Proteomic profiling uncovered the cytosolic superoxide dismutase BsSOD1 associated with plant defence in the herbal orchid Bletilla striata
Bao-Wei Lu A , Feng-Xia An
A E , Liang-Jing Cao B , Yong-Jian Yang A , Peng-Ming Liu A , Xuan Wang B , Bao-Liang Yang A , Yu-Lei Zhang B C , Yan-Feng Ding D and Jun Liu B E
+ Author Affiliations
- Author Affiliations
A School of Chinese Medicine, Bozhou University, Bozhou, 236800, China.
B National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
C Wanbei Pharmaceutical Co. of Bozhou City Co. Ltd, Bozhou, 236800, China.
D Laha Town Health Center, Qiqihar, 161342, China.
E Corresponding authors. Email: an_fengxia@163.com; liujun@caas.cn
Functional Plant Biology 47(10) 937-944 https://doi.org/10.1071/FP19345
Submitted: 2 December 2019 Accepted: 29 April 2020 Published: 26 June 2020
Abstract
The herbal orchid Bletilla striata (Thunb.) Rchb.f. has a long cultivation history and has been widely used in medicines and cosmetics. The fungal infection leaf blight (LB) seriously threatens B. striata cultivation. Here, we systemically collected wild B. striata accessions and isolated the accessions with strong resistance against LB. We carried out proteomic profiling analysis of LB-resistant and LB-susceptible accessions, and identified a large number of differentially expressed proteins with significant gene ontology enrichment for ‘oxidoreductase activity.’ Of the proteins identified in the reactive oxygen species signalling pathway, the protein abundance of the Cu–Zn superoxide dismutase BsSOD1 and its gene expression level were higher in LB-resistant accessions than in LB-susceptible lines. Transient expression of the dismutase fused with yellow fluorescent protein determined that its subcellular localisation is in the cytoplasm. Our study provides new insights into the molecular markers associated with fungal infection in B. striata.
Additional keywords: disease resistance, leaf blight, protoplast transformation.
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