 2, e1600883.
| Formation of the Isthmus of Panama.Crossref | GoogleScholarGoogle Scholar | 27540590PubMed |
Pappalardo, P., Pringle, J. M., Wares, J. P., and Byers, J. E. (2015). The location, strength, and mechanisms behind marine biogeographic boundaries of the east coast of North America. Ecography 38, 722–731.
| The location, strength, and mechanisms behind marine biogeographic boundaries of the east coast of North America.Crossref | GoogleScholarGoogle Scholar |
Pohle, G., and Marques, F. (2000). Larval stages of Paradasygyius depressus (Bell, 1835) (Crustacea: Decapoda: Brachyura: Majidae) and a phylogenetic analysis for 21 genera of Majidae. Proceedings of the Biological Society of Washington 113, 739–760.
Powers, L. W. (1977). A catalogue and bibliography to the crabs (Brachyura) of the Gulf of Mexico. Contributions in Marine Science 20, 1–190.
Rathbun, M. J. (1925). The spider crabs of America. Bulletin – United States National Museum 129, 1–613.
Robles, R., Dworschak, P. C., Felder, D. L., Poore, G. C., and Mantelatto, F. L. (2020). A molecular phylogeny of Callianassidae and related families (Crustacea: Decapoda: Axiidea) with morphological support. Invertebrate Systematics 34, 113–132.
| A molecular phylogeny of Callianassidae and related families (Crustacea: Decapoda: Axiidea) with morphological support.Crossref | GoogleScholarGoogle Scholar |
Schneider, H. (2017). ‘Métodos de Análise Filogenética’, 4° edição. (Chiado Editora.)
Schubart, C. D. (2011). Reconstruction of phylogenetic relationships within Grapsidae (Crustacea: Brachyura) and comparison of trans-isthmian versus amphi-atlantic gene flow based on mtDNA. Zoologischer Anzeiger 250, 472–478.
| Reconstruction of phylogenetic relationships within Grapsidae (Crustacea: Brachyura) and comparison of trans-isthmian versus amphi-atlantic gene flow based on mtDNA.Crossref | GoogleScholarGoogle Scholar |
Schubart, C. D., and Huber, M. G. J. (2006). Genetic comparisons of German populations of the stone crayfish, Austropotamobius torrentium (Crustacea: Astacidae). Bulletin Francais de la Peche et de la Pisciculture 380–381, 1019–1028.
| Genetic comparisons of German populations of the stone crayfish, Austropotamobius torrentium (Crustacea: Astacidae).Crossref | GoogleScholarGoogle Scholar |
Spivak, E. D., Farías, N. E., Ocampo, E. H., Lovrich, G. A., and Luppi, T. A. (2019). Annotated catalogue and bibliography of marine and estuarine shrimps, lobsters, crabs and their allies (Crustacea: Decapoda) of Argentina and Uruguay (Southwestern Atlantic Ocean). Frente Marítimo 26, 1–164.
Stamatakis, A. (2014). RAxML Version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313.
| RAxML Version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.Crossref | GoogleScholarGoogle Scholar | 24451623PubMed |
Talavera, G., and Castresana, J. (2007). Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56, 564–577.
| Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments.Crossref | GoogleScholarGoogle Scholar | 17654362PubMed |
Tavares, M., and Santana, W. (2011). A new genus for Libinia rostrata Bell, 1835, with comments on the validity of Libinia bellicosa Oliveira, 1944 (Crustacea, Brachyura, Majoidea, Epialtidae). Zootaxa 3057, 61–68.
| A new genus for Libinia rostrata Bell, 1835, with comments on the validity of Libinia bellicosa Oliveira, 1944 (Crustacea, Brachyura, Majoidea, Epialtidae).Crossref | GoogleScholarGoogle Scholar |
Tavares, M., and Santana, W. (2012). On the morphological differentiation between Libinia spinosa and L. ferreirae (Crustacea: Brachyura: Majoidea: Epialtidae). Zoologia 29, 577–588.
| On the morphological differentiation between Libinia spinosa and L. ferreirae (Crustacea: Brachyura: Majoidea: Epialtidae).Crossref | GoogleScholarGoogle Scholar |
Tsang, L. M., Schubart, C. D., Ahyong, S. T., Lai, J. C. Y., Au, E. Y. C., Chan, T. Y., Ng, P. K. L., and Chu, K. H. (2014). Evolutionary history of true crabs (Crustacea: Decapoda: Brachyura) and the origin of freshwater crabs. Molecular Biology and Evolution 31, 1173–1187.
| Evolutionary history of true crabs (Crustacea: Decapoda: Brachyura) and the origin of freshwater crabs.Crossref | GoogleScholarGoogle Scholar | 24520090PubMed |
Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B. C., Remm, M., and Rozen, S. G. (2012). Primer3 – new capabilities and interfaces. Nucleic Acids Research 40, e115.
| Primer3 – new capabilities and interfaces.Crossref | GoogleScholarGoogle Scholar | 22730293PubMed |
Williams, A. B. (1984). ‘Shrimps, Lobsters, and Crabs of the Atlantic Coast of the Eastern United States, Maine to Florida.’ (Smithsonian Institution Press: Washington, DC, USA.)
Windsor, A. M., and Felder, D. L. (2014). Molecular phylogenetics and taxonomic reanalysis of the family Mithracidae MacLeay (Decapoda: Brachyura: Majoidea). Invertebrate Systematics 28, 145–173.
| Molecular phylogenetics and taxonomic reanalysis of the family Mithracidae MacLeay (Decapoda: Brachyura: Majoidea).Crossref | GoogleScholarGoogle Scholar |
WoRMS (2021). Libinia Leach, 1815. In ‘World Register of Marine Species’. Available at http://www.marinespecies.org/aphia.php?p=taxdetailsandid=106906 [Verified 26 January 2021].
