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RESEARCH ARTICLE
Contents Vol 34(10)

The effects of UV-B radiation on photosynthesis in relation to Photosystem II photochemistry, thermal dissipation and antioxidant defenses in winter wheat (Triticum aestivum L.) seedlings at different growth temperatures

Shu-Hua Yang A B D , Li-Jun Wang A D , Shao-Hua Li A E , Wei Duan A , Wayne Loescher C and Zhen-Chang Liang A B
+ Author Affiliations
- Author Affiliations

A Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, P.R. China.

B Graduate School of the Chinese Academy of Sciences, Beijing 100049, P.R. China.

C College of Agriculture and Natural Resources, Michigan State University, East Lansing 48824, USA.

D These authors contributed equally to the paper.

E Corresponding author. Email: shhli@ibcas.ac.cn

Functional Plant Biology 34(10) 907-917 https://doi.org/10.1071/FP07083
Submitted: 9 April 2007  Accepted: 31 July 2007   Published: 13 September 2007

Abstract

To study the UV-B effect on photosynthesis in winter wheat at different day/night temperatures, biologically effective UV-B radiation at 4.2 (LUVB) and 10.3 (HUVB) kJ m–2 d–1 was provided on the seedlings at 25/20°C or 10/5°C. UV-B radiation inhibited net photosynthesis rate (Pn) by enhanced intensity and decreased temperature without change of intercellular CO2 concentrations (Ci). Decreased maximal quantum yield of Photosystem II (Fv/Fm) and increased minimum fluorescence (Fo) were observed in HUVB at both temperatures and LUVB at 10/5°C. HUVB increased total pool size (VAZ) of xanthophyll cycle pigments, but decreased the de-epoxidation state (DEPS) of these pigments at both temperatures, while LUVB only decreased DEPS at 10/5°C. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) and the redox states of ascorbate and glutathione (AsA/DAsA and GSH/GSSG) were enhanced at 25/20°C, while there were increased SOD and CAT, unaltered APX activities and AsA/DHA, as well as decreased GR activity and GSH/GSSG in LUVB and HUVB at 10/5°C. UV-B radiation resulted in higher H2O2 and thiobarbituric acid reactive substance (TBARS) concentrations at 10/5°C than 25/20°C. It appears that low temperature alone did not influence photosynthesis but aggravated UV-B induced photoinhibition, which was associated with PSII photochemistry rather than stomatal limitation. Xanthophyll cycle pigments failed to provide photoprotection through thermal dissipation. The antioxidant system was up-regulated in LUVB and HUVB at 25/20°C, but was impaired at 10/5°C. Low temperature intensified UV-B induced photoinhibition and damage by weakening the antioxidant system.

Additional keywords: antioxidant system, chlorophyll fluorescence, lipid peroxidation, xanthophyll cycle.


Acknowledgements

The research was supported from the National Natural Science Foundation of China (No.30300057/C011109). We thank Prof. Douglas D. Archbold, University of Kentucky, USA, and Prof. Shou-Ren Zhang, Institute of Botany, the Chinese Academy of Sciences, for critical reviews of the manuscript. We also thank Jian Cui and Wei Huang for their extra work during the experiments.


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