The role of the chloroplast localised phosphate transporter GmPHT4;10 gene in plant growth, photosynthesis and drought resistance
Liwei Liu A , Xu He A , Shuwen Wang A , Xueting Qin A , Songhao Che A , Lei Wu A , Dongchao Wang B , Ping Tian B , Xiaoshuang Wei B , Zhihai Wu B , Xue Yang
A * and Meiying Yang A *
+ Author Affiliations
- Author Affiliations
A College of Life Sciences, Jilin Agricultural University, Changchun, Jilin 130118, China.
B College of Agronomy, Jilin Agricultural University, Changchun, Jilin 130118, China.
Functional Plant Biology 50(8) 649-662 https://doi.org/10.1071/FP23008
Submitted: 9 January 2023 Accepted: 19 May 2023 Published: 13 June 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
In view of the importance of inorganic phosphate to plant growth and development, the role of phosphate transporters responsible for absorption and transportation in crops has attracted increasing attention. In this study, bioinformatics analysis and subcellular localisation experiment showed that GmPHT4;10 is a member of PHT4 subfamily of phosphate transporters and located in chloroplasts. The gene was induced by phosphate deficiency and drought, and was the highest in leaves. After GmPHT4;10 gene was replenished to AtPHT4;5 gene deletion mutant lines (atpht4;5), the phenotype of the transgenic lines was basically recovered to the level of wild-type, but there were significant differences in phosphate content and photosynthetic indicators between wild-type and revertant lines. Meanwhile, the difference of proline content and catalase activity between the two lines also indicated that GmPHT4;10 gene and its orthologous gene AtPHT4;5 were different in drought resistance and drought resistance mechanism. After overexpression of GmPHT4;10 gene in Arabidopsis thaliana, more phosphate and proline were accumulated in chloroplasts and catalase activity was increased, thus improving photosynthesis and drought resistance of plants. The results further supplement the cognition of PHT4 subfamily function, and provides new ideas and ways to improve photosynthesis by revealing the function of chloroplast phosphate transporter.
Keywords: drought stress, functional differentiation, Glycine max, orthologous gene, phosphate deficiency, phosphate transporter, photosynthesis, transgenic plants.
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