NAC transcription factor involves in regulating bacterial wilt resistance in potato
Yannan Chang A , Ruimin Yu A , Jinlin Feng B , Huize Chen B , Hemu Eri C and Gang Gao
A D
+ Author Affiliations
- Author Affiliations
A Genetic Engineering Laboratory, College of Life Science, Shanxi Normal University, Linfen 041000, China.
B Cell Biology Laboratory, College of Life Science, Shanxi Normal University, Linfen 041000, China.
C Function Food Laboratory, College of Food Science, Shanxi Normal University, Linfen 041000, China.
D Corresponding author. Email: ggsxnu@126.com
Functional Plant Biology 47(10) 925-936 https://doi.org/10.1071/FP19331
Submitted: 17 November 2019 Accepted: 27 April 2020 Published: 27 May 2020
Abstract
Bacterial wilt (BW) is a serious disease that affects potato (Solanum tuberosum L.) production. Although resistance to this disease has been reported, the underlying mechanism is unknown. In this study, we identified a NAC family transcription factor (StNACb4) from potato and characterised its structure, function, expression, its localisation at the tissue and its role in BW resistance. To this end, the transgenic Nicotiana benthamiana Domin lines were generated in which the expression of NACb4 was constitutively upregulated or suppressed using RNAi. Different tobacco mutants were stained after inoculating with Ralstonia solanacearum to observe the cell death and callose deposition. The results indicated that StNACb4 could be upregulated under the induction of R. solanacearum, and salicylic acid, abscisic acid and methyl jasmonate could also induce the expression of StNACb4. Tissue localisation analysis indicated that its expression was tissue specific, and it was mainly in the phloem of the vascular system of stems and leaves. NbNACb4 gene silencing can enhance the sensitivity of tobacco to R. solanacearum; on the contrary, StNACb4 gene overexpression can enhance the tolerance of tobacco to R. solanacearum. Meanwhile, StNACb4 gene overexpression can induce cell death and callose deposition in tobacco. The upregulated expression of StNACb4 can also activate the StPR10 gene expression. Our results provide important new insights into the regulatory mechanisms of bacterial wilt resistance in potato.
Additional keywords: callose deposition, cell death, Ralstonia solanacearum, StNACb4, transgenic tobacco.
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