Morphology and DNA barcodes reveal the presence of the non-native land planarian Obama marmorata (Platyhelminthes : Geoplanidae) in Europe
Domingo Lago-Barcia A , Fernando A. Fernández-Álvarez A B F , Lisandro Negrete C D , Francisco Brusa C D , Cristina Damborenea C D , Cristina Grande E and Carolina Noreña A
+ Author Affiliations
- Author Affiliations
A Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (CSIC), Madrid, Spain.
B Institut de Ciències del Mar (CSIC), Passeig Maritim 37–49, 08003 Barcelona, Spain.
C División Zoología Invertebrados, Museo de La Plata (FCNyM-UNLP), Paseo del Bosque s/n, La Plata, Buenos Aires, Argentina.
D CONICET – Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina).
E Departamento de Biología Molecular and Centro de Biología Molecular ‘Severo Ochoa’ (Consejo Superior de Investigaciones Científicas), Universidad Autónoma de Madrid, 28049 Madrid, Spain.
F Corresponding author. Email: f.a.fernandez.alvarez@gmail.com
Invertebrate Systematics 29(1) 12-22 https://doi.org/10.1071/IS14033
Submitted: 9 July 2014 Accepted: 20 October 2014 Published: 20 March 2015
Abstract
We report for the first time the occurrence of Obama marmorata in the Iberian Peninsula as an introduced species from the Neotropical Region. The species is also reported for the first time in Argentina. The identification was made on the basis of morphological evidence. The divergence levels of a fragment of the mitochondrial cytochrome c oxidase I gene from Argentinean and Iberian samples were studied. The morphology of samples from the two regions was the same and matched the original description of the species. A DNA barcoding matrix was constructed using new sequences from O. marmorata and sequences of related species taken from GenBank. Among the Geoplanidae, interspecific divergence ranged between 3.3 and 14.4%, while intraspecific divergence percentages were 0–1.2%, signalling the presence of a DNA barcoding gap. All O. marmorata sequences, irrespective of their geographic origin, form a well supported clade with an intraspecific divergence of 0–0.9 (average = 0.4%). These results indicate the utility of DNA barcoding to discover allochthonous species in this group of organisms.
Additional keywords: Argentina, exotic species, Geoplaninae, Spain, terrestrial flatworm.
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