Pilumnus Leach (Decapoda : Pilumnidae Samouelle) from Mediterranean and adjacent Atlantic waters: a COI analysis
Ulla Oliveira-Biener A , Roland R. Melzer A C and Michael A. Miller A BA Zoologische Staatssammlung, Münchhausenstr. 21, D-81247 München, Germany.
B kmbioservices, Ferdinand-Zwack-Str. 39, D-85354 Freising, Germany.
C Corresponding author. Email: melzer@zsm.mwn.de
Invertebrate Systematics 24(2) 182-193 https://doi.org/10.1071/IS09051
Submitted: 19 December 2009 Accepted: 20 April 2010 Published: 29 June 2010
Abstract
A molecular taxonomy is presented based on 544 base pair sequences of the mitochondrial COI gene (Palumbi segment) from 141 bristle crabs of the genus Pilumnus from the Mediterranean Sea and adjacent Atlantic Ocean, plus outgroups. The sample includes all currently valid autochthonous Mediterranean species. Six well-supported COI lineages were detected, three of which exclusively comprise representatives of Pilumnus inermis, P. spinifer and P. villosissimus, respectively. On the other hand, crabs that would be attributed to P. hirtellus according to current morphology-based taxonomy were distributed over three COI clades, two of which are interpreted as representing cryptic lineages. We compare our findings to previous analyses using sets of external morphological characters, and discuss the partial mismatch between the morphology- and gene-based classifications.
Additional keywords: DNA taxonomy, COI, species delimitation, sets of characters, cryptic lineages, Pilumnus.
Acknowledgements
We thank Stefan Friedrich (Munich) for expert technical assistance and collection management. Special thanks go to Peter Wirtz (Madeira), Roland Anton, Hannes Geiselbrecht, Martin Hess, Thomas Knebelsberger, Tobias Lehmann, Roland Meyer and Isabella Stöger (Munich), Christoph Schubart (Regensburg), Davide Tagliapetra (Venice), Peter Ng and Joelle Chiu Yun Lai (Singapore) and to the staff of the Station Biologique (Roscoff) for collecting and/or contributing specimens for this study. We are indebted to Christoph Schubart (Regensburg) for helpful discussions and for providing us with aliquots of some of his Pilumnus DNA samples. Special thanks go to M. Spiess (München) for polishing the English. This study was partly supported by the DNA storage unit at ZSM.
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